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Explain the methodology, rationale, and challenges in the control of Triatomine vectors.
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Solved in 2 steps
- . Throughout downstream processing, various analytical methods must be used to evaluate the effectiveness of unit operations. Provide a method or methods (if more than one is required) for each scenario. Quick determination of the concentration of a purified sample of monoclonal antibody. Determine the concentration of monoclonal antibody in clarified extract. Study aggregation of a protein product without disruption of tertiary protein structure. Characterize phenolics (secondary metabolites and impurities produced by green plant tissue) by identifying phenolic species and relative concentrations.Describe the rationale behind the electrophoretic mobility shift assay.For what types of problems is the SR method preferred over the BP method?
- Drug substances are useful based on their biological activity. Thus, an assay must be developed to ensure that this important function is preserved during purification. There are three major types of biological activity assays including animal model assays, cell-line-derived assays, and in vitro biochemical assays. Please find an example of each (in literature, online, etc.) and briefly Include a reference for each (no particular reference format required, can simply include a web link if easiest).Define vector. Give the properties of a “Good Vector”.a. Briefly discuss (using three sentences) how the concepts and/or techniques in molecular biology are applied in COVID-19 pandemic, and: b. For each topic (Covid-19 pandemic), give any issues, benefits, or drawbacks of the application (still using three sentences).
- Why is NHEJ an error-prone mechanism?Detail of basic steps involved in synthesis of a recombinant drug product. Justify by manufacturing protocol of recombinant insulin.What are the different categories of cell viability assays? Describe one of them briefly. Define the role (aim of use) of Trypan blue dye in cell culture studies.
- state what the ethical problems are in the case of covid 19 of the challenge testing? List Three reason? what are the problem? How are those terms problems ? What are the problems with them?Why clinical trials are necessary? Explain various clinical trial designs and phases of clinical trials.We discussed various assay attributes in class which provide insight into the effectiveness or suitability of a given analytical method. As part of assay development, various attributes were investigated. For each description below, indicate which assay attribute was being evaluated (partial list of attributes include precision, accuracy, specificity, linearity, LOQ/LOD, etc.). The impact of assay temperature and pH on assay results were evaluated to find a suitable range of conditions for the activity assay. Various known concentrations of standard α-AM solutions were evaluated, and the proportionality of the responses were determined. A reference standard of known α-AM (alpha-amylase) concentration was evaluated in triplicate to determine the variability (or scatter) of repeat measurements. Spiked standard of known specific activity and purity was spiked into a sample and the ability for the assay to measure the true biological activity was determined. The impact of various extract…
