log (10)-1-2-3-23Slope = 1Slope = 1------20log po₂Slope = 2.5123 9.Shown below are the Hill plots of myoglobin (cyan) and an unknown 02 binding protein (magenta).(a) Does this unknown protein bind to 02 cooperatively? If so, is it a positive or negativecooperativity?(b) Does binding of the 1st 02 increase or decrease the affinity for the 2nd O2?(c)What is the minimal number of 02 binding sites that this unknown protein has? Why?

a. Does this unknown protein bind to O2 cooperatively? If so, is it a positive or negative…

Answered: log (10) -1 -2 -3 - 2 3 Slope = 1 Slope…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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2. The Ca2+ ATPase pumps on the sarcoplasmic reticulum membrane play an important role in muscle contraction. In patients with muscular dystrophy, there is some evidence that shows the expression of the Ca2+ ATPase pumps is diminished (there are fewer). Which of the following cellular effects may be a result of the diminished number of Ca2+ ATPase pumps? A)Reduced Ca2+ release through the RyR B)Reduced Ca2+ uptake into the SR C)Slowed activity of the Na/K pump D)Reduced communication between the DHP receptor and the Ca2+ ATPase pumps E)Reduced communication between the DHP Receptor and the Ryanodine receptor
1. In fetal hemoglobin, residue 143 of the beta subunit is a serine rather than the histidine found in adult hemoglobin (see figure on page 9). a) What does this do to the charges in the BPG binding cavity? b) Would you expect this to change the oxygen affinity of fetal hemoglobin, and, if so, in what direction?
3) Acetylcholine binds to a GPCR on heart muscle, making the heart beat more slowly. The activated receptor stimulates a G protein, which opens a K+ channel in the plasma membrane, as shown in Figure 1. Which of the following would enhance this effect of the acetylcholine? (a) (b) (c) (d) Figure 1 addition of a high concentration of a non-hydrolyzable analog of GTP addition of a drug that prevents the a subunit from exchanging GDP for GTP mutations in the acetylcholine receptor that weaken the interaction between the receptor and acetylcholine mutations in the acetylcholine receptor that weaken the interaction between the receptor and the G protein
21. Which protein structure is most consistent for the hydropathy graph of the intrinsic membrane protein shown below? +168 F +84 -84 -168 20 100 200 300 First amino acid in window A) A seven-helix serpentine receptor B) A Type I integral membrane protein C) A Type Il integral membrane protein D) A GPI-anchored protein E) A beta-barrel protein with about 10 membrane-spanning strands. Hydropathy index (kJ mol-1)
6. A quantitative amino acid analysis reveals that bovine serum albumin (BSA) contains 0.58% tryptophan (MW=204) by weight. (a) Calculate the minimum molecular weight of BSA (i.e., assume there is only one Trp residue per protein molecule). (b) Gel filtration of BSA gives a molecular weight estimate of 70,000. How many Trp residues are present in a molecule of serum albumin?
A quantitative amino acid analysis reveals that bovine serum albumin (BSA) contains 0.58% tryptophan (Mr 204) by weight.(a) Calculate the minimum molecular weight of BSA (i.e., assume there is only one Trp residue per protein molecule).(b) Size-exclusion chromatography of BSA gives a molecular weight estimate of 70,000. How many Trp residues are present in a molecule of serum albumin?
What are the four components for the translocation of protein across endoplasmic reticulum? and briefly explain ORF?
5. Describe the distinct SH domains and their binding specificities. How are SH domains in regulating the specificity of siganling?
6. For a 1:1 ligand-receptor binding interaction that is modeled by X + Y XY : (A) Show how the fraction of ligand-receptor binding (fo) is represented by the [X] [X]+ K, equation of f, (i.e., derive this expression for this reaction beginning eg with the relationship for K = f([X].[X],[XY]) under equilibrium conditions). (B) shown to the right for this reaction, what is the estimated value of Keg? Give your answer in units of M. For the data plot 0.9 0.8 0.7 0.6 문 0.5 0.4 0.3 0.2 0.1 0.0000 0.0050 0.0100 0.0150 0.0200 0.0250 0.0300 0.0350 0.0400 [X) (mM)
1. Which myosin type II property is most different from kinesin-1? A) myosin has two heads that can bind to cytoskeletal filamentsB) myosin is recruited into thick bipolar assembliesC) myosin hydrolyzes ATPD) myosin is an allosteric motor 2. If you were examining a newly available genome and your task was to identify the encoded family of kinesin motor proteins, what domain would be the most useful starting place for your analysis? A) Light chain #1 binding sitesB) Neck regionC) Cargo domainD) Motor domainE) Light chain #2 binding sites please explain
A mutation in the alpha globin gene that introduced one additional basic amino acid residue in the BPG binding pocket would: (select one) a) Eliminate Hb’s positive cooperativity. b) Decrease Hb’s O2 binding affinity. c) Increase Hb’s O2 binding affinity. d) Increase O2 binding in muscle and decrease it in lungs. e) Decrease O2 binding in muscle and increase it in lungs. f) It would have no effect on the adult form of Hb.
3. When you think about the primary structure of the mannose-6-P receptor, assuming that it is an integral membrane protein with one membrane-spanning domain and assuming that it has a ERSS, what are all of the different protein domains that must be present in this protein's primary structure to get it to where it needs to go and to carry out all of the functions/activities? (Draw a schematic of the protein structure/sequence and explain each region).

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