Swer sections C, D and E in the same answer book. ction D (Chromatography) The following data were obtained for the analysis of a mixture of compounds (A-D) by HPLC: Peak Retention time (min) non-retained Width of peak base (min) 0.51 B 11.3 1.03 C 13.1 1.25 D 21.6 1.62 a) Using peak C, calculate the number of plates (N) in this column. b) For peak D, calculate the capacity factor (k'). c) For peak B and C, calculate the resolution (R.).
Swer sections C, D and E in the same answer book. ction D (Chromatography) The following data were obtained for the analysis of a mixture of compounds (A-D) by HPLC: Peak Retention time (min) non-retained Width of peak base (min) 0.51 B 11.3 1.03 C 13.1 1.25 D 21.6 1.62 a) Using peak C, calculate the number of plates (N) in this column. b) For peak D, calculate the capacity factor (k'). c) For peak B and C, calculate the resolution (R.).
Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter26: An Introduction To Chromatographic Separations
Section: Chapter Questions
Problem 26.14QAP
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Question
![Answer sections C, D and E in the same answer book.
Section D (Chromatography)
Question 8
The following data were obtained for the analysis of a mixture of compounds (A-D) by
HPLC:
Peak
Retention time (min) | Width of peak base (min)
non-retained
2.1
A
6.4
10.51
B
11.3
1.03
C
13.1
1.25
D
21.6
1.62
a) Using peak C. calculate the number of plates (N) in this column.
b) For peak D, calculate the capacity factor (k').
c) For peak B and C, calculate the resolution (R).](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F122eb431-f16a-43cb-8178-a9349a632f30%2F40e1757d-2561-4520-980c-3ea1faf7cd53%2F3td8os_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Answer sections C, D and E in the same answer book.
Section D (Chromatography)
Question 8
The following data were obtained for the analysis of a mixture of compounds (A-D) by
HPLC:
Peak
Retention time (min) | Width of peak base (min)
non-retained
2.1
A
6.4
10.51
B
11.3
1.03
C
13.1
1.25
D
21.6
1.62
a) Using peak C. calculate the number of plates (N) in this column.
b) For peak D, calculate the capacity factor (k').
c) For peak B and C, calculate the resolution (R).
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