In the first step of your phage therapy design process, you isolate the exact Mycobacterium strain from the patient. What is the most important thing must you establish next about this bacterial isolate?
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In the first step of your phage therapy design process, you isolate the exact Mycobacterium strain from the patient. What is the most important thing must you establish next about this bacterial isolate?
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- You've been assigned with isolating a certain species of bacteria from a mixed culture in order to characterize and identify the strain. Which technique(s) is/are the BEST to utilize? How will the procedure be carried out? Explain.The images attached are the photos of bacteria in yoghurt under a misroscope. According to these images and your own knowledge, can you make a biological drawing of bacteria in yoghurt ( in a circle) and identify the types of bacteria accurately?Which of the following is TRUE when one assay bacteriophage titers? You should: a) first mix the phages with a live bacterial culture and then pour the mixture on the agar plate b) directly add the phage dilution onto the surface of an agar plate c) add tryptic soy broth to the phage dilution and incubate overnight d) incubate a phage solution with live bacterial cells for several minutes. You must add soft agar to the mixture before pouring the content on the agar plate
- The following pictures show the results of a Disk Diffusion Assay for different types of bacteria. For each bacteria, what antibiotic would you recommend be used on the patient? Explain your choice.You have conducted serial 10-fold dilutions and measured the cfu (colony forming units) of a Streptococcus pneumoniae culture and also the pfu (plaque forming units) of a phage (virus) that infects the bacteria. You counted 5 cfu in a 0.4 ml sample of a 106 dilution of the bacterial sample. You then counted 50 plaque-forming units (pfu) in a 0.25 ml sample of a 108 diluted sample of the phage culture. What are the cfu/ml of the S. pneumoniae and pfu/ml of the phage cultures before dilution? 5 x 106 cfu/ml and 2 x 109 pfu/ml 4 x 107 CFU/ml and 2 x 109 PFU/ml 1.25 x 108 cfu/ml and 10 x 1010 pfu/ml 1.25 x 107 cfu/ml and 2 x 1010 pfu/mlYou have used morphological observations and biochemical tests to identify two unknown bacterial isolates in your practical sessions, briefly describe three other techniques that can be used to identify unknown bacteria (you can use any reliable online resources). Note: These must not be based on biochemical tests (enzyme use, acid production etc) or morphological characteristics (size, shape, structures).
- What is the importance of the agar overlay technique in virus isolation?You are attempting to propagate bacteriophage of Bacillus cereus using a liquid batch culture. A growing culture of B. cereus is inoculated with your bacteriophage. The day before you tested the bacteria and the phage batch and they both behaved as expected. You made sure that all containers are labeled appropriately. Immediately after inoculation, you take a sample but are unable to detect any bacteriophage in the media. What is the most likely explanation? O You used the wrong bacteria or the wrong bacteriophage. There is an issue with the phage batch. O There is a problem with your bacterial culture of B. cereus. O It is too soon. The bacteriophages are still replicating and assembling inside the bacterial cells and are therefore not detectable in the growth media.Would you use an inoculating loop to transfer bacteria to an agar deep tube? Would you use an inoculating loop to transfer bacteria to an agar deep tube? Yes, as long as you insert the loop into the agar smoothly. No, a needle would be best since it will do the least amount of damage to the agar and allow it to reseal around the inserted bacteria. It depends on which bacteria you are transferring. Yes, as long as you are careful when streaking on the surface of the agar.
- Why is pure culture and culture collection important in identifying novel species of bacteria?You decide to sample the bacteria growing on several items in a laboratory space. You follow aseptic techniques and use applicator swabs dipped in a sterible broth solution to sample from the floor, the sink, and your shoe. You spread each sample on separate labeled agar plates for incubation. a. You want to be sure that the sterile solution you used for sampling was not contaminated. How would you set up a negative control to test this? b. What result for your negative control would indicate that something is contaminated?The process of collecting characteristics of your unknown bacterium can be helped by the use of a dichotomous key. A dichotomous key is a flowchart that can be used to identify an organism. a) in your own words explain how to create and use a dichotomous key to identify unknown bacteria