Lab 7_Yeast Invertase Worksheet Fa 20

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San Jose State University *

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113A

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Biology

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Apr 28, 2024

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Name:___________________________________________ Date:______________________ Sec,on#:_________ LAB #7: Yeast Invertase Worksheet 1. Choose 1 variable: Enzyme Concentra,on, pH, or Temperature. Fill in your Data Table in the same format as the one in your lab manual. (1 pt) 2. Experimental variable you are tes,ng:_____________Temperature__________________ 3. Plot your results following the direc,ons in the lab manual. Be sure to label all axes. You can use a different piece of paper/graphing paper if you like. (2 pts) Tube # Group 5 Abs Group 6 Abs 1A NO E 0.003 0.002 1B 0.034 0.044 2A NO E 0.000 0.002 2B 0.054 0.073 3A NO E 0.002 0.003 3B 0.164 0.165 4A NO E 0.001 0.004 4B 0.023 0.014 5A NO E 0.002 0.028 5B 0.010 0.012
Name:___________________________________ Date:_________________ Sec,on#:_____________ 4. At which enzyme concentra,on / pH / temperature is invertase ac,vity the greatest based on your experimental results (only answer for the experiment you completed). (1 pt) For the no enzyme its the highest at O degrees and with the enzyme its the highest at 45 degrees. 5. What was the purpose of all the steps where you boiled the samples? (1 pt) The purpose of boiling the samples was to stop then enzyme acAvity from going any further aCer it reached opAmal acAvity in the water bath 6. What tube or tubes are your nega,ve controls? Why are they considered a nega,ve control? (2 pts) The negaAve controls were the tubes without the invertase enzyme. They are considered the negaAve control because it did not contain any invertase 7. Why do you expect the rate of enzyme reac,on to increase as temperature increases (un,l you start to denature the enzyme)? How does heat help? (1 pt) Heat helps in the way that it helps increase velocity and kineAc energy. The rate of an enzyme reacAon increases as temperature increases, causing the enzyme to denature. Because the molecules help reach the enzymes potenAal acAvaAon energy, in increases the rate of reacAon. 2
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