mbio-3400 practice lab exam 1

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Studocu is not sponsored or endorsed by any college or university MBIO 3400 506 Lab Exam 1 091715 Microbiology (Texas Tech University) Studocu is not sponsored or endorsed by any college or university MBIO 3400 506 Lab Exam 1 091715 Microbiology (Texas Tech University) Downloaded by Sidney Sapp (sidneysapp78@yahoo.com) lOMoARcPSD|22622403

Name: MBIO 3400 506 Lab Exam1 September 17, 2015 Q1. Why is Carbon considered an essental nutrient (macronutrient) for the growth of micro organisms? (1 pt) Q2. ___________________ is the most common solidifying agent used in media preparaton. (1 pt) Q3. Match the following: (2 pts) (a) Cocci (i) clusters (b) Bacilli (ii) spherical shape (c) Staphylo (iii) chains (d) Strepto (iv) rod shape Q4. In spore formaton, the stage in which DNA condenses and aligns itself in the center of the cell is called : (1 pt) (a) exosporangium (b) cortex (c) forespore (d) mother cell (e) vegetatve cell Downloaded by Sidney Sapp (sidneysapp78@yahoo.com) lOMoARcPSD|22622403

Name: Q5. During the spore formaton process, as calcium enters the spore (1 pt) (a) water follows it into the cell (b) glucose is removed from the cell (c) water is removed from the cell (d) it becomes a part of the cell membrane Q6. You are provided with a culture of Bacillus thuringensis and asked to perform Endospore staining. Afer fnishing the staining process, you observe the slide under the microscope. Vegetatve cells appear___________ in color, while spores appear_____________ in color. (2 pts) Q7. Spore have a tough outer covering made of the protein _______________________. (1 pt) Q8. What are parfocal objectves? (2 pts) Q9. The 40X objectve is also known as: (1 pt) (a) High- dry objectve (b) Oil- immersion objectve (c) Both of the above (d) None of the above Q10. ____________________________ is a system of lenses that usually magnify the primary image ten tmes. (1 pt) Downloaded by Sidney Sapp (sidneysapp78@yahoo.com) lOMoARcPSD|22622403

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Name: Q11. Label the parts of the microscope: (4 pts) Q12. Which technique is used to sterilize protein solutons? (1 pt) (a) Wet Sterilizaton (b) Dry Sterilizaton (c) Filtraton (d) Gaseous Sterilizaton Q13. The complete destructon of all viable organisms in a given environment to prevent contaminaton of an item is called _________________________. (1 pt) Downloaded by Sidney Sapp (sidneysapp78@yahoo.com) lOMoARcPSD|22622403

Name: Q14. You were asked to inoculate the bacteria Serrata marcescens aseptcally from a TSA plate to a TSA slant. Afer incubatng the TSA slant at 30°C for 24 hours, you notced that in additon to Serrata mascescens, other bacteria were also growing on the slant. Is this correct? If not, what do you think went wrong? Remember: Serrata marcescens has a red pigment when incubated at 30°C. (2 pts) Q15. List fve type of soil bacteria. Menton an example of any one type of bacteria. ( 3 pts) Q16. Afer performing serial dilutons for soil sample (10 grams), you took 0.1 ml from a 10 -6 diluton tube and spread it on a TSA plate. The plate was incubated at 30°C for a week. Afer this incubaton period, you counted 50 bacterial colonies/ CFUs on your TSA plate. How many bacteria were present in one gram of the inital sample. (2 pts) Q17. You were provided with Mycobacterium smegmats and Staphylococcus epidermidis cultures and asked to perform acid fast staining on their mixed smear. Afer the staining procedure, you observe the slide under a microscope. Mycobacterium smegmats cells appear______________ in color and Staphylococcus epidermidis cells appear ________________ in color. (2 pts) Q18. Match the following: (4 pts) (a) Schaefer-Fulton Stain (1) Loefer’s Methylene Blue (b) Ziehl-Neelsen Stain (2) Maneval's stain (c) Gram Stain (3) Spore Safranin (d) Capsule Stain (4) Gram Safranin Downloaded by Sidney Sapp (sidneysapp78@yahoo.com) lOMoARcPSD|22622403

Name: Q19. What is the primary stain used in the Endospore Staining staining method? (1 pt) Q20. What is the primary stain used in the Acid- Fast staining method? (1 pt) Q21. You were asked to expose TSA and PDA plates to air for 20 minutes. Afer 20 minutes, you put both the plates in 37°C incubator upside down. Is this procedure correct? If not, explain the mistake/ mistakes in the above procedure. (2 pts) Q22. List the type of micro organisms that grow on the following media plates: (3 pts) (a) Brain Heart Infusion Agar- ___________________________________ (b) Tryptcase Soy Agar - _______________________________________ (c) Potato Dextrose Agar- ______________________________________ Q23. The proper sequence of reagents in the Gram stain procedure is (2 pts) (a) iodine, ethanol, safranin, crystal violet. (b) safranin, crystal violet, ethanol, iodine. (c) crystal violet, ethanol, iodine, safranin. (d) crystal violet, iodine, ethanol, safranin. (e) ethanol, iodine, crystal violet, safranin. Downloaded by Sidney Sapp (sidneysapp78@yahoo.com) lOMoARcPSD|22622403

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Name: Q24. You were asked to perform gram staining of an unknown bacterial culture. Afer fnishing the staining process, you realize that you forgot to add the counter stain! What color do you expect to see when you observe the cells under the microscope? Please describe the possibilites for both G +ve and G -ve bacteria. (2 pts) Q25. ___________________________ are bacteria which do not stain or stain poorly using a Gram Staining procedure. (2 pts) Q26. ________________________ is completely dissolved by the decolorizer used in gram staining process. (1 pt) Q27. Why is heat fxing not performed during the process of capsule staining? (1 pt) Q28. List four functons of a bacterial capsule. (2 pts) Q29. During the smear preparaton for Capsule Stain, ________________________ dye is used instead of distlled water. (1 pt) Downloaded by Sidney Sapp (sidneysapp78@yahoo.com) lOMoARcPSD|22622403

Name: EXTRA CREDIT QUESTIONS 1. For a G +ve bacteria, why is the crystal violet- iodine complex not able to escape the cell afer the decolorizer has been applied? (2.5 pts) 2. Alternately, for a G -ve bacteria, why is the crystal violet- iodine complex able to escape the cell afer the decolorizer has been applied? (2.5 pts) Downloaded by Sidney Sapp (sidneysapp78@yahoo.com) lOMoARcPSD|22622403

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