Exam 1 Answers

.docx

School

Christopher Newport University *

*We aren’t endorsed by this school

Course

313

Subject

Biology

Date

Feb 20, 2024

Type

docx

Pages

7

Uploaded by reakins32

Exam 1 Answers 1. If a cell is waiting white it checks itself for DNA damage or misfiled dna proteins, it is likely at which cell cycle checkpoint? a. G2 checkpoint 2. This type of cell would be likely to have a haploid number of chromosomes a. gamete b. I chose somatic cell because I thought the option was different from the rest since all of them but somatic cell pertained to meiosis. Gamete is the right answer because since its apart of meiosis, it needs another gamete to produce a diploid state, meaning that the one gamete is in a haploid state. 3. From where does the chemical energy come to form a phosphodiester bond? a. the triphosphate group b. I selected the 3-OH group because I thought this was the location where the phosphodiester bonds would take place. The ENERGY comes from the triphosphate group creating the attachment. 4. A particular part of a chromosome has the sequence 5’-CGAGTCA-3’. What would be the complementary sequence on the other strand of the helix? a. 5’-TGACTCG-3’ 5. A restriction enzyme recognizes the following sequence and cuts at the arrow. What type of ends would the resulting molecules have? 5’-C|CTAGG-3’ a. Sticky 5’ overhang 6. Mitosis is technically complete following the division of the… a. nucleus
b. I chose the cytoplasm because I believed that the division of the cytoplasm would create the two different cells and completed mitosis. Nucleus is right because the division of the cytoplasm marks the end of cytokinesis, not mitosis since it had already ended prior. 7. What part of the DNA gives it a strong negative charge? a. Phosphate groups 8. What is the name of the enzyme that cuts cohesins in anaphase, initiating movement of the chromatids towards the poles? a. separase 9. In metaphase, spindle fibers attach directly to the… a. kinetochore b. I chose cohesins based solely off of guessing. Kinetochores is the right answer due to the spindle fibers connecting to these that allows for the movement of the chromosomes to the poles for mitosis to proceed. 10. A particular recognition sequence for a restriction enzyme contains six base pairs. Given the first three bases, complete the following palindromic recognition sequence. 5’- TAG___-3’ a. 5’-CTA-3’ 11. The Sanger method takes advantage of fluorescent, chemically-modified nucleotides to do its function. The ribose in these nucleotides have been modified to… a. Lack a hydroxyl group on carbon 3. 12. How would DNA replication be affected if the cell lost function of the helicase? a. replication would not be able to begin
13. This enzyme’s job is to make nicks in the DNA ahead of a replication fork in order to relieve supercoiling or over winding. a. Topoisomerase 14. Part of a gene contains the sequence 5’-GTATGCC-3’. What would be the sequence of the RNA that is transcribed from this DNA? a. 5’-GGCAUAC-3’ b. I chose 5’-CAUACGG-3’. My thoughts were going quite fast and did not remember I had to flip the sequence due to the orientation of the strand. Could have been an easy two points! 15. Erwin Chargaff studied nitrogenous base rations in a variety of species. If he found that 12% of bases in a hamster were adenine, what percentage of the bases would guanine complete? a. 38% 16. Aster spindle fibers are one of three classes of spindle fibers, which have a very specific job. What is the function of this type of spindle fiber? a. to anchor the central some to the cytoskeleton/cell membrane 17. Below is part of a chromosome that you would like to amplify using PCR. Which of the following would make a functional primer to aid in the PCR reaction? 5’-CCCTGGCTCTGTAAATGTTTCTAAGTG-3’ 3’-GGGACCGAGACATTTACAAAGATTCAC-5’ a. 5’-CACTT-3’ 18. What is the most important event that takes place during the S phase of the cell cycle? a. DNA replication
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
  • Access to all documents
  • Unlimited textbook solutions
  • 24/7 expert homework help