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Feb 20, 2024

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BIOL 1108L Heterotrophs in a Lake Ecosystem This assignment is designed to be completed and submitted at the end of your lab. This assignment should be saved as 1108L-HL-Lastname-Firstinitial.docx. It is your responsibility to ensure that you have answered every question and submitted the correct file. Today’s lab is on aquatic heterotrophs in the lake ecosystem. Read through Background Information: Aquatic Heterotrophs, Lab Activity: Examining Bacterial Plates, and Lab Activity: Examining Aquatic Heterotrophs to help answer the questions below. 1) Examine the plate streaked in the previous lab. Take note of the different types of bacteria on the plate. How many total bacteria are there? After applying the I 2 KI solution, is there evidence of the bacteria utilizing starch? (2 pts) 8 big ones 6 spiral/vineyard ones 70 small ones 22 whites ones # Bacteria Types Total Bacteria # Starch Use (Y/N/Partial) Bacterial Plate 4 106 Yes 2) Examine the prepared slides of aquatic heterotrophs Paramecium , Amoeba , and Stentor, at your bench. Also refer to the videos for each organism posted in eLC. Make a list of structural, physiological, and behavioral adaptations you notice for each organism: (1 pt for each organism) a. Amoeba Observations/Adaptations: This organism has a white cell full of some brown and purple spots on it. When focusing on it, I saw some yellow and pink colors. I could also see internal organelles. b. Paramecium Observations/Adaptations: The protozoa are shaped like little fishes. They had the colors green, purple, and pink. Some of them are oval shaped and some are more circular shaped. They have a coating surrounding them. I see some big circular spots inside it and some smaller ones. They are covered with cilia which helps allow them to move around. c. Stentor Observations/Adaptations: The stentor looks like big greenish kinda circular blue blobs and you can see the tails from some of them. The one I observed had green and blue spots inside the cell. I also observed that some of them had some sort of hair around them. 3) Examine the cultures of aquatic heterotrophs Dugesia and Cephalobus at your bench. Compare and contrast the movement and structural characteristics of these two benthic worms. (2 pts)
BIOL 1108L Heterotrophs in a Lake Ecosystem The Dugesia looked like a slug. It was slithering around and had two little black dots which are most likely his eyes. They were moving really slow but never staying still. It has a brownish olive color with dark dots all over the body too, it is skinny and long. Now the Cephalobus look like little tiny white worms which are moving and there are some brownish circles that look like little tiny seeds. The similarities between both though are they are skinny, long and both are mobile. Read Lab Activity: Scientific Writing Methods and the Scientific Article: Yağcılar & Yardımci Methods section. 4) What were the treatment groups and sample size for each group in this experimental design? (2 pts) A total of 150 gynandromorphic were randomly selected in this experimental design. Sample size is 50 Daphnia. They were sorted equally into 3 treatment groups, with 2 replications; 440 Hz, 432 Hz and control groups. 5) How was Daphnia heart rate data collected and analyzed? (2 pts) The heart rate was collected by recording and counting the beats per minute using a light microscope, stopwatch of 15 seconds, clicker for the heart beats and then multiplying by 4 for the bpm, and using a slide in a 7-day interval. The Daphnia was taken from the beaker and placed on a microscope from a pipette. 6) What other data was collected? (1 pt) Other data that was collected is the egg numbers and survival rates. 7) Describe the methods used in your Daphnia mini-experiment. How do your methods and results compare with others in the class? (3 pts) Methods: I placed a daphnia in a small dish and under a dissecting microscope. After I placed it in there I placed one drop containing the daphnia under the microscope. I then made to look at the heart to help count beats per minute. I used a stopwatch for 15 seconds and we used the clicker to count the heart beat within the 15 seconds. With my first observation I made sure to see the heartbeat without any variable and I counted 268 beats per minute. Next I add my variable (sucrose) into my well and just a drop so that my well doesn’t get overflown. Next I got my stopwatch ready and I counted the heartbeats per minute of the daphnia but with the variable (Sucrose) added into my well. With the Variable (sucrose) I counted beats per minute. Results:
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