Module+1+Information+Literacy+and+Guided+Investigation
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Module 1 – Information Literacy and Guided Investigation
The broad context of this first module is Experimental Design. Although we introduced this topic last term in Biology 1001A, many misunderstandings persist. During the icebreaker, your TA will revisit some of the concepts around experimental design and hypothesis testing. Your TA will also demonstrate how to use the GraphPad to run a t-test and generate a p-value. After the ice breaker, in groups of 4, you will have an opportunity to critique a scientific article (The Case of the Disappearing Teaspoons, link provided on OWL). Refer to the 3 questions provided during the session to complete the assignment and submit it on OWL during the session (one submission/group).
ASA1.4 Guidelines 1. Include your team number and the first and last names of all contributing team members at the top.
2. Include the questions in your submission; enter your answers (in a different font) below each question.
3. Use clear, concise, connected prose with standard punctuation and grammar.
4. Word count should not exceed 600, names and questions etc. included.
Specifications
To Pass, your Assignment must meet all of the following Specifications:
1. No more than 2 violations of Guidelines.
2. Score at least 4/6 marks.
3. Submitted as OWL Assignment before the deadline as specified by your session TA.
Fill up the group charter (posted on OWL) and email it to your group mates by the end of the session.
You will also conduct a guided investigation using live specimens in today’s session, so treat them gently. You will use a microscope to measure the pulsation rate of the live specimen.
Remember these rules of Microscopy from the fall term.
Always…
1. Use low power before medium or high power (use only low power in today's session).
2. Focus only with the fine adjustment knob. 3. Do not turn the fine adjustment knob more than 2 revolutions in either direction.
4. Adjust the light level if needed.
5. Clean lenses with the lens tissue only.
1
Table 1: Parts of microscope with function
Name
Function
Ocular eyepiece
Focuses image (usually 10X)
Objective lenses
Magnify elements, 4X, 10X, and 40X. The 4X lens is used to scan specimens to locate areas for more detailed viewing with the 10X and 40X lenses
Stage
Support and positions specimen
Stage motion control knobs
Permits movement along x and y axis to allow fine focusing of light on specimen
Coarse adjustment
Permits large upward and downward movement of objectives to bring specimen into range where it is subject to fine adjustment
Fine adjustment
Permits small upward and downward movement of objectives for fine focusing of image
2
Figure 1.
Basal pulsation rate of live specimen
– Daphnia magna
Anatomical features (will not be tested on SA or ASA or any test)
Daphnia
magna
or the water flea
(Fig.1), is a tiny freshwater crustacean that is common in most
lakes and ponds. It is a filter-feeder that primarily consumes phytoplankton and possesses a transparent carapace, which makes it an ideal organism for the study of basic physiology.
Figure 1. General anatomical features of Daphnia (40X)
Daphnia
is a laterally compressed crustacean in which the body is partially surrounded by a bivalve carapace which encloses the trunk of the animal, but not the head, and terminates posteriorly in an apical spine. The head projects ventrally and somewhat posteriorly as a short beak, so the body looks like a plump bird. The head bears the minute first antenna bristling with chemoreceptors, and very last second antennae, which are locomotor organs. The down stroke of the second antennae propels the 3
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