Lab 15 Bacterial Unknowns FA23

Activity 2. Media Used in Isolating Coliforms You are a group of microbiologists tasked to test the presence of coliforms in the newly built water station. You are asked to isolate E. coli, Salmonella, and Shigella. Enumerate the media used for each step of isolating these bacteria. Include pictures and references. Bacteria Enrichment (Broth/Agar) Presumptive test (Broth/Agar) Isolation Media (Broth/Agar) E. coli Salmonella Shigella 1. How can you confirm that E. coli, Salmonella, and Shigella are present in the newly built water station? Explain. 2. What is the difference between nutrient broth and nutrient agar? 3. What is the importance of these steps (Enrichment, Isolation, and presumptive test) in isolating bacteria? Conclusion about the process of isolating bacteria

Instruction: answers must be in numbers. You are culturing bacteria using a petri dish, the bacteria grow very well on this plate you have. However, your microbiology instructor wants to know how many bacteria per milliliter (bacteria/mL) are on your plate. And as you have remembered during the first day of inoculating this bacteria you used a imL aliquots sample of a 1:10,000 dilution, and the total colony you counted on this plate is 170. How many bacterial per milliliters would that be?

General Instructions:Choose 1 bacteria/diseases and fill out the details. An example of an answered template is also provided for your reference.    Causative Agent and Disease Profile for S. aureus Template and Example   ITEM MSM PROFILE   MICROBIAL PROFILE   I MICROORGANISM/CAUSATIVE AGENT Staphylococcus aureus A GRAM REACTION (+) B OXYGEN REQUIREMENT Facultative Aerobes C SIZE 1.5 µm D SHAPE Cocci in clusters E HABITAT Normal flora of skin/anterior nares/pharynx F DISCOVERY   G MICROSCOPIC IMAGE   II DISEASE PROFILE Scalded skin syndrome A DISEASE/S                        Skin and Wound Infections       Scalded Skin Syndrome Toxic Shock Syndrome Food Poisoning Pneumonia B SYMPTOMS OF THE DISEASE A high fever · Nausea and vomiting · A rash on your palms and soles that resembles a sunburn C INCUBATION PERIOD 2 and 4 hours (range 30 minutes to 8 hours) D MODE OF…

List each step of learning goals that was listed from the picture

Please match the test with its description to assess your understanding of the kinds of data collected during information gathering and your understanding of the processes involved in identifying microbes from samples. 1. using macroscopic and microscopic traits for identification appearance 2. tests that determine chemical characteristics including enzyme production and nutritional requirements of the microbe biochemical tests 3. analyzing the genotype of an organism DNA profiles 4. tests the organism against known antibodies to determine if there is a reaction between the organism and the antibody immunologic testing v

webapps/assessment/take/launch.jsp?course_assessment_id%3D 498749 1&course_id3 111786 1&content id=D Customize Links Free Hotmail Windows Windows Media Imported From IE Importe Serial dilution QUESTION 2 What type of agar is TSA? (select all that apply) Nutrient

I need help with microbiology/2010 1. Describe where the following items should be discarded: a) gloves b) petri dishes   c) test tubes   d) microscope slides   2. Describe the safety procedures for the following hypothetical situations: a. You spilled a full test tube of bacterial culture on the bench top.   b. You notice flames coming from the tubing of your Bunsen burner

LABORATORY REPORT: Aseptic Technique & Inoculation of Bacteria Guide Questions. 1. Why is direct flaming preferred when disinfecting loops and needles? 2. Why is it important to flame the entirety of the loop and not just the tip? What consequences can be seen when this process is not done correctly? 3. What is the difference between quadrant streak method A from method B? 4. Why do we use a pencil instead of a pen when labelling culture tubes or plates? Are there other alternatives in labelling? 5. Why is an inoculating needle used in Subculturing Techniques? Can a loop be used instead? NOTE: Please try to answer all of the question asked, i promised to give you a good ratings

Questions: 1. What happens to the number of bacteria as you streak from one sector to another? 2. What happens to the colonies when the bacteria are separated well? 3. How bacteria does each colony come from? many 4. Why do you make sure that the inoculating loop is red hot? 5. What happens when the streaking is not correct? 6. When do you flame the mouth of the cultures tubes? 7. Why you should not use the inoculating loop when it is red hot?

Please answer and explain. Provide references if possible: TOPIC: PARASITES: HARADA-MORI CULTURE TECHNIQUE 1.) What safety measures must be observed in handling the Harada-Mori Culture Technique preparation? Why? Explain.

Guide Questions: Define and contrast the terms “sterile” and “clean”. List 5 other methods of sterilization and describe the principle/s and when each method is used. What are the requirements to support microbial growth?

For laboratory exercise of Antibiotic susceptibility test in disk-diffusion method. What does an ‘I’ result mean in disk diffusion susceptibility test?

all three please

Hello, please read the attached Microbiology question and answer the question correctly. Please aim for about 3-4 sentences max (enough to fit within the lines given). Write in complete sentences (No Bullet Points).  *If you correctly answer the question, I will provide a Thumbs Up to you. Thank you.

Question:- How to perform a bactericidal test bactericidal test of Dental Pulp Stem Cell (DPSC)?

OBJECTIVES: 1. To understand how to perform the bacterial endotoxins test using gel-clot technique on a parenteral preparation. 2. To understand the importance of the preparatory testing3. To interpret test results and provide disposition for the bacterial endotoxins test based on current USP requirements. Question: 2. The incubator is exposed to mechanical vibrations.Effect:Rationale:

Complete both pages correctly

Need help with the following questions for microbiology 1. Which of the following total magnification (on a compound light microscope) must be used to identify bacterial morphology A. 100 x B. 400 x C. 300 x D. 1000 x 2. You learned about the Bio Safety Level (BSL) categories in Microbiology Lab, which of the following BSL category is safe to processed in a lab on open counter top? A. BSL3 B. BSL2 C. BSL1 D. BSL4 E. BSL1 and BSL2 3. Streak Plate method is used for A. all choices are correct B. used to isolate bacteria from a mixed culture/sample C. is used routinely in Microbiological labs D. is essential tool for isolation and identification of any specific bacteria 4. A selective laboratory growth medium for Gram negative bacteria will allow -------. A. only algae to grow B. only viruses to grow C. only G+ to grow D. only G- to grow 5. You learned that microbes are ubiquitous and they are part of normal flora of various body sites, however which body sites is/are supposed to be…

need typed answer asap

Discusses the methods to -1 optimise fluorescent cellular (staining (priority first List the freezing -2 parameters of the slow cooling technique Parameter of verification -3 precedure of freezing 9

Need ASAP. Please answer all the items in the table. Thank you!

Give the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions Picture   1. Microscope     2. Colony Counter     3. Autoclave     4. Microbiology incubator     5. Drying oven     6. Refrigerator (microbiology)     7. Bunsen burner/alcohol lamp     8. Candle jar     9. Anaerobic jar     10. Microhood or Bacteriologic hood/Safety hood/Safety cabinet

Case Study: Lab Errors

INSTRUCTIONS: Please do not copy here in Bartleby or in Google. QUESTIONS : 1. What is the importance of the aseptic technique? Explain.

Expert I just need sources with citations.  Question:- Describe the mode of action beta-lactam, aminoglycosides, polyenes, and quinolone antimicrobial agents on bacterial cells. Provide 1 or 2 sources with the citations in APA format

MICROBIOLOGY:  Microscopic Morphology of Microbes Write your introduction (This includes principles, significance of the study, objectives of the experiment and how the objectives were achieved. This part must also be in the passive voice and past tense. Introduction must be short but packed with relevant content). Just answer objectives and  principles.

Instructions: Species name 1: Sample illustration: Taxonomic account (domain to species): At least two phenotypic methods used in its identification (Describe concisely). At least two genotypic methods used in its identification (Describe concisely). References: MICROBE TAXONOMIC ACCOUNT Choose a microorganism from among the microbial groups. Fill in the information required for your chosen microbe.

A Photo credit: BSP3D- Group 5 Pharm. Bio. Sci 3 Laboratory (S.Y. 2016-2017) Sample is Bacillus subtilis; A-Ciprofloxacin; B-Ampicillin; C-Tetracycline; D- Vancomycin Refer to the image above and answer the following questions: 1. By just looking at the example above, which do you think is the most effective antibiotic for Bacillus subtilis? which is the least effective? and why? 2. How will you measure the zone of inhibition of vancomycin (letter D)? 3. What is the importance of evenly distributing the microbe onto the plate? 3. Subtilis

Hello, please read the attached Microbiology question and answer the question correctly. Please aim for about 3-4 sentences max (enough to fit within the lines given) *If you correctly answer the question, I will provide a Thumbs Up to you. Thank you.