Post-lab for lab #4

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Feb 20, 2024

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Post-lab for lab #4 Rabbit psoas muscle experiment Can a muscle contract without expenditure of ATP? No, muscles can’t contract without ATP, muscle contraction needs at least some level of ATP. The ATP is critical as it is the energy for a contraction to happen. If the muscle does not have enough ATP, depending on if it's in the middle of a contraction, it can become stuck in its contracted state. Or it can just be stuck in its relaxed state if it had not contracted yet. Did any of your clumps of fibers contract and remain in a contracted state for about an hour? If so, what may account for that in terms of the availability of ATP? Looking at our data, no, our muscle fibers did not remain contracted as its length increased compared to the measurements after the 45 seconds. The increase in length meant that the muscles relaxed as opposed to it contracting. If it stayed contracted that would mean that there wasn’t any ATP as contracted muscles without ATP would remain in their contracted state and the length after 1 hr would have been the same as the 45 sec measurement. Since it relaxed that meant that there was enough ATP to cause the muscle to relax out of its contracted state. However after the 1 hr and when we added the dropper, it gave the muscle fibers enough ATP to contract once again. At start After 45 seconds ATP Only __________21__mm____ ATP Only _____20mm___________ Minerals Only _______21__mm____ Minerals Only __21 mm_________ ATP + Minerals ______22___mm___ Minerals + ATP. 22 mm________\ One hour later Any change from the first 45” length? ATP Only _________21__mm____ ATP Only _______got longer________ Minerals Only _______23___mm___ Minerals Only ______got longer______ ATP + Minerals _____23___mm____ Minerals + ATP ______stayed the same______ After dropper 45 seconds ATP Only ______20___mm_______ Minerals Only ____21__mm_______ ATP + Minerals _____22 mm_______
Muscle tone and isotonic (different) /isometric (stays the same) contractions (MY DATA) 1. Both arms extended directly in front of the body (parallel to the floor).29 cm 2. Both arms flexed with palms facing upward (forearms parallel to the floor). 30 1/2 cm 3. Right arm extended and grasping a heavy textbook (parallel to the floor). 29 1/4 cm 4. Left arm extended and grasping a heavy textbook (parallel to the floor). 29 cm 5. Right arm flexed and grasping a heavy textbook (forearms parallel to the floor). 31 1/2 cm 6. Left arm flexed and grasping a heavy textbook (forearms parallel to the floor). 32 cm 7. Attempting to lift the black top of your table. 30 3/4 cm Indicate which activities were isotonic and which were isometric. The activities that were isometric were lifting the heavy textbook and having your arm extended. It exceeds muscle peak so that is why the muscle is not seen to be shortening. The muscle diameter still stayed fairly the same as when I was not holding the heavy textbook. The isotonic actions were having your arms flexed while grasping the heavy textbook because it overcame the resistance allowing for the muscle to shorten. Comparing the diameters when holding the textbook flexed versus not holding the textbook flexed it seemed that carrying a load changed the diameter of my bicep. Also when I was lifting the black top it also increased the diameter of my bicep as well. EMG and muscle fatigue- Name two sources of electrical activity measured via EMG. Neuron stimulation and muscle contraction Which entry or entries in your charts for fist clenches measure(s) only tone? The background activity where we are not clenching and weakest clench in the rested column were the entries that measured tone because they had the lowest level of contractile activity in a rested muscle. Explain what caused the increase in mV as fist clench intensified . The increase in mV as fist clenching intensified was caused by an increase of calcium ions entering through the voltage gated calcium channels. The increase of calcium increased the levels of glutamate release. Glutamate causes EPSP which opens sodium channels causing the depolarization of Vm. Glutamate is also increased by physical exercise, which was happening as the fist clenching intensified (flexing of the fingers). As the strength increases depolarization is occurring causing the increase in mV (the Vm closer to zero).
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