MCB2010C_Lab4Report
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Tallahassee Community College *
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Course
2010
Subject
Biology
Date
Feb 20, 2024
Type
docx
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3
Uploaded by ChiefPonyPerson175
Name(s)
: Nicole Linebaugh
Section: 2360 Lec/Lab
LAB 4 REPORT
: BASIC BACTERIAL CULTURING METHODS & ISOLATION OF
PURE BACTERIAL CULTURES & PREPARING A STREAK PLATE & PREPARING
A SPREAD PLATE
1.
Evaluation of streak
plate: Attach at least 1 digital image of your streak plate indicating the distribution of the colonies. Is there evidence of contamination among the colonies on
your streak plate? No
Are you satisfied with your streak plate? No
If not, how can you improve your streak-plate technique? I could improve my streak plate by remembering to
sterilize an inoculating loop or needle. 2.
3.
Evaluation of spread
plate: Attach at least 1 digital image of your spread plate indicating the distribution of the colonies. Is there evidence of contamination among the colonies on
your spread plate? No
Are you satisfied with your spread plate? Yes. If not, how can you
improve your spread-plate technique?
4.
Compare the results of your streak and spread plates. Which method achieved the best separation of colonies? The results of my streak plate had more colonies and microbes growing than my spread plate. The method to achieve the best separation of colonies would be the streak plate because, in the streak plate, the colonies are diluted progressively and spread apart with each streak for more isolation. 5.
In regard to bacterial growth on solid media, define the term “colony” scientifically.
“A colony is a population of cells arising from a single cell or spore from a group of attached cells; Often called a colony-forming unit (CFU).” Tortora, Funke & Case. Microbiology: An Introduction, 13th Ed., 2019: Pearson.
6.
Characteristic features of bacterial growth in culture can be used for differentiation of bacterial species. Describe your colonies appeared on your streak plate:
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Related Questions
BONUS (15 points)
The fallowing series of dilution was prepared from a specimen to determine the number of bacteria.
There were 62 colonies on the agar plate prepared by transferring 0.3 ml from the tube number 4.
Calculate the dilution factors for each tube.
What is the cell concentration in the original specimen?
Calculate the total number of cells in tube number 2.
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A) F12
F9
F10
F7
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5
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T
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J
K
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I am wondering whether E. coli can be used as spiked in sample for Bacteria vaginosis detection through qPCR? Please help me with the information including how to purchase it.
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The first 3 questions have been answered already. Do not need to answer the first 3 questions please
Thank you
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d-6f06-490d-b3d3-cb18767c100
Post-Lab: Streak Plate Isolation
Homework. Due in 12 hours
2/5 answered
Unanswered
Post-Lab: PIV 2.5
Homework Unanswered
How do colonies of E. coli differ from those of P. mirabilis?
inancwarnd
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t/Root/Pages/TestTaker/MonitorLaunch.aspx?id%3DE9B80AE3-962E-4628-B79D-A3F06F1CAB72
MonitorLaunchaspx?id%3DE9B80AE3-962E-4628-879D-A3F0EFICABT2-Google Chrone
uninoculated
tube
The medium shown here was inoculated with a bacterium then incubated for 48 hours; an uninoculated control tube is also
present for comparison. This particular bacterium gave a
for this test.
a) O negative result
b) O positive result
c) O indeterminate result
Review Later
d) O variable result
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Write the process/steps in Preparation of Bacillus atrophaeus culture using Blood agar plates and MacConkey agar plates.
Note: This will perform the students so please write it in the easiest manner. 5-10 sentences only Thanks
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Highlighted parts
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As a medical microbiologist, if you are presented with a patient suspected to have a bacterial disease, describe the various steps you would follow, to establish a laboratory diagnosis.
Please keep brief - 8 sentences/dot points max.
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Please I want the gram negative identification, gram stain reaction, cell shape, cell arrangement,test,results , genus and species
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Please Help
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Topic: Spread-Plate Method
The bacterial suspension was not spread evenly on the surface of the agar.Effect: Rationale:
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Hii, can anyone help me with this. Your help would be very much appreciated, thank youuu.
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Please answer all the items. Will give you thumbs up and good ratings.
Choices:
Gram stain
Simple stain
Ziehl-neelsen method
Schaeffer- Fulton method
Negative stain
None of the choices
1. Staphylococcus aureus cells turn purple while Neisseria gonorrhoeae become pink.
2. Bacterial flagella becomes visible *
3. Highlights the presence of a certain cellular structure produced by species in the genera Bacillus and Clostridium.
4. Background is dark, cells unstained or stained with simple staining
Distinguishes the genera
5. Mycobacterium and Nocardia from other bacteria .
6. Pink to red acid-fast cells and blue non-acid-fast cells .
7. Reveals size, morphology, and arrangement of cells .
8. This is a required question
Green endospores and pink to red vegetative cells .
9.Differentiates a thick cell walled bacteria from a thinner but more complex one
10. Reveals the bacterial capsules *
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I need help
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Write the process/steps in Preparation of Bacillus atrophaeus culture using Blood agar plates and MacConkey agar plates.
Note: This will perform the students so please write it in the easiest manner. Thanks
arrow_forward
I need help with a micobiology question, Explain why adding 1 ml of a phage sample into a 9 ml tube of sterile saline creates a 1:10 dilution?
thank you
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Write short note or summary on the given topics:
1. Co acervation method of microencapsulation
please answer at your own words
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MEDICALLY SIGNIFICANT VIRUSES (answer the following and complete the boxes of the
virus)
• Retrovirus
ITEM
1
A
C
D
E
F
B OXYGEN REQUIREMENT
G
||
A
B
C
D
E
F
G
H
MICROBIAL PROFILE
MICROORGANISM/CAUSATIVE
AGENT
GRAM REACTION
SIZE
SHAPE
HABITAT
DISCOVERY
MSM
MICROSCOPIC IMAGE
DISEASE PROFILE
DISEASE/S
SYMPTOMS OF THE DISEASE
INCUBATION PERIOD
MODE OF TRANSMISSION
DIAGNOSIS
TREATMENT
PREVENTION
NO OF DAYS BEING SYMPTOMATIC
PROFILE
Retrovirus
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66671/take/questions/800382
Regarding Gram staining, what will be the appearance of the Gram-negative bacteria
if...
All steps are done correctly?
[Choose ]
[Choose ]
Pink
The slide is not heat-fixed prior to Purple
staining?
Clear
Orange
Crystal violet was omitted?
lodine was omitted?
Only iodine was used?
Acetone:alcohol was omitted?
Too much acetone:alcohol was
used or was left on for too long?
Not enough acetone:alcohol was
used?
Carbol fuschin was omitted?
Carbol fuschin or safranin was
omitted?
[Choose ]
[Choose ]
[Choose ]
[Choose ]
[Choose ]
[Choose ]
[Choose ]
[Choose ]
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2. Calculate the effective dilution. You were given a sample which contained 5.5mL of
bacterial culture. You added 10uL of phage solution to the culture. What was the
effective dilution produced by the addition of the phage solution?
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Can you help me?
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Write the Preparation of Bacillus atrophaeus culture using Blood agar plates and MacConkey agar plates (in a past tense sentences manner).
Note: This will perform the students so please write it in the easiest manner. 5-10 sentences only Thanks
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Please help me
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Question:-
Describe control strains used in the clinical microbiology laboratory and explain their maintenance in the laboratory. ( write BY WORD and all steps I need).
Introduction
Discussion
References
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In not more than 7 sentences, discuss briefly the techniques used in microscopic examination of stool.
Long answers are very much appreciated. State your answer in your own words and do not plagiarize. Thanks in advance!
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I need help with my homework
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https://youtu.be/w7aIxiZQ60g
Multiplexing agglutination https://youtu.be/uWStmyJ5Qc0
This is the multiplexing agglutination. Lab report I don’t really know what to talk about, the data, conclusions and the purpose of this. Need help please
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Related Questions
- BONUS (15 points) The fallowing series of dilution was prepared from a specimen to determine the number of bacteria. There were 62 colonies on the agar plate prepared by transferring 0.3 ml from the tube number 4. Calculate the dilution factors for each tube. What is the cell concentration in the original specimen? Calculate the total number of cells in tube number 2. étv A) F12 F9 F10 F7 % & %3D delete 5 { T Y J Karrow_forwardI am wondering whether E. coli can be used as spiked in sample for Bacteria vaginosis detection through qPCR? Please help me with the information including how to purchase it.arrow_forwardThe first 3 questions have been answered already. Do not need to answer the first 3 questions please Thank youarrow_forward
- d-6f06-490d-b3d3-cb18767c100 Post-Lab: Streak Plate Isolation Homework. Due in 12 hours 2/5 answered Unanswered Post-Lab: PIV 2.5 Homework Unanswered How do colonies of E. coli differ from those of P. mirabilis? inancwarndarrow_forwardt/Root/Pages/TestTaker/MonitorLaunch.aspx?id%3DE9B80AE3-962E-4628-B79D-A3F06F1CAB72 MonitorLaunchaspx?id%3DE9B80AE3-962E-4628-879D-A3F0EFICABT2-Google Chrone uninoculated tube The medium shown here was inoculated with a bacterium then incubated for 48 hours; an uninoculated control tube is also present for comparison. This particular bacterium gave a for this test. a) O negative result b) O positive result c) O indeterminate result Review Later d) O variable resultarrow_forwardWrite the process/steps in Preparation of Bacillus atrophaeus culture using Blood agar plates and MacConkey agar plates. Note: This will perform the students so please write it in the easiest manner. 5-10 sentences only Thanksarrow_forward
- Highlighted partsarrow_forwardAs a medical microbiologist, if you are presented with a patient suspected to have a bacterial disease, describe the various steps you would follow, to establish a laboratory diagnosis. Please keep brief - 8 sentences/dot points max.arrow_forwardPlease I want the gram negative identification, gram stain reaction, cell shape, cell arrangement,test,results , genus and speciesarrow_forward
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