MCB2010C_Lab4Report

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Tallahassee Community College *

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2010

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Biology

Date

Feb 20, 2024

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docx

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3

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Name(s) : Nicole Linebaugh Section: 2360 Lec/Lab LAB 4 REPORT : BASIC BACTERIAL CULTURING METHODS & ISOLATION OF PURE BACTERIAL CULTURES & PREPARING A STREAK PLATE & PREPARING A SPREAD PLATE 1. Evaluation of streak plate: Attach at least 1 digital image of your streak plate indicating the distribution of the colonies. Is there evidence of contamination among the colonies on your streak plate? No Are you satisfied with your streak plate? No If not, how can you improve your streak-plate technique? I could improve my streak plate by remembering to sterilize an inoculating loop or needle. 2. 3. Evaluation of spread plate: Attach at least 1 digital image of your spread plate indicating the distribution of the colonies. Is there evidence of contamination among the colonies on your spread plate? No Are you satisfied with your spread plate? Yes. If not, how can you
improve your spread-plate technique? 4. Compare the results of your streak and spread plates. Which method achieved the best separation of colonies? The results of my streak plate had more colonies and microbes growing than my spread plate. The method to achieve the best separation of colonies would be the streak plate because, in the streak plate, the colonies are diluted progressively and spread apart with each streak for more isolation. 5. In regard to bacterial growth on solid media, define the term “colony” scientifically. “A colony is a population of cells arising from a single cell or spore from a group of attached cells; Often called a colony-forming unit (CFU).” Tortora, Funke & Case. Microbiology: An Introduction, 13th Ed., 2019: Pearson. 6. Characteristic features of bacterial growth in culture can be used for differentiation of bacterial species. Describe your colonies appeared on your streak plate:
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