Module_6 UEG Peterman

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Genomics Education Partnership Last Update: 08/20/2023 1 Module 6: Alternative Splicing Answer Sheet Q1. Given that exons are shown by the black boxes, and introns are shown by thin lines with arrowheads in the FlyBase Genes track, what does this tell us about the first intron of tra-RB compared to that of tra-RA? Q2. Given what you know about the initiation of translation, which of the 3 possible reading frames is used for both the tra-RA and tra-RB products? Q3. Give the coordinate for the last base of the first exon for tra-RA. Q4. Give the coordinate for the last base of the first exon for tra-RB. Q5. What is the consensus sequence for the 5’ splice site (donor site) ? Q6. What are the coordinates for the 5’ splice site in tra -RA? Q7. What are the coordinates for the 5’ splice site in tra -RB? Q8. What is the phase at this splice site? Q9. What are the coordinates for the first base of the second exon in tra-RB? Q10. What is the consensus sequence for the 3’ splice site?
Genomics Education Partnership Last Update: 08/20/2023 2 Q11. Wh at are the coordinates for the 3’ splice site in intron 1 of tra -RB? Q12. What phase do we anticipate? Q13. Given this, what is the reading frame for tra-RB exon2? Q14. Does this make sense, given the location of stop codons? Q15. What are the coordinates for the first base of the second exon in tra-RA? Q16. What is the consensus sequence for the 3’ splice site? Q17. What are the coordinates for that sequence in intron 1 of tra-RA? Q18. Given the phase at the donor site, what phase are we looking for here? Q19. Given this, what is the reading frame for tra-RA exon 2? Q20. Does this make sense, given the location of stop codons? Q21. Look back at Module 5, Q17 . Using the coordinates you’ve written in your answer to that question, calculate the number of amino acids in the protein translated from tra-RA. Do this by calculating the size of each exon, added the sizes of all exons together, and dividing by 3 (the number of bases in a codon). ______ Q22. Write down the coordinates for exon 1.
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