Module_6 UEG Peterman
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Genomics Education Partnership Last Update: 08/20/2023 1 Module 6: Alternative Splicing
Answer Sheet
Q1. Given that exons are shown by the black boxes, and introns are shown by thin lines with arrowheads in the FlyBase Genes track, what does this tell us about the first intron of tra-RB compared to that of tra-RA?
Q2.
Given what you know about the initiation of translation, which of the 3 possible reading frames is used for both the tra-RA and tra-RB products? Q3.
Give the coordinate for the last base of the first exon for tra-RA. Q4.
Give the coordinate for the last base of the first exon for tra-RB. Q5.
What is the consensus sequence for the 5’ splice site (donor site)
? Q6.
What are the coordinates for the 5’ splice site in tra
-RA? Q7.
What are the coordinates for the 5’ splice site in tra
-RB? Q8.
What is the phase at this splice site? Q9.
What are the coordinates for the first base of the second exon in tra-RB? Q10.
What is the consensus sequence for the 3’ splice site?
Genomics Education Partnership Last Update: 08/20/2023 2 Q11.
Wh
at are the coordinates for the 3’ splice site in intron 1 of tra
-RB? Q12.
What phase do we anticipate? Q13.
Given this, what is the reading frame for tra-RB exon2? Q14.
Does this make sense, given the location of stop codons? Q15.
What are the coordinates for the first base of the second exon in tra-RA? Q16.
What is the consensus sequence for the 3’ splice site?
Q17.
What are the coordinates for that sequence in intron 1 of tra-RA? Q18.
Given the phase at the donor site, what phase are we looking for here? Q19.
Given this, what is the reading frame for tra-RA exon 2? Q20.
Does this make sense, given the location of stop codons? Q21.
Look back at Module 5, Q17
. Using the coordinates you’ve written in your answer to that question, calculate the number of amino acids in the protein translated from tra-RA. Do this by calculating the size of each exon, added the sizes of all exons together, and dividing by 3 (the number of bases in a codon). ______ Q22. Write down the coordinates for exon 1.
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RNA Transcription, Translation, and Mutation
Worksheet
First, here is a strand of DNA. This strand contains both a gene and its promoter region. Circle
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TATATATATTACGTTGCATACGCTCAACGGTCGAAACTGCATGGGCAC
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1)
2)
3)
Using a codon chart of your choice (one can be found here, or here) translate the above RNA
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acids in the image below:
Now imagine that a mutation took place in the original strand of DNA (marked in red)
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FALSE
Unanswered
a Save
58
What segments of the pre mRNA comprise the coding region?
pre-mRNA
5' UTR
Exon
Intron
Exon
Intron
Exon
3' UTR
MRNA
Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer.
a
5'UTR-EXONS-INTRONS 3'UTR
5'UTR-EXONS-3'UTR
5'UTR-INTRONS-3'UTR
P.
EXONS
a Save
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What is b?
3'
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You continue to study the expression of the hexose kinase gene and capture the following electron
micrograph of the gene being expressed.
MRNA
1
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ORI
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Biol 1406-Lec 17 - Gene Exp X
acconline.austincc.edu/ultra/courses/_891351_1/cl/outline
Question completion Status.
Blackboard Learn
Which of the following stater X
9.
Which of the following statements about transcription is not true?
In both prokaryotes and eukaryotes, pre-mRNA is modified after transcription by adding a 5' cap and a 3' poly-A tail and splicing out introns leaving coding exons.
Paraphrasing Tool - QuillBot
Your disk is almos
Save space by optir
O
During transcription only one DNA strand called the template strand is read and rewritten by RNA polymerase with the template strand read in the 3' to 5' direction and the mRNA
transcript synthesized in the 5' to 3' direction.
O
During initiation of transcription, RNA polymerase binds to the promoter region in the DNA, unwinds the DNA and binds together RNA nucleotides complementary to the template
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O
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4. What human disease has been connected to this gene?
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