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Oct 30, 2023

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Anatomical Barriers as Ecosystems Symbiotic relationship b/w a host & microbe: Mutualism : both partners benefit ● Ie: bacteria in large intestine synthesize vitamin K & B vitamins that are available for body to absorb Commensalism : one partner benefits while the other is unaffected ● Many microbes living on the skin are neither harmful nor helpful to human host; obtain food & other necessities from the host Parasitism : parasite benefits at the expense of the host The Human Microbiome Normal microbiota : group of microorganisms routinely found growing on the body of healthy individuals Microbiome = total genetic information of the community Resident microbiota = inhabit body sites for extended periods Transient microbiota = temporary occupants Colonization by normal flora → delivery, handling, env’t, food; Δ’s over time; body region specific Composition of the Microbiome Firmicutes & bacteroidetes are commonly represented phyla in the intestinal tract of adults Beneficial Roles Protects against infection by excluding pathogens, priming the adaptive immune system to react against pathogens, helping the immune system to develop immune tolerance to harmless substances, aiding digestion, & producing certain vitamins Principles of Infectious Disease Infection : establishment & proliferation of a microbe on or w/in a host; parasitic relationship w/the host Member of the normal microbiota colonized host; pathogen is having either colonized or infected host Disease : harmful alteration of host tissues or metabolic activities Infectious Disease : infection results in disease Symptoms = subjective effects (pain & nausea) Signs = objective evidence (rash, pus formation, swelling) Pathogenicity (ability to produce a disease) Primary pathogen : causes disease in otherwise healthy individuals ● Ie: malaria, measles, influenza, strep throat, plague, tetanus, tuberculosis Opportunistic pathogen : causes disease ONLY when the body’s innate or adaptive defenses are compromised, or when introduced into an unusual location ● Can be members of normal microbiota or from env’t → immunocompromised ● Ie: Pseudomonas = common env’t bacteria that comes into contact w/healthy individuals w/o harmful effect, but causes fatal infections w/genetic disease cystic fibrosis & burn patients Virulence : traits of a microbe that promote pathogenicity → How dangerous a bacteria is Determined by ID 50 (# of organisms to establish infection) & LD 50 (amt of toxin needed to be lethal) Some pathogens = highly virulent; few bacteria can cause disease ● Ie: Streptococcus pyogenes causes strep throat, but certain strains are particularly virulent, causing disease like necrotizing fasciitis Virulence factors : traits of a microorganism that specifically allow it to cause disease → genes encoding these traits can sometimes be transferred horizontally Infectivity = ability to enter host & reproduce Invasiveness = ability to spread Pathogenic potential = ability to do damage
Characteristics of Infectious Disease Communicable/Contagious diseases : spread from one host to another (colds & measles) Infectious dose : # of microbes necessary to establish an infection ○ ID 50 = # of microbial cells administered that resulted in disease in 50% of test population Progression = incubation period, illness, & convalescence ● During illness = signs & symptoms of the disease ○ Prodromal phase = period of early, vague symptoms (headache) Duration = according to timing & duration of symptoms Acute : symptoms that develop quickly, last short time; strep throat Chronic : develop slowly, last for months/years; tuberculosis Latent : never completely eliminated; microbe continues to exist in host tissues; held in check by immune system w/o causing symptoms; genome hides in sensory nerves Ie: chickenpox → shingles; causative agent becomes latent = tuberculosis, cold sores, genital herpes Determining cause of an infectious disease Koch’s Postulates : provide foundation for determining the cause of infectious disease An organism suspected of causing the disease must be able to grow in a laboratory medium Molecular Koch’s Postulates : used to identify the virulence factors responsible for disease The virulence factor gene or its product should be found in pathogenic strains of the microorganism Mutating the virulence gene to disrupt its function should reduce the virulence of the pathogen Reversion of the mutated virulence gene or replacement w/a wild- type version should restore virulence to the strain Koch’s = used to establish that a given microbe causes a specific infectious disease. Molecular = used to identify the virulence factors responsible for disease. Both remind us that scientific evidence should be foundation for determining cause of disease
Establishing Infection: Entry into the Host Microbial Adherence Bacteria use adhesins to bind to host cells Colonization : microorganism must multiply in order to colonize host; pathogens grow in biofilms Rapid turnover of pili, antigenic variation, & IgA proteases allow bacteria to avoid the effects of secretory IgA Entry into Host: Invasion–Breaching Anatomical Barriers Penetrating the skin Bacteria invade relying on skin-damaging injury (ie: Staphylococcus aureus enters tissues via cut/wound & Yersinia pestis is injected by infected fleas) Penetrating mucous membranes Some pathogens induce mucosal epithelial cells to engulf bacterial cells (directed uptake) & exploiting antigen-sampling processes Directed Uptake: Endocytosis (Delivering effector proteins to host cells → induce phagocytosis; type III secretion system of Gr-) Antigen-Sampling Processes: ● Shigella species use M cells to move across the intestinal epithelial barrier. Once the bacterial cells are on the other side, macrophages ingest them, but the bacteria are able to escape & then infect other cells
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