151-3 disc-22fa HW4

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151-3 Introductory Biology - Discussion Homework 4 Week of October 3, 2022 Name: Katie Scheer Reminder: Submit your homework on Canvas by the start of your scheduled discussion section! Please use a different font color so that your answers stand out. 1. The summary formula for cellular respiration is C 6 H 12 O 6 + 6 O 2 → 6 CO 2 + 6 H 2 O + Energy a. Where is each of the reactants below used in respiration (glycolysis, the conversion of pyruvate to acetyl-CoA, Krebs cycle, or Oxidative phosphorylation)? b. Where is each of the products produced in the overall process? Be specific. C 6 H 12 O 6 + 6 O 2 → 6 CO 2 + 6 H 2 O + Energy glycolysis oxidative phosphorylation Pyruvate to Acetyl CoA and Krebs cycle oxidative phosphorylation ATP/glucose, Glycolysis (2), Krebs (2 GTP), oxidative phosphorylation (up to 34) 2. In cellular respiration, the oxidation of glucose is carried out in a controlled series of reactions. At each step or reaction in the sequence, a small amount of the total energy is released. Some of this energy is lost as heat. The rest is converted to other forms that can be used by the cell to drive coupled energy-requiring reactions, or to make ATP. Fill in as much of the table below as you can before discussion. a. What is/are the overall function(s) of glycolysis and the conversion of pyruvate to acetyl-CoA? b. What is/are the overall function(s) of the Krebs cycle? c. What is/are the overall function(s) of oxidative phosphorylation? Oxidation of glucose to 2 pyruvate, generates 2 ATP and 2 NADH per glucose Oxidation of pyruvate/acetyl CoA to carbon dioxide. Generates 2 GTP, 6 NADH, and 2 FADH2 per glucose Oxidation of NADH and FADH2 to H2O (and NAD or FAD). generates H+ ion concentration gradient, therefore ATP. 3. For the table below, fill in where the compounds are used or produced. If neither used nor produced, leave the space blank. Are the compounds listed below used or produced in the processes listed at right? If produced, note the number of each produced per glucose molecule. Glycolysis The conversion of pyruvate to acetyl-CoA Krebs cycle Oxidative phosphorylation Glucose used O 2 used CO 2 produced (2) produced H 2 O produced
ATP Produced (4 generated-2used =2net) produced (2) produced (28) ADP + P i produced and used ? used (GTP) used NADH or FADH 2 Produced (2) produced (2) produced (6, 2) used NAD + or FAD used used ? used produced 4. Explain the difference between substrate-level phosphorylation and oxidative phosphorylation. How many ATPs (net) are made by substrate-level phosphorylation vs. oxidative phosphorylation in aerobic respiration? What role does chemiosmosis play? Substrate-level phosphorylation is a direct phosphorylation of ADP with a phosphate group using the energy obtained from a coupled reaction. Oxidative phosphorylation is the production of ATP from the oxidized NADH and FADH2. ( Substrate level phosphorylation - a phosphate group is transferred from a substrate to ADP. Oxidative phosphorylation - uses the energy released from the electron transport chain to generate ATP.) 5. The cell’s supplies of ADP, P i , and NAD + are finite (limited). What happens to cellular respiration when all of the cell’s NAD + has been converted to NADH? The cell can’t perform any processes that convert NAD+ to NADH, so glycolysis and the Krebs cycle would shut down since they both produce NADH.
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