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313 all 56 €7 BI 218 Food Microbiology S. aureus & E. faecalis Worksheet INSTRUCTIONS This worksheet is to be completed completely individually. Use your pre-lab work (as indicated in the lab manual), as well as your results from lab to guide you in making decisions about the mock data presented in some of the tables below. Staphylococcus aureus Part 1- Isolating S. aureus from your pudding sample Table 1: Fill out the following table using the DIFCO manual to determine what you would expect to see on MSA, BP, and VJ (selective & differential medias) for S. aureus Initial colour | Selective Ingredient Differential Ingredient & Expected colour reactions Indicators Mannitol Salt Agar Baird Parker + Egg Yolk Vogel and Johnson + Potassium Tellurite Table 2: After performing the experiment, you observed the following results for S. aureus. Indicate whether these results are what you expected, and report the final reportable counts for each medi Agar Counts & Final Answer Colony colour, agar colour, acid Was this expected? Explain. CFU Reportable Count fer ion, metallic sheen, etc. MSA Yellow colonies on yellow agar 42 BPA + Egg yolk Green metallic sheen colonies on yellow 30 agar . Mohammad Sharif AA & e.centennialcollege.ca ¢ < (M M