BIO 1090 midterm study notes

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University of Guelph *

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1090

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Biology

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Oct 30, 2023

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Outline Course email: BIOL*1090@uoguelph.ca Cell division: Morris: 11.1, 11.2, 11.5, 11.6 Spindle Assembly Checkpoint - Makes sure all chromosomes are attached to the spindle DNA damage checkpoint - Checks for damaged DNA before the cell enters S phase - Activates protein kinases that phosphorylate p53 - Turns on genes that inhibit cell cycle - This gives time to repair the damaged DNA or kill the cell DNA replication checkpoint - Checks for the presence of unreplicated DNA at the end of G2 before the cell enters meiosis Protein p53- found in the nucleus Cancers - Oncogene- Cancer causing gene - Proto-oncogene- normal genes important for promoting cell division that have the potential to become cancerous if mutated - Tumor suppressors- genes that encode proteins whose normal activities inhibit cell division Cancer steps 1. Normals cells 2. Benign cancer 3. Malignant cancer 4. Metastatic cancer
Cyclin-CDK complexes - G1/8 cyclin-CDK complex- Prepares cell for DNA replication - S cyclin-CDK complex- Initiates DNA synthesis - M cyclin-CDK complex- Prepares cell for mitosis Mitosis vs Meiosis - Mitosis - Ocecurs in the somatic cells - Results in two diploid daughter cells - Each daughter cell is genetically identical - Can continue for many cycles - Meiosis - Ocecurs in the germ (sex) cells - Results in four haploid daughter cells - Each daughter cell contains half the # of chromosomes as the parent - Each cell is genetically unique - Is aterminal event
Meiosis Prophase 1 1. Chromosomes become visible as thin threads. DNA replication is already complete 2. Homologous chromosomes continue to condense and undergo Synapsis(gene-for-gene pairing) 3. When synapsis is complete, each pair of chromosomes forms a Bivalent. Each chromosome has two sister chromatids 4. The chromosome shorten and thicken and the chiasmata between the non-sister chromatids become apparent 5. The nuclear envelope breaks down Prometaphase 1 2. Spindles attach to kinetochores on chromosomes Metaphase 1 3. Homologous pairs line up in the center of cell, with random bivalents oriented randomly with respect to each other Anaphase 1 4. Homologous chromosomes separate, but sister chromatids do not separate Telophase 1 and cytokinesis 5. Daughter cells are ready to move into prophase 2 (Each daughter cell has 1 set of chromosomes, so ploidy level is ‘n’ after cytokinesis of Meiosis 1) Meiosis 2 Prophase 2 1. The nuclear envelope breaks down and the chromosomes condense Prometaphase 2 2. Spindles attach to the kinetochores on the chromosomes Metaphase 2 3. Chromosomes align in the center of the cell Anaphase 2 4. Sister chromatids separate Telophase 2 and Cytokinesis 5. The nuclear envelope re-forms and the cytoplasm divides
Cell Division - Process cells make more cells - Ocecurs to allow growth, replacement, healing and reproduction - Occurs in the nucleus via : 1. Mitosis (Somatic or body cells) 2. Meiosis (Germ or sex cells) Eukaryotes - Genome large and linear - DNAn the nucleus Prokaryotes - Genome small and circular - DNAin the cytoplasm Main stages of cell cycle G1 Phanes (Gap 1)- Growth, cellular metabolism S phases (Synthesis- DNA replication G2 phase- Preparation for mitosis M phase (Mitosis)- Chromosomal separation and cytokinesis Interphase- The time between most successive mitoses (G1 +S) The human Karyotype Homologous chromosomes (autosomes) Prior to cell division the dna must be duplicated so that each daughter cell receives a complete set of chromosomes When counting the number of cells you don't count the arms, you count the centromere Ploidy - The number of sets of chromosomes in a cell is a ploidy (2N) - Acell with one set is an haploid (N)
Prophase - Chromosomes condense and become visible - Centrosomes concede and radiate microtubules and migrate to opposite ends of a cell Prometaphase - Microtubules of the mitotic spindle attach to the chromosomes - Nuclear envelope begin to break down Kinetochores attach to the mitotic spindle (Microtubules) on either side of the centromere. Metaphase - Chromosomes align in the center of the cell Anaphase - Sister chromatids separate and travel to opposite poles - They become individual chromosomes when the centromere splints Telophase - Nuclear envelope re-forms and chromosomes decondense Animal Cytokinesis Regulation of the cell cycle 1. Cell division cannot occur all the time because it can be dangerous and cause cancers
Mendelian Principles of Inheritance Gregor Mendel Discovered the Monohybrid Cross - Mendel followed the inheritance of a single trait - Each trait came in one of two forms: Dominant and recessive - Dominant and recessive forms of a gene are called alleles Alleles Uppercase- Dominant allele Lower case- Recessive allele Gene - Aninherited factor that determines a characteristic Allele - One or two or more alternative forms of a gene Locus - Specific location on a chromosome occupied by an individual organism Genotype - Set of alleles possessed by an individual organism Heterozygote - Anindividual organism possessing two different alleles at a locus Homozygote - Anindividual organism possessing two of the same alleles at a locus Phenotype - The appearance or manifestation of a characteristic Characteristic - An attribute or feature possessed by an organism Pedigrees - Autosomal Dominant -Appears in females and males equally -Unaffected people do not transmit the trait -Affected people have at least one affected parent -Is seen in every generation -When one parent is affected and the other isn’t, approx. half the offspring will be affected -Unaffected parents to not transmit the trait
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