E-Coli at Home Video Questions (1)

1- Why was the T4 and E.coli mixed together in top agar and then poured on the plate instead of just spreading the two together with a glass spreader on the plate? 2- When doing the titration and isolation of the T4 phage, E.coli diluted for you to 10e7 cells/ml. why was a diluted culture used? 3- After doing the titration of T4 phage, you find your dilution plates are all TNTC. how could this have happened? 4- Could you isolate phages that infect E.coli from raw sewerage? explain.

A microbiologist sets up a serial dilution to analyze bacteria in raw milk.   Tube 1 – 0.1 mL milk diluted in 9.9 mL sterile water Tube 2 – 0.1 mL milk from Tube 1 diluted in 9.9 mL sterile water Tube 3 – 1.0 mL milk from Tube 2 diluted in 9.0 mL sterile water   What is the dilution of raw milk in Tube 3?

I AM TRYING TO IDENTIFY THIS UNKNOWN. IMAGE 1 HAS TWO PICTURE OF CATALASE TEST AND BLOOD AGAR TEST. I believe it is one of the following: 1) S. pyo.    2)S. agal .     3)S.pneu.   4)E. faecalis                  5)S. aureus       6)S epi.       7)S. sapro.        8)M. luteus   Please let me know which test will i need out of the table to justify your reason of picking up the unknown and also how that test justifies it? what characteristics of that test made you pick the unknown?

true or false

additional 5 courses of cross contamination how to prevent cross contamination via kitchen utensils to foods how to prevent cross contamination via food to food

1. What kinds of media would be used to culture and Identify this microbe?2. What are some other potential microbes that could have this infection?

Please fill in the gaps .

1. An undercooked boiled egg was received in the microbiology laboratory with a request culture and identification.  This egg was served in a pack lunch during a birthday party.  All guests who ate this egg were brought to the hospital for vomiting and dehydration. Some had bouts of diarrhea.  The signs and symptoms were short-lived after treatment was received. The egg was homogenized in tryptic soy broth.  This was plated in sheep’s blood agar plate and MacConkey agar plate.  After 24 hours of incubation, the following observations were noted: Sheep’s blood agar plate:   4+ (many) beta hemolysis,  creamy white colonies MacConkey agar plate:    no growth The gram stain result of the isolate yielded gram-positive cocci, mostly in clusters.   If Staphylococcus aureus is suspected, list 3 tests that will be most relevant in its identification – i.e. to differentiate it from other gram-positive cocci

which agent was most effective against the fecal coliform that was tested? how does this agent kill bacterial cells?

1. Give 2 systems used at present for the automatic identification of bacteria. 2. Why aseptic technique important in urine collection for culture? 3. What are the specimen consideration you have to remember in preparing blood culture? 4. Being a medical technology student, how can you contribute to avoid water pollution and degradation of natural resources?

1. What is the most common source of E. coli? (i.e. where does it occur naturally) 2. Why do children seem to be the most ill? (Give at least 2 reasons) 3. What is the morphology, morphological arrangement and Gram reaction of E. coli? 4. How does the E. coli strain 026:H11 damage its victims?

True or false ?

Which of the culture given use of antibiotics amapicillin,chloramphenicol and steptomycin is most effective and least effective in a E. coli

What is the purpose of fixing a smear? Mark all that apply: 1. To attach the bacteria to the slide  2. To cause the cells to shrink and become distorted 3. To kill the bacteria so they aren't harmed by the staining method 4. To break down the cell wall in order to make the cells accept stain 5. To kill the bacteria to make the slide safer to handle

nt ● ● S ● Paragraph F 2. To determine if the type of agar affects bacterial growth, a scientist cultures on fou different types of agar. Five petri dishes are set up to collect results: One with nutrient agar and E. coli One with mannitol-salt agar and E. coli One with MacConkey agar and E. coli One with LB agar and E. coli Styles One with nutrient agar but NO E. coli Ed

Table C. Xanthoproteic Reaction Samples |1. Tryptophan 2. Tyrosine 3. Casein 4. Albumin 5. Peptone 6. distilled water Observation

I got it wrong can you help

Based on the pictures provided, tell me what I have for the gram-positive, and what I have for the gram-negative? Possible Unknown Strains: Staphylococcus epidermidis, Bacillus subtilis, Staphylococcus aureus, Bacillus megaterium, Eschericia coli, Pseudomonas aeruginosa Name one negative and one positive of which I have.

Based on the pictures provided, tell me what I have for the gram-positive, and what I have for the gram-negative? Possible Unknown Strains: Staphylococcus epidermidis, Bacillus subtilis, Staphylococcus aureus, Bacillus megaterium, Eschericia coli, Pseudomonas aeruginosa Name one negative and one positive of which I have.

Which cultures would be suitable for the transduction experiment and why？ Which cultures would not be suitable for the transduction experiment and why？

I need the answer as soon as possible

bacteriophage lab report:

MALDI-TOF, is a method for identifying bacteria quickly. Look at this graphic then put the steps in order. Please follow a pathway of steps involving the Bacterial Culture:                                   Get a readout of species identification and (sometimes) whether it is resistant or susceptible to antibiotics.                                 Use  colony from a nutrient agar plate.                                              Prepare the sample, run it through MALDI-TOF machine.                              Grow a clinical sample on nutrient agar.

A certain culture of the bacteria stripped her classes and national has 10 bacteria to double every 1.5 hours estimate the number of bacteria after 35 hours after how many hours for the bacteria count reach 10,000?

What type of media are culture medium 1 and culture medium 2? And its difference to each other?

This is how a hanging-drop slide is prepared. Figure from Macedo, Wikimedia Commons, 2016. 2 3 4 slide cover slip vaseline concavity drop of microbiological culture CA inoculation loop 11. Which slide gives you more information, the hanging drop or the stained slide? Why do you say so? 12. Why might it be valuable to know whether a bacterium is motile?

What are a couple of test that I should do for unknown bacteria microbiology identification lab? • My bacteria is gram positive • It is one of these 5 bacteria’s Enterococcus Faecalis Micrococcud Luteus Staphylococcus Aureus Staphylococcus Epidermidis Streptococcus Pyogenes

Why is Muller Hinton Agar (MHA) ideal for antibiotic susceptibility testing

19. What is the importance of group B Streptococcus agalactiae? 20. Starting with the MacConkey agar and IMVIC tests. Describe how a bacterium can be biochemically identified as Escherichia coli.