BIO 211 Blood Typing Questions

Plasma and clot e) Serum and clot 14. If small clots are noted when making a blood smear, one should: a) Make the smear and note on the slide that clots are present Mix the blood specimen for one hour then make the smear Do nothing; small clots will not affect a blood smear Request a new specimen before doing the blood smear Add more anticoagulant to the blood tube, mix, then make the blood smear 15. What is the purpose of doing a differential? a) b) To determine the proportion of RBCS in the whole blood To count the number of WBC's in whole blood To determine the proportion of WBC's in whole blood To microscopically examine RBCS and platelets To diagnose anaemia

BIO152L Worksheet for Howard Hughes Medical Institute: The Virtual Immunology Lab Name: Student ID number: Questions from ELISA Simulation Introduction Document (posted in Lab Module 8 folder): 1. What term is the word “antigen” derived from? 2. Where are antibodies found in the body? 3. In general, what is the cause of an autoimmune disease? 4. What kind of antibodies are present in patients with systemic lupus erythematosus (SLE)? 5. What does ELISA stand for? 6. In general, what is an ELISA test used to detect? 7. In an indirect ELISA, which antibody is linked to the enzyme? (i.e the primary antibody from the blood serum or the secondary anti-human antibody) Questions from HHMI Virtual Immunology Lab Website Diagnosis (The answers to the following question can be found in the “Diagnosis” tab) 8. What are the 3 important limitations of an ELISA? Explain each.…

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Patient # anti Rh 1 2 + 3 + 4 5 6 + + + Using information from the table above, match the blood type for each patient: Patient 1 Patient 3 Patient 4 Patient 6 Patient 5 Patient 2 anti A + + anti B + + AB+ O- B+ A- AB- B- A+ O+ I

PRE-LAB ACTIVITY (Adapted from "ABO blood typing with synthetic blood." Carolina Biological (2017). 1. Given the antigens presented below, determine the corresponding blood type and antibody(ies) that would be present in the plasma. Blood Type Red Blood Cell Antigen A* A- B B AB AB O' Antibody(ies) in Blood Plasma

MODIFIED TRUE OR FALSE. In the following items, read each statement carefully. I. About 20 L of fluids pass from the blood capillaries into interstitial spaces, whereas half of it pass from the interstitial spaces back into the blood capillaries.II. The function of the lymphatic system is to bring back the lost fluids back to the bloodstream to maintain fluid balance in our body.     a. The first statement (I) is correct and the second statement (II) is wrong b. The first statement (I) is wrong and the second statement (II) is correct c. Both statements are correct d. Both statements are wrong       MODIFIED TRUE OR FALSE. In the following items, read each statement carefully. I. Environment refers to the state of being and most importantly to the source and essentials of life. That is anything that exists in the world.II. Nature are all the conditions, circumstances and influences surrounding and affecting the development of an organism. That is anything that…

rom che of e Name:. Year and Section: EXERCISE NO. 24 Demonstration of Blood Cells Date Submitted: Group No.:. POST-DISCUSSION QUESTIONS I. Answer the following prompts: 1. Record the result of the differential count, and compare with the reference range. 2. A 45-year-old male was seen in the emergency room with complaints of fever and chills. Blood test reveals a WBC count of 15.5 X 109/L with a differential count of Neutrophil -78%, Lymphocyte - 20%, Monocyte- 02%. Urine analysis shows the presence of pus cells and positive protein. Given the laboratory result, what is the probable diagnosis?

INTRODUCTION LABORATORY SIMULATION Labels Granulocytes Lymphocyte Neutrophil Agranulocytes Basophil Monocyte Eosinophil Photo credit McGraw-Hil Education/Al Telser

A junior medtech working on a night shift. You were assigned to the blood banking section of the laboratory where you are designated to test blood for crossmatching and release blood bags. You release O positive blood bag for a patient with anemia. After 10 minutes, the nurse called and said that the patient is having a "reaction" to the said blood and told you to investigate and re-checked. What are the relative effects if the problem is unsolved in relation to the: medtech, laboratory, hospital, and patient?

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topic: blood (please do not copy from google)  2. describe the shape of the red blood cells.

Question. If you are working in a diagnostic lab, what will be your choice among Monoclonal or Polyclonal Antibodies method for detection of specific pathogen? Give sufficient justification for selection as well as rejection of any given methods.

Antibody Panel: Lewis Kell Duffy Kidd RH MNS LU PEG DCE + + f Lu Lu P: Le Le K k Ex Ex Jk: Jk IS 37 AHG e + NT Ov + + + + 2 + + + + + NT 3+ 3 + + + + + NT 2+ 4 + + + + + + NT 5 + + + + NT 2+ + + + + + NT 0 7 + + + + + + + + + NT 1+ 8. + + + + + + + + NT 2+ 9. + NT OV + + + + + + 10 + + + + + + NT 2+ NT Ov NT 0V 11 00 + + + + + + AÇ 2. Given the results of the antibody screen and antibody panel, which of the following antibodies CANNOT be ruled out? More than one answer may apply. а. Anti-Jk b. Anti-K c. Anti-Fy d. Anti-C 3. Which of the following antibodies would need to be ruled out with selected cells? More than one answer may apply. a. Anti-E b. Anti-Lu C. Anti-S d. Anti-D alele ++0 + +lo +c +Holc olola ulolol

Compute for the absolute count of patients A and B PATIENT A WBC count = 6 x 109/L Differential count Absolute count Neutrophils = 65%   Lymphocytes = 20%   Monocytes = 13%   Eosinophils = 2%     PATIENT B WBC count = 18 x 109/L Differential count Absolute count Neutrophils = 65%   Lymphocytes = 20%   Monocytes = 10%   Eosinophils = 5%   Give an impression of their results. (Indicate whether the cells are increased or decreased using the proper terminologies. Indicate also whether the increased or decreased values are absolute or relative)

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1. What is an antiserum? 2. What are the potency requirements in an antiserum? ANSWERS TO QUESTIONS ON LABORATORY ASSAY NO.4 3. What kind of antigen will anti-A detect? Anti-B? 4. Enumerate the common causes of false positive and false negative result in ABO forward grouping? 5. Give the purpose of Blood typing. 6. Cite the biochemical components of the ABO blood group accordingly. 7. Complete the table below Blood type A B 0 AB (positive/pos) for agglutination (negative/neg) for no agglutination Anti-A Anti-B

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Page of 4 ZOOM + 4 6. Determine the following blood types for those four donors. Look at the reaction in each well after adding Anti-serum: Blood Type Anti- A serum Anti- B serum Ant- Rh serum Alfie Nadia Ralph Sebastian

Composition of Blood 1. Determine the physical characteristics of plasma after it centrifuge for 5 minutes? 2. Differentiate the plasma from serum after letting it stand for 15 minutes and then putting it into centrifuge for 5 minutes 3. Microscopic Evaluation of Formed Elements of Blood - Describe the appearance of the erythrocytes, leukocytes and their different types, and the platelets - Discuss the functions of each of the formed elements in the blood and give their relative percentages.

# of inversions needed: Match the additives in the rows to the number of inversions needed after collection of sample

Asap I need (3).  When drawing blood from a patient for labs, what do the different tube/vial top colors repersent?

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Show all working explaining detailly each step Answer should be typewritten using a computer keyboard!

ursework 2 Coursework 2 Paraphrasing Tool - QuillBot Al Help I Shania Ghosh (21029789) I Close Remaining Time: 05:37:46 A researcher is following an immunohistochemistry protocol. Before blocking the tissue section, the researcher incubates the slide in citrate buffer at 100°C for 10 minutes. a) What is the name of this step? - b) Explain why this step is sometimes required before blocking and incubation with the primary antibody. I ΞΕΙΩΣΕgHglion A- A- T BIUS X, x Styles Font Words: 0/30 4

VIII. Blood Types Fill in the blanks to complete each of the following sentences. Choose from the words listed in the Word Bank. AGGLUTINATION HEMOLYSIS POSITIVE ANTIBODIES NEGATIVE RED BLOOD CELLS ANTIGEN PLASMA A AB ,of which there are two types (A 1. Each red blood cell carries a protein called an and B). 2. Plasma carries 3. A transfusion reaction occurs when the antibodies of one blood type attack the cells of the other blood type, causing the cells to clump together; the process of producing large clumps of cells is called 4. During a reaction, red blood cells may burst; this is called 5. Blood type completely accurate. Reactions usually do not occur because only the transfused. against the of the other blood types. is sometimes called the universal donor, although the term is not are being is sometimes called the universal recipient, although this is not 6. Blood type completely accurate either. 7. Many people also carry another antigen, called the Rh antigen; when this is the…

Patient Testing Solution (Mr. Smith) Reaction Phenotype (Blood Type) Possible Genotypes for blood type (Clumping? Antibody-A Antibody-B | Antibody-Rh clumping no clumping clumping A+ AA or AO 1. a) Using a Punnett square determines one possibility of what the patient's (Mr. Smith) parent's blood types could be. For the Punnett square, ignore the Rh+ and Rh-. b) Could the parents be a possible donor? Explain why or why not? You may consider Rh+ and Rh- here. c)Which donor would you recommend, if any? Explain your choice using concepts and vocabulary from the course. You may use words, drawings or diagrams to clarify your choice. (Approximately 1 paragraph in length). 20 tv MacBook Pro & %23 $ % 2 3 4 7 E R Y P D F K C V 36 MOSISO comm nd

Question. Correct the following statement. If a person RBCs have B surface antigen and it will clump with antigen B such clumping indicates Blood type B. Correct:

For each blood type in the table below, fill in the expected agglutination result from mixing the blood with eachof the antibodies.