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Week 2: Lab Document
Chamberlain University
March 13, 2023
OL Lab 2: Intestinal Glucose Transport: Study a mouse intestine model to diagnose an
infant
Learning Objectives:
1
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Understand the epithelial model for how glucose is transported across the mammalian
small intestine
Perform a glucose assay method
Use an animal model to study the transport of materials across the intestine
Describe the effect of sodium-potassium ATPase blocker on glucose transport by the
small intestine
Explain the effect of manipulations of mucosal concentrations of glucose and sodium on
glucose intestinal transport
Interpret physiological data and apply to clinical cases
In this simulation, you will learn how glucose absorption occurs in the intestine.
You will help treat an infant who has trouble gaining weight. She is experiencing diarrhea, with
elevated blood sodium concentration and glucose in her feces and urine. Dr. Shaw, your
supervisor, suspects this could be related to the poor glucose absorption. You will use a piece of
mouse intestine as your model to study intestinal glucose transport and see how your findings
can be used to diagnose human diseases.
Study glucose transport by measuring its concentration:
After clearing out the impurities from the everted intestinal piece, you will expose both the inner
(mucosal) and outer (serosal) sides to glucose solutions of different concentrations, a sodium-
potassium ATPase blocker, and a sodium-free saline solution.
After these treatments, you will measure the glucose levels on both sides, and analyze your data
to understand the active glucose transport through the intestinal epithelium. Also, a step-by-step
interactive summary diagram will help you visualize what happens to all the molecules and
transporters involved.
Will your findings be able to help make the infant well again?
Part 1: Complete Labster- Intestinal Glucose Transport: Study a mouse intestine model to
diagnose an infant
2
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Part 2: Report and Reflection
Purpose: Describe in your own words and in complete sentences, the purpose of this experiment.
-The purpose of this experiment was to understand how glucose works and how it’s transported
into the intestine
Observations: List 2 observations you have made in this simulation.
1. The active transport of glucose is higher on the serosal side when glucose is being transported
from the mucosal to the serosal side
2. As the intestine is inflated with the catheter, it expands to better see the effects of glucose
absorption
Answer all the questions below:
1. How does sodium regulate the uptake of glucose into enterocytes? (3 points)
-It regulates the uptake of glucose into the enterocytes by using a sodium glucose transporter
which transports glucose across the cell membrane. It also uses a co-transporter which also helps
with glucose uptake.
2. In the simulation, why did the infant experience diarrhea due to glucose malabsorption?
(3 points)
-The infant experienced diarrhea because she’s unable to absorb glucose. The glucose remains in
the intestines which increases the amount of water present since that water is unable to cross the
brush border with the glucose resulting in diarrhea.
3. What causes lactose intolerance? What are the products of lactose breakdown? (2 points)
3
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Related Questions
Please help me solve questions 1,2.
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asap please.
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RON is suspected of DKA. Formulate a plan of education for Ron's wife, so she will better understand his condition and be able to assist him to manage those days when is unwell. What areas would you cover? How would you ascertain her understanding?
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2:26 PM O 10 O
39
GRADE10_LAS4 (1)...
LEARNING ACTIVITY SHEET
GRADE 10 - SCIENCE
Name:
Date:
Rating/Score:
ADAPTATION AND SURVIVAL
DIRECTIONS: This Learning Activity Sheet is about the adaptation and survival
organisms in a changing environment. Follow the instructions in each part and answ
the guide questions that follow.
PART A. My Bio-cabulary
Explain each of the given terminologies in your own words then answer the
question.
Terminologies
How will I Remember
Biodiversity
RICH
CY AS
Stability
Adaptation
NCR
Survivability
Think About It!
Based on the answers above, explain how species diversity increases the
probability of adaptation and survival of organisms.
Specific Week: 7 (LAS 4)
Target Competency: Explain how species diversity increases the probability of adaptation and survival of orga
changing environment (S10LT-IIlh-41)
(This is a Government Property
For Sale
LEARNING ACTIVITY SHEET
GRADE 10 - SCIENCE
PART B. Adaptation and Survival
SEXCEL
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BRIEF EXPLAINATION
5. What Is the function of insulin, glucagon and epinephrine?
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Please explain this steps by steps and make it easy way to understand
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NURSERY WARD SCENARIO
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ntegrated LabCase Study DiabetesJohn is a 50 yr old man admitted to your medical unit with an ulceration on his right ankle. His blood glucose level is 473. He tells you he takes NPH (Humilin N) insulin 40 units every morning and Regular insulin with each meal and at bedtime.John’s first dose of insulin each day is given at 6 am. Chart the action of this insulin throughout the day, showing what time the insulin was given and where it peaks.Hint: This is referring to the NPH. Look up peak times for this.John said his doctor told him to keep his glucose between 100 and 150. What is the normal range for blood glucose.
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Complete the crossword.
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pathophysiology
A client is on complete bed rest after a total hip replacement surgery while he is being treated for a mild postoperative surgical wound infection. Identify the underlying physiologic factors that could increase the nutritional needs of this client. give scientific rational
A.Anabolism, polydipsia, hypertriglyceridemia
B.Elevated protein plasma levels, hypotension, glycolysis
C.Large abdominal girth, insulin resistance, acidosis
Fever, increased white blood cell count, negative nitrogen plasma level
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Q13: what is the correct answer
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Q13
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Pls help ASAP.
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Pls help ASAP.
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Present in clear scanned handwritten complete solutions or in typewritten thank you
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Physiological Systems and Regulation
Assessment 2: scenario based assessment
Instructions
This is a ‘scenario problem’. Read the information and answer the questions. Your answers must be concise, focus on the question (an introduction isn’t needed), and include a short explanation. ANSWERS OF ESSAY LENGTH ARE NOT REQUIRED. You will need to include some explanation as part of your answers. References should be included both in text (Harvard referencing system) and as a reference list at the end. An exemplar based on last year’s scenario is available on Moodle to guide you on the level of detail expected for this assessment.
Please note that these questions are not equally weighted, and it is likely that answers to some will be a bit longer than others. Because of this, your mark will be based on your overall response to all of the questions. This doesn’t mean you can ignore a question – you must attempt all questions.
Consider the following scenario:
A group of five treasure…
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Give typed full explanation of all subparts
Lawrence has been taking his prescription for the last month and had his blood drawn. At his follow-up appointment with his physician, he complains about muscle cramping in his legs that is particularly bad at night and it is not responding to the non-pharmacological treatments he’s tried, including hot/cold packs, warm baths or muscle rubs. He states that he is worried that he won’t be able to keep taking the medication if the side effects will remain as long as he is taking it. 1. What adverse effect is Lawrence experiencing? Why is this important? Lawrence’s doctor instructs him to only take his statin on alternating days, and to add Omega-3 Fatty Acids 800 mg per day, to supplement his treatment. Lawrence is to have his blood drawn again in one month and schedule another follow-up appointment to review his lipid panel with his doctor. 1. What purpose does changing Lawrence’s dose to every other day serve? 2. What are Omega-3 fatty acids…
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How can pulse oximeter be used to diagnose hypoventilation? What is the limitation of the device from reading the article “Obesity Hypoventilation Syndrome”?
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Fluorescent in situ hybridization for 15q11 shows no microdeletion Methylation studies show a lack of paternal contribution at the 15q11 locus. Which of the following is the most likely genetic mechanism of these
findings?
A) Anticipation
B) Heteroplasmy
C) Imprinting
D) Non-paternity
E) Pleiotropy
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Please help me with these questions. Thank You!
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No Service e
12:23 PM
82%
PATHOLOGY Closed book
Remaining
00:37:45
* Q 2. A 55 year old male alcoholic presents
with symptoms of liver disease and is
found to have mildly elevated liver
enzymes. A liver biopsy examined with a
routine hematoxylin and eosin (H&E) stain
reveals abnormal clear spaces in the
cytoplasm of most of the hepatocytes.
Which of the following materials is most
likely forming these cytoplasmic spaces?
()
A. Calcium
B. Anthracotic
C. Hemosiderin
D. Lipofuscin
E. Triglyceride
* Q 3 Several days after exploring a cave in
eastern Kentucky, a 39-year-old woman
dovolons shortnoss of breath and a low-
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Please asap
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MAKE A SCIENTIFIC (must include author and year published) AND SITUATIONAL ANALYSIS OF THIS DX.
SCIENTIFIC ANALYSIS:
SITUATIONAL ANALYSIS:
Main Dx: Imbalanced nutrition less than body requirements related to Lacto-Ovo vegetarian diet as evidenced by lack of essential minerals & nutrients (Iron, Zinc & Omega Fatty-acids)
SCENARIO: Aubrey, 19 years old, an incoming college freshman student went to Ateneo De Manila Health Services for physical examination. The nurse gathered the following information: Height: 5 ft Weight: 48 kg Vital signs: Temp 37.2C, Pulse rate: 95 beats per minute, Respiration rate: 12 breaths per minute, and BP: 100/70mmHg. Family History of illness: Father (+) Hypertension (HPN), and Diabetes Mellitus (DM) Present Health History: Aubrey never experienced to get hospitalized as far as she can remember. Her mother ensures that she takes daily supplemental vitamins such as vitamin C and B complex, and every year she gets flu vaccine from their family doctor.…
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Please I need help understanding this question
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Hint: Answers may be chosen more than once.
Haematoxylin & Eosin. option 1-6 is between the spleen, adrenal gland, pancreas, lymphoid tissue, salivary glands, thyroid glands, ears.
option 7-10 is between the oesophagus, colon, small bowel, liver, tongue, spleen
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SEE MORE QUESTIONS
Recommended textbooks for you
Anatomy & Physiology
Biology
ISBN:9781938168130
Author:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark Womble
Publisher:OpenStax College
Related Questions
- Please help me solve questions 1,2.arrow_forwardasap please.arrow_forwardRON is suspected of DKA. Formulate a plan of education for Ron's wife, so she will better understand his condition and be able to assist him to manage those days when is unwell. What areas would you cover? How would you ascertain her understanding?arrow_forward
- 2:26 PM O 10 O 39 GRADE10_LAS4 (1)... LEARNING ACTIVITY SHEET GRADE 10 - SCIENCE Name: Date: Rating/Score: ADAPTATION AND SURVIVAL DIRECTIONS: This Learning Activity Sheet is about the adaptation and survival organisms in a changing environment. Follow the instructions in each part and answ the guide questions that follow. PART A. My Bio-cabulary Explain each of the given terminologies in your own words then answer the question. Terminologies How will I Remember Biodiversity RICH CY AS Stability Adaptation NCR Survivability Think About It! Based on the answers above, explain how species diversity increases the probability of adaptation and survival of organisms. Specific Week: 7 (LAS 4) Target Competency: Explain how species diversity increases the probability of adaptation and survival of orga changing environment (S10LT-IIlh-41) (This is a Government Property For Sale LEARNING ACTIVITY SHEET GRADE 10 - SCIENCE PART B. Adaptation and Survival SEXCELarrow_forwardBRIEF EXPLAINATION 5. What Is the function of insulin, glucagon and epinephrine?arrow_forwardPlease explain this steps by steps and make it easy way to understandarrow_forward
- NURSERY WARD SCENARIOarrow_forwardntegrated LabCase Study DiabetesJohn is a 50 yr old man admitted to your medical unit with an ulceration on his right ankle. His blood glucose level is 473. He tells you he takes NPH (Humilin N) insulin 40 units every morning and Regular insulin with each meal and at bedtime.John’s first dose of insulin each day is given at 6 am. Chart the action of this insulin throughout the day, showing what time the insulin was given and where it peaks.Hint: This is referring to the NPH. Look up peak times for this.John said his doctor told him to keep his glucose between 100 and 150. What is the normal range for blood glucose.arrow_forwardComplete the crossword.arrow_forward
- pathophysiology A client is on complete bed rest after a total hip replacement surgery while he is being treated for a mild postoperative surgical wound infection. Identify the underlying physiologic factors that could increase the nutritional needs of this client. give scientific rational A.Anabolism, polydipsia, hypertriglyceridemia B.Elevated protein plasma levels, hypotension, glycolysis C.Large abdominal girth, insulin resistance, acidosis Fever, increased white blood cell count, negative nitrogen plasma levelarrow_forwardQ13: what is the correct answerarrow_forwardQ13arrow_forward
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SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- Anatomy & PhysiologyBiologyISBN:9781938168130Author:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark WomblePublisher:OpenStax College
Anatomy & Physiology
Biology
ISBN:9781938168130
Author:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark Womble
Publisher:OpenStax College