Dave Biochemical Tests LAB REPORT (1)

Effect of pH on Microbial Growth 12 10 8 Escherichia coli -Pseudomonas aureus -Staphylococcus aureus -Listeria monocytogenes 1 4 pH Minimum Optimum Maximum Escherichia coli 4.5 7 Pseudomonas aureus 4.5 7.5 9.5 Staphylococcus aureus 6.8 Listeria monocytogenes 4.5 7 9.6 Make a discussion on the experiment of the effect of pH on microbial growth. Growth Rate of Microbial 2.

Sample Station 1 This is an Eosin Methylene Blue (EMB) streaked with a pure culture. Looking at the growth and color in the image, answer the following: A. What is the expected Gram reaction and morphology of this organism? B. What does the color of the growth tell you about the organism's ability to ferment lactose to acids?

Give only typing answer with explanation and conclusion

Phenol Red Test Question: Assuming you tested an organism using Phenol Red glucose and Phenol Red Base broths, which of the combinations of results in the following table would be reliable. Interpret each of the combinations and explain how the results might be obtained

71-80. Draw the following structure with their coresponding functional groups inside the box with at least 4 carbon stoms. 71. Ether 72. Anhydride 73. Primary alcohol 74. Secondary akohol 75. Aldehyde 76. Ketone 77. Amine 78. Amide so. Carboxylik Acid 19. Ester

Catalase test What is the reagent used in this test?

The first choice : ori lacZ blaz araC GFP The second choice : arabinose Ampicillin  X-gal IPTG GFP The third choice : X-gal lactose arabinose GFP Ampicillin The fourth choice : on ice at 42℃ at 95℃ at 37℃ at RT The fifth choice : arabinose ampicillin lactose X-gal GFP

Sulfur Indole Motility (SIM) Medium H2S produced, color and +/-: ______________________________ Indole present/Tryptophan hydrolysis, color and +/-: ___________________________ Motile or non-motile: _____________________________

Catalase Yeast Test Questions: 1. Why is it important to graph volumes that did not include the initial volume? 2.  How might the temperature of the yeast mixture affect a reaction? 3. What effect would increasing the amount of yeast have on a reaction?

Phenol Red Test Question: what would be the outcome of innoculating fresh cultures of Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecalis inside the phenol red broth after incubation?

Citrate test What is the only souce of carbon in this medium?

Original culture 50 colonies from .1mL, 1:10000  estimate the number of bacteria per unit volume in the original culture.

true or false

1 %Ar l. docs.google.com/forms/d/e O 1. False Endospores can be inactivated by high pressure and high temperature. O True O False The bacterial capsule is known to be highly resistant to various forms of radiation and other physical and chemical agents. * O True O False Aldehyde has a broad spectrum of activity against bacteria, fungi and viruses, and can العربية الإنجليزية

Description 1. The fractions obtained from differential centrifugation are enriched but not pure. Explain how a greater degree of purification can be achieved using Density-gradient centrifugation and velocity centrifugation. 2. Explain equilibrium centrifugation.

describe the liquid growth analysis in detail.

VII - BIOMOLECULES Instructions : - Answer the question properly. - Please do not copy here in Bartleby - Explain why did you choose that answer in 3-5 sentences.

Topic: Isolation of Crude Ovalbumin from Egg White by Ammonium Sulfate Precipitation(Salting Out) The computed amount of powdered ammonium sulfate was added to the egg white sample portion byportion with constant stirring while submerged in an ice bath. The solution is expected to become moreturbid, and a white precipitate is expected to form.   Explain the purpose of using powdered ammonium sulfate in the isolation of ovalbumin from egg whites.

Table D. Millon's Reaction Observation Samples 1. Tyrosine 2. Casein 3. Albumin 4. Gelatin 5. Peptone 6. distilled water

DQuestion 21 GC and HPLC were used in this course as: O synthetic techniques analytic techniques purification techniques O liquid-liquid extraction techniques D Question 22 Based on the boiling point of acetone (56° C), 3-methylbutylacetate (141°C), and 3-methyl-1- butanol (131°C), which compound will be the first to come off (elute) the GC column? O acetone O 3-methylbutylacetate O 3-methyl-1-butanol

DIFFERENTIAL TESTS/CHO METABOLISM 6. You streak out E. coli and B. subtilis each onto a starch plate. You incubate the plates overnight, and the following day you flood the plate with iodine and you obtain the results shown below: Е. coli B. subtilis Describe what you see on the plates. What can you tell about B. subtilis and E. coli based on these results? (4)

Qualitative Analysis of Proteins 1. Fill out the table below by providing the necessary information indicated per column.  2. As you have virtually conducted these qualitative tests for proteins, what do you think could be the essential practical or real-life applications of these tests?

Do not copy paste from other websites

topic: Isolation of Crude Ovalbumin from Egg White by Ammonium Sulfate Precipitation (Salting Out) Aside from ovalbumin, what are other possible proteins which can be isolated from egg white? Give their biological significance.

Lab Data - X Phase 1 Plate Dilution Label Growth Appearance on Plate Isolated Colonies Present? Countable? # 1 1:10,000 Нeavy No Too numerous to count 1:100,000 Нeavy Yes, few Too numerous to count 3 1:1,000,000 Intermediate Yes, many Countable 4 1:10,000,000 Scant Yes, few Too few to count 000,00001:1 Image Plate 1 Plate 2 Plate 3 Plate 4 Phase 2 Plate Selected For Counting Plate 3 Volume of Diluted Sample (mL) 1 Sample Dilution 1:1,000,000 Colony Count 53

Advantages and disadvantages of the following dosage forms: ○ Tablets○ Capsules○ Syrups○ Suppositories○ Emulsions

Decarboxylase test (5-8). Why is the production of an anaerobic environment in an inoculated decarboxylase broth necessary to test for the bacteria's ability to produce the decarboxylase enzyme?

Citrate test What critical enzyme allows the organism to acquire carbon from the  medium?

COLOR REACTION OF PROTEINS Question Guide: 1. Write the positive result, the chemical group responsible for the positive and the importance of each test. Test Positive Result Chemical Group Biuret Ninhydrin Xanthoprotei Millon's Hopkins-Cole Lead Acetate Pauly Bromine Water Sakaguchi

Tables A & B. Ninhydrin and Biuret Reactions Samples TESTS Ninhydrin Biuret 1. Glycine 2. Casein 3. Albumin 4. Gelatin 5. Peptone 6. distilled water

URGENT Conjugate base questions

ISOLATION AND PURIFICATION OF PROTEINS

Why do cells need to be kept in the freezing devices first then transferred to liquid nitrogen storage in crypto preservation? In addition, why are antibiotics not used in the cell freezing media? Why FBS concentration higher than 10% (10% is the standard concentration in complete growth media) is recommended in cell freezing media?

My question is: Could you explain steps? (etc. why do we heat or add chemicals like nacl or ammonium sulphate?) 1)Casein Isolation from MilkExperimental Procedure* Put 100 ml of milk in a 500 ml beaker and heat it to 40 0C. * Also heat 100 ml acetate buffer and slowly add it to the beaker with milk while stirring. * The final pH of the mixture should be 4.8. * Cool the suspension to room temperature and wait 5 more minutes before filtering. * Wash the precipitate several times with small volumes of water and then suspend it in approximately 30 ml of ethanol.Filter the suspension in the buchner funnel and wash the precipitate a second time with a mixture of equal proportions of ethanol and ether. * Finally, wash the precipitate on filter paper with 50 ml of ether and soak and dry. * Make the ether evaporate by spreading the powder. * Weigh the casein and calculate the percentage yield of the protein. * Compare with theoretical yield of 3.5 g per 100 ml milk. 2)Purification of Egg…

Purpose Solve the identity of an unknown bacterial specimen by creating a dichotomous key and using the staining, culturing and biochemical identification procedures you have learned about during the semester. Possible Organisms Alcaligenes faecalis Enterobacter aerogenes Enterococcus faecalis Escherichia coli Proteus vulgaris Pseudomonas aeruginosa Salmonella arizoniae Staphylococcus aureus Staphylococcus epidermidis Staphylococcus saprophyticus Streptococcusbovis Streptococcus pyogenes You must write up your OWN dichotomous key for all the possible unknown organisms listed on above. Writing this key requires you use the same type of reasoning used in the Dichotomous key lab. The first step of the key will be the Gram Stain. Subsequent steps will include biochemical tests only. Please help me with this question. Thank you so much !