BSC2086C_Template_LabReport (1)

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Florida State College at Jacksonville *

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2086C

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Biology

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Dec 6, 2023

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docx

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6

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BSC2086C: Anatomy & Physiology II Lab Report for Lab 6: Carbon Dioxide and Blood pH Student Name: Haley Ajeel Purpose The purpose of this lab is to see the effects that carbon dioxide concentration has on blood pH. Hypothesis If additional carbon dioxide deposits are present in water, the pH will rise. Experiment Materials : Willing subject (someone to complete the activities of the experiment) Timer (with a second hand) Drinking glass Urinary and Salivary pH (litmus) paper - do NOT use ordinary litmus paper. The paper must test pH intervals at 0.2 pH) Drinking straw Procedures : 1. Photograph each step to include in your report 2. Measure 2 ounces of water in a glass. Obtain the pH of the water in the glass. Photograph and record the water pH prior to the experiment. 3. Have the subject blow air through the straw into the water and keep blowing into the water for 2 minutes inhaling between exhalations. The subject should breathe as normally as possible while doing this activity. Photograph and record the pH of the water in the glass. 4. Empty the water from the glass and refill it with fresh water. Have the subject do vigorous exercise for 5 minutes. 5. Immediately after exercise have the subject blow into the water for 2 minutes (inhaling between exhalations). Photograph and record the pH of the water in the glass. 6. Empty the water from the glass and refill it with fresh water. Have the subject rest for 5 minutes. 7. Have the subject blow in the water for 2 minutes (inhaling between exhalations). Photograph and record the pH of the water in the glass. Analysis/Data Results Control Air bubbles (2 min) Exercise (5 min) w/ Air bubbles (2 min) Rest (5 min) w/ Air bubbles (2 min) pH: 5.8 pH: 6.0 pH: 6.2 pH: 5.5 Table 1
Figure 1
Figure 2
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Figure 3
Figure 4
Conclusion/Discussion The overall trend during the experiment was if we increase carbon dioxide concentration in blood, or in this case water, the pH level would rise. This falls in line with the hypothesis. However, increasing carbon dioxide concentration in water/blood would actually lower pH (increasing acidity). I believe this experiment was performed incorrectly due to the litmus paper being extremely difficult to compare the coloration. Overall, the trend of pH never went above 6.2, meaning it stayed within acidic parameters. If the experiment was repeated, the vigorous exercise would need to be repeated with more intensity, and better lighting may assist in determining the correct coloration of the litmus paper. Chart 1
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