BIO 326 Winter 2023 Lab Exam Review (1)

Please help me understand this question

Answer these questions by watching the YouTube videos and reviewing the Powerpoints from Lab #4. 1. What is Refraction of light? 2. What is the difference between the Ocular lens and the Objective lens? 3. What is the purpose of the Revolving Nosepiece? 4. What is the difference between the Course Adjustment Knob and the Fine Adjustment Knob? 5. How do you calculate Total Magnification? 6. What is Resolution in terms of Microscopy? 7. What is the purpose of Oil Immersion? 8. What is the Diffraction Barrier and why does it exist? 9. What is the purpose of using stains and fluorescent dyes in microscopy? 10. What is the advantage of using an Electron Microscope? 11. What Objective lens should you always start with? 12. What is the purpose of the Iris Diaphragm on the Condenser? 13. How do you know your Objective lens has been adjusted properly? 14. Why should you not use Kimwipes…

Please see attached . Thank you

Please help make this procedure a flow chart

Please help me solve question 4 and activity 3 questions 1and 2.

Can you help me with this question?

Procedure 1 Magnification Light is refracted through two lenses to obtain magnification-the ocular lens and the objective lens. Magnifi- cation of the ocular lens is usually 10x.This means that if you were to view a slide with only the ocular lens, the image would be magnified 10 times. Magnification of the objective lenses varies, but typically is 4× for low power, 10× for medium power, and 40× for high power. (To verify that this is the case for your microscope, look at the side of the objective lens, which usually is labeled with its magnification.) Remember that oil immersion provides even greater magnification at 100×. When calculating the total magnification, you must multiply the magnification of the ocular lens (10) by the power of the objective lens. Fill in Table 3.1 to determine total magnification at each of the different objective lenses on your microscope. Remember that the magnification of the objective lens is usually printed on the side of the lens itself. ABLE 3.1…

Additional Practice: Parts of a Microscope Completing the table below is optional. However, you will be tested on these terms on Lab Exam #1 and are strongly encouraged to complete and understand this table. Microscope Part Eyepiece Objective Lenses Stage Diaphragm Light Source Arm Coarse Focus Adjustment Knob Fine Focus Adjustment Knob Function Description & Location on Microscope

Answer the following questions and uploa assignment. 1. What is the difference between a zoom knob and focus knob? 2. What is another name for the ocular lens? 3. Define working distance. 4. When would you use top illumination vs. bottom illumination? 5. What two parts of the microscope should you touch when carrying it? MacBook Air 吕0 F3 F4 F5 F6 F7

Depth of focus  1. Which colored thread comes into focus first, second and third (as observed under 10X)?    2. As observed under 40X and 4 mm objectives?

answer the following: instruction.match the name of the major part (listed below) with its location on the microscope, and give a very brief description of what each is used for:

please help me make this procedure a flow chart   III. Procedure: A. MicroscopyOperating Procedure:Place the microscope close to the edge of the table. Select a suitable stool so that when looking into the eyepiece, your back is straight and your neck is bent at the nape.1. Lower the body tube by turning the course focus knob until the 10x or 16mm objective reaches the downward stop.2. Look through the eyepiece and adjust the mirror to the position which provides the brightest and the most evenly illuminated field of vision which is the circular area seen in the eyepiece. Raise the condenser until its top lens at the same level as the stage. Place the slide on the stage and fasten it using the stage clips.3. Position the specimen area of the slide over the center of the stage aperture.4. Looking through the eyepiece, raise the coarse focus knob until the image appears. Focus as sharply as possible. Low power objective has a much greater depth of focus and is generally used for the…

Hi! Please include your reference. Thank you.

List down 5 steps in the given procedure below for the proper use of microscope that you think emphasized on proper equipment care and briefly explain why you think so in 1-2 sentences per identified step. 1. Connect the microscope to the power supply. Turn “ON” the microscope.2. Rotate the light intensity adjustment knob to adjust the brightness.3. Place the slide with the specimen facing upwards on top of the mechanical stage. a. Open the bow-shaped lever of the stage clip outward.b. Slide the specimen from the front toward the rear.c. Return the bow-shaped lever gently.d. Center the specimen over the aperture on the stage. 4. Use the Low Power Objective. a. Rotate the revolving nosepiece until the 10x objective lens is “clicked” into position.b. Rotate the condenser focus knob to bring the condenser down to the bottom and partially open the iris diaphragm.c. Rotate the coarse adjustment knob to focus the image. Move it as far as it will go without touching the slide.d. When coarse…

Can you provide short answers for these questions ?

RESULTS for Exercise 4: Microscopic Measurements Name Date Calibration of Microscope: PART A 1 Division on the Ocular Micrometer corresponds to: Objective Scanning Low Power μη High Dry um Oil Immersion um Show calculations for each objective: Exercise 4 Microscopic Measurements 35

Skill-Building Exercises (Question numbers refer to the numbered steps in the actual lab.) Finding the object 1. Letter "e" as it appears to unaided eye: 3. Letter "e" as it appears in microscope (total magnification 40 x)

Please see attached . Thank you

3 6 9 12 V Stage control knob 1. Mechanical support - is used for carrying the microscope Fine adjustment knob 2. Platform upon which specimen is placed Objective 3. Brings object into approximate focus Diaphragm/Iris 4. Eyepiece/ocular 5. Collects light from source and focuses it upon specimen Controls amount of illumination used to view the object; impacts contrast Stage nation Base 6. Lenses you look into 7. Provides more precise focusing on the object 8. Revolving device that holds the Condenser objective lenses 9. Provides additional magnification of Arm varying powers 10. Mechanical support rests on flat Nosepiece Coarse adjustment knob Stage clip surface of table 11. Hold slides in place 12. 122 Provides mechanical means to move the stage

State the function of each part of the compound light microscope.

Instruction: FILL IN THE BLANKS     1. For initial focusing of the specimen the ___________ (objective) is aligned with the body tube and the central opening in the __________.   2. To view the specimen on the stage, secure the slide with the __________. Make sure the slide is in line with the ____________ and the stage opening.   3. Adjust the ______________ to regulate the amount of light required. Adjust the _____________ properly so that optimum light is reflected in the microscope.   4. Manipulate the ________________ knob to bring the objective about 1 to 2 mm away from the slide. Never look into the ____________ when bringing the objective close to the slide to avoid damaging or crushing the slide. A clear field of vision should be ___________ not gray.   5. In viewing objects under the microscope, it is observed that the microscope ______________ the direction of movement and _______________ the position of the image observed.   6. In shifting to higher magnification, simply…

Instruction: FILL IN THE BLANKS.   1. In viewing objects under the microscope, it is observed that the microscope ______________ the direction of movement and _______________ the position of the image observed.   2. In shifting to higher magnification, simply rotate the _________________ to the desired objective. If the image is not clear manipulate the __________________ knob.   3. The total magnification of the specimen as seen in the microscope is 400x. This means that the ocular magnification is _______ x and the ______________ is 40x.

CALA Learning Task 2: Copy the chart below in your answer aheet. Identify the parts of the microscope listed inside the box. Group them according to their basic function. base body eye piece tube arm course adjustment mirror diaphragm fine adjustment knob objective lens nosepiece stage Magnifying luminating Mechanical PIVOT 4A CALABARZON Science G7

Anatomy and Physiology I Chapter Three The Microscope Exercise 3.5: Determining Depth of Field 6. Referring back to exercise 3.5, record the answers to the questions about colored threads in the spaces provided. 7 Color of bottom thread: Color of top thread: Color of middle thread: * Explalm why proper microscope technique requires always viewing a slide with the scanning objective first before moving to higher-power objectives. Use the concept of depth of field in the explanation. 7 Exercise 3.6: Parts of a Dissecting Microscope 8. Identify two differences between a dissecting microscope and a compound microscope. 8 a. b. 9. List three types of specimens a dissecting microscope might be used to view: a. b. Can You Apply What You've Learned? 10. What microscope structures are used to control the amount of light illuminating the specimen? 11. What happened to the light intensity when switching from low to high power? 12. What adjustment will typically have to be made to the light after…

please help me answer questions 4 & 5. thank you.

Please help with above

TOPIC: KATO-THICK & KATO-KATZ TECHNIQUES 1. What are the advantages and disadvantages of Kato-Katz technique? Briefly explain each.

Compute for the total magnification of the following: Ocular Objectives Total Magnification 5x Scanner 5x Low Power 10x Scanner 10x High Power 10x Oil Immersion 15х Scanner 15x Low Power 15x Oil Immersion

9:06 LTE 4) Answer true or false to each of the following statements. On high power, you should use the coarse adjustment knob. The diaphragm determines how much light shines on the specimen. The low power objective has a greater magnification than the scanning objective. The fine focus knob visibly moves the stage up and down. Images viewed in the microscope will appear upside down. If a slide is thick, only parts of the specimen may come into focus. The type of microscope you are using is a scanning microscope. For viewing, microscope slides should be placed on the objective. In order to switch from low to high power, you must rotate the revolving nosepiece. The total magnification of a microscope is determined by adding the ocular lens power to the objective lens power.

Can you help me to fill in the functions, please?

nsert Format Arrange View Share Window Help Biology Lab Exam 1 Sp 21 目 125% v Collaborate Insert Table Chart Тext Shape Media Comment View Zoom Add Page 3. (1 point) Show the answer to the following question in correct significant figures. 10.219 + 3.12 = 4. (7 pts) True or False. Circle the correct answer. True or False. A blank used to zero of spectrophotometer always contains just distilled water. True or False. The mode is the most commonly occurring number in a data set. True or False. One milliliter (mL) equals 1,000,000 microliters (µL). True or False. Peroxidase activity increases when the enzyme is boiled True or False. Enzymes are carbohydrates. True or False. Denaturing involves an enzyme breaking down into individual amino acids. True or False. Hydroxlyamine is a competitive inhibitor of peroxidase. 5. (1 point) How many µL (microliters) are in 2.32 L? Show your work for full credit. 6. (1 pts) Convert 1,745 mm tọ Km. Show your work for full credit. MAR étv 9. MacBook Air