BIO 2051 upcoming exam 2 practice test chs 7,9,12

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Lab 1.5 - Prelab Name: Lab Period: Electrophoresis of Serum Proteins Review Material 1. What does the prefix deci- mean? 2. How many moles of H* ions are released by 3 moles of HCl moelcules? 3. Where is adipose tissue found in the body? 4. What is the function of transitional epithelium? 5. Where is hyaline cartilage found in the body? 6. What is the function of bone (osseous tissue)? 7. How many chromosomes will be found in a human skin cell? 8. How many genomes are in a cell at metaphase I? 9. During which phase of mitosis does the nuclear envelope re-appear? 10. During which phase of meiosis does crossing over occur? New Material 1. Define electrophoresis. 2. Draw the general structure of an amino acid. 3. What is an amphoteric molecule? Give one example. 4. Why is it necessary to use a solution of pH 8.0 or higher during this experiment? 5. Distinguish between the terms homogeneous and heterogeneous. 6. Have you watched and attempted to understand the following YouTube video about…

TASK 1. From a 100-mL algal culture, 100-μL was mixed with 900-µL dilution buffer. From this dilution, 10-μL was mixed with 10-μL of Trypan Blue, and was transferred to a hemocytometer counting chamber. Following the same lab rules in the video, determine the percent cell viability if all 5 squares had the same count as the square below (show your solution): 2. Determine the concentration of the original 100-mL algal culture in cells/mL (show your solution). 3. Research on what DAPI staining is and discuss its current applications in cell biology experiments.

ACTIVITY NO. 14: ASSAYS OF ANTITHROMBIN TEST 1. Illustrate the process behind Antithrombin activity assay. Write the interpretation of results. 2. Illustrate the process behind Antithrombin antigen level test. Write the interpretation of results. 3. Explain the mechanism of the following etiologies of acquired antithrombin (AT) deficiency. A. DIC B. Nephrotic syndrome C. Heparin therapy D. Liver dysfunction

Need help fast Q6) Why would a sample be over 100%? Do you think 110% accurate is the same as 90% accurate? Q1) Why are there 2 different ‘stops’ on the micropipette? Q2) There’s many mistakes you can make micropipetting. Describe 3 of themQ4) What does a P100 pipette mean? P20?

Activity 3: Short Essay Directions: Choose two questions to answer. You may use a separate sheet to complete the task. 1. Ascorbic acid (Vitamin C) is a soluble vitamin in the human body. Justify your answer. 2. Why can termites eat wood barks and other thick and hard parts of plants? Explain your answer. 3. Is using steroids good in the human body? Why or why not? 4. What is the difference between DNA and RNA?

TASK No 2 MICROCIRCULATION A patient, 50 years old, suffers of chronic pyelonephritis and massive proteinuria (loss of protein in the urine). On examination: edema on the face and legs is noted. Questions: 1. To which group of disorders of microcirculation is edema refered? 2. What other disorders of microcirculation are related to this group? 3. Indicate the cause of edema developmen in the patient. 4. Indicate the mechanism of correlation between the loss of plasma proteins and the development of edema? 5. List the pathogenetic factors of edema formation. 6. What are the possible causes of the lymphatic insufficiency? 7. Indicate the biological significance of microcirculation disorders.

TASK N 2 A group of patients entered the hospital unconscious. All patients are employees of a chemical plant, where mass poisoning with cyanide compounds occurred. Questions: 4. Describe the mechanism of formation of this type of TPP. 5. List the changes in blood gas composition and pH at this form of TPP. 6. How can you explain these changes in blood gas composition and pH? 7. What kind of tissue cell death is typical in this case?

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Adaptive Learning by PrepU Question 2 of 5 A 3-year-old child is to receive 500 ml of dextrose 5% in normal saline (D5NSS) solution over 8 hours. At what rate (in milliliters/hour) would the nurse set the infusion pump? Record your answer using one decimal place.

est on the Prac X pps Bb Reassessments X Bb About this Modi X * Question Completion Status: QUESTION 6 Secure https://blackboard.le.ac.uk/webapps/assessment/take/launch.jsp?course_assessment_id=_39582_1&course_id=_41417_1&content_id= Iv Bag, Medical Palmar Oceanfron... Glv Bag, Medical Dr... Amazing Opportu... Unavailable in you... COO CH₂ CH-COO- 21 HO-C-H S COO- Isocitrate 4 OL AG = -8.4kJ/mol AG' = -17kJ/mol NAD Bb About this Modi X Bb Practical report X esc NADH 2 + H+ You want to measure the activity of isocitrate dehydrogenase from a tissue sample by following the production of NADH. Which of the following components would you need to include in your assay mixture? a. Water b. Bicarbonate solution c. Phosphate buffer solution (pH7.5) d. a-ketoglutarate solution e. NADH solution f. NAD solution g. Isocitrate dehydrogenase mixture Oh. Isocitrate solution F2 CO Click Save and Submit to save and submit. Click Save All Answers to save all answers. QUESTION 7 0.25mL of a stock solution…

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EXERCISE REVIEW SHEET 5 Classification of Tissues Name Lab Time/Date Tissue Structure and Function: General Review 1. Define tissue: 2. Use the key choices to identify the major tissue types described below. (Some choices may be used more than once.) Key: connective epithelium muscular nervous 1. lines body cavities and covers the body's external surface 2. pumps blood, flushes urine out of the body, allows one to swing a bat 3. transmits waves of excitation 4. anchors and packages body organs 5. cells may absorb, protect, or form a filtering membrane 6. most involved in regulating body functions quickly 7. major function is to contract 8. the most durable tissue type 9. abundant nonliving extracellular matrix 10. forms nerves Epithelial Tissue 3. On what bases are epithelial tissues classified? 4. How is the function of an epithelium reflected in its arrangement? 5. Where is ciliated epithelium found? What role does it play? 6. Transitional epithelium is actually stratified squamous…

Learning Task 16-03 - Antibiotic Effectiveness PART A • Record the steps that the laboratory technician follows in the video. • Highlight, by colour or bolding, the steps that ensure the experiment is sterile. Materials You will need: 6 millimeters filter paper disks, sterile Petri dishes, agar plates and no added antibiotics, sensitive and resistant bacterial cultures, an antibiotic to test and antibiotics control Steps 1. Pick colony and grow overnight 2. Dilute culture and grow to OD = 0.5 3. Prepare antibiotic stock 4. Add antibiotic to each disc 5. Dry discs at room temperature 6. Spread bacteria evenly on agar plate 7. Place discs in center of sectioned areas 8. Incubate plates 16-20h and measure zones PART B Re-watch the video and explain why the following steps are necessary: • Why use tweezers to put disks on the plate? • Why sterilize tweezers after each disk is put on the agar? • Why spread the bacteria out all over the agar plate? • Why put a disk with NO antibiotic on each…

Quiz: Ch 14-16 1 pts Question 1 Directions: Calculate the following dosage using the ratio and proportion method. Round mL answers to the nearest tenth unless otherwise indicated. Order: Amoxil 0.5 g p.o. q6h Available: Amoxil capsules labeled 250 mg per capsule Question 2 1 pts Directions: Calculate the following dosage using the ratio and proportion method. Round mL answers to the nearest tenth unless otherwise indicated. Order: Raloxifene HCI 60 mg p.o. b.i.d. Available: Raloxifene HCI tablets labeled 60 mg per tablet

Need help with questions 5) Given your review of the Gram Stain and the structural basis for how it works, why would a cell wall synthesis inhibitor be more effective for a Gram-positive cell type? 6) Given your knowledge of molecular biological principles, why would a protein synthesis inhibitor work with both cell types? Why might one protein synthesis inhibitor be more effective than another? For the second part - Hint: Think of change....what might have changed? 7) Given your knowledge of molecular biological principles, why are metabolic inhibitors not effective? Hint: Think of change....what might have changed? 8) A Gram-negative infection has been determined in a patient, what antibiotic would you suggest for treatment and why?

Questions: 1- Name the different type of dialyzers. 2- What is relationship between hollow fiber pore size and membrane flux in a dialyzer? 3- Explain the pressure gradient across the semi-permeable membrane in a dialyzer?

topic is about microtomes

LAB ACTIVITY 5.1 1. What might happen if the tourniquet is left for more than two minutes? How about if the tourniquet is tied too tight or too close to venipuncture site? 2. Why is collecting blood in the foot prohibited for patients with diabetes mellitus LABORATORY ACTIVITY 5.2 1. Give the advantages and disadvantages of using the “open system” and “closed system” in venipuncture. 2. Why are specimens for bilirubin testing protected from light? Discuss handling procedures for bilirubin samples. LABORATORY ACTIVITY 5.3 1. What are the consequences if an EDTA tube is filled first before a red top tube? 2. What must be done if an admitted patient refuses to blood extraction? LABORATORY ACTIVITY 5.4 1. Why should we not label tubes prior to blood collection? 2. Compare and contrast “NPO” from “fasting”. LABORATORY ACTIVITY 5.5 1. Why must a discard tube should be collected first before an anticoagulated tube when using a “butterfly” to collect blood? 2. What is “EMLA”? Give its purpose.

/Coursework 2 Remaining Time: 04:30:04 You have cultured some cells for a flow cytometry experiment. The cell concentration has been calculated using an automated cell counter as 1.5 x 10° cells/mL, and the overall viability of the cells is 90%. You collect your cells, wash them and resuspend in a final volume of 1.5 mL of FACS buffer. Calculate the concentration of viable cells per mL in your resuspended sample giving your answer to 3 decimal places [ Number x 108 cells/mL. You receive a second cell sample from another researcher to include in your experiment. These cells are resuspended at a concentration of 2 x 10 cells/mL. For each sample you run on the flow cytometer you require 250,000 cells. Calculate the volume of the cell suspension that you need to add to each FACS tube. Give your answer in microlitres (L) to 1 decimal place, Number AL You have made the flow cytometry sample up to a final volume of 250μL and now need to label your sample with an antibody. The antibody stock…

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Test: Quiz 5: X Cell membrane fl X = 9.1 Signaling Mol X * Question Completion Status: ard.com/webapps/assessment/take/launch.jsp?course_assessment_id=_407798_1&course_id=_156261_1&content_id=_12333940_1&step=null a. Increasing the interaction with water molecules O e. only making them less fluid QUESTION 6 Bb Microsoft Word O d. Cl- O e. Na+ Since membranes are selectively permeable, which of these would be able to get through without any help? O a. glucose O b. methionine O c. molecular oxygen (02) - O c. polar molecules in place of three fatty acids O d. a phosphate group in place of a carbon in glycerol O e. none of the above X Bb Serial Dilution We x Bb Molarity Workshe X QUESTION 7 Compared to a triglygeride, a phospholipid has a. a phosphate group and polar molecule in place of one fatty acid O b. phosphate groups in place of two fatty acids O X Click Save and Submit to save and submit. Click Save All Answers to save all answers. hp K 6000 DO 0.8 ● W **** | M MyOpe D DE ** ****…

Must asnwer both questions   4. How would you create a 200µl stock solution that is 1/10th the dilution of the original solution? Your base solution is PBS buffer. 5. If you wanted to test the effect of a 1:2000 dilution (2,000-fold) of lemon juice on PtK2 cells, how much of a 1:10 working stock of lemon juice would you add to the 6mls in an experimental flask?

Name: Jictorn Zunter Pre-Lab Questions 1. An unknown solution turned a strip of red litmus paper blue. Is this solution acidic or basic? basic 2. Which solution: 0.20 M NaOH or 0.20 M HCI, would you use to titrate an unknown solution that turned blue litmus paper red? 3. A burette had an initial volume of 5.2 mL and a final volume of 32.6 mL, what is the amount of solution from the burette that was used? Show you calculation below. Vz-V,=32.c6-5.ZML =27.4mL 4. 25.0 mL of an unknown acidic solution was titrated with 42.7 mL of a 0.15 M NaOH solution. What is the M of the unknown acid? Show your calculation below. 5. What is the Normality of 0.25 M H2SO4 6. What is the N of 0.45 M Mg(OH)2 7. Using the formulas provided for you in the lab introduction, fill the missing areas in the following table РОН 8.5 pH [H'1 [OH] 3.7 3.2x108 1.7x103 Show 1 row of calculations, below:

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ACTIVITY NO. 15: LUPUS ANTICOAGULANTS 1. What are the clinical criteria for a patient to undergo tests for lupus anticoagulant? 2. Illustrate the steps of mixing studies specified for lupus anticoagulant. 3. Illustrate the steps of parallel assays for lupus anticoagulant.

TASK No 2 In a patient of 60 years old after the surgical removal of stomach cancer and subsequent treatment, an enlarged lymph node in the left supraclavicular area was detected. Atypical cells, which were similar to the cells of a previously removed tumor, were found after the biopsy of this lymph node, Questions: 4) What is the name of tumor cells spreading throughout the body? 5) What is the difference between tumor cells and normal cells?