Lab 2 Assignment Fall23

.docx

School

University of Nebraska, Omaha *

*We aren’t endorsed by this school

Course

1450

Subject

Biology

Date

Dec 6, 2023

Type

docx

Pages

Uploaded by ChiefSteelCapybara35

Lab 2 Assignment Measurements, Units, and Laboratory Safety Scientists collect data in the laboratory or in the field and then present that data in a professional format. For each lab assignment, you will rewrite your raw laboratory exercises and the data you collect and answer the homework questions. Use the rewrite to review the objectives of each exercise which will help you answer the homework questions. All questions must be answered for full points. Instructions: Insert your answers from your lab work and your data into this electronic file. Redraw your graphs using the excel template and paste them into the lab. Answer the questions at the end. Upload the assignment prior to the beginning of your lab section in week 3. Make sure you give yourself time to troubleshoot any issues you may have with uploading the assignment. You are responsible for uploading the correct file. Part 2. Scientific Units Commonly Encountered in Biology CHEMICAL AMOUNT OF A SUBSTANCE If sodium chloride (NaCl, table salt) has a molecular mass of 58.44 Da, a) what is the mass in grams of three moles? _________ b) of half a mole? _________ c) of a millimole (one-thousandth of a mole)? _________ CONVERTING BETWEEN METRIC UNITS 1. An average chicken egg weighs 57 grams. How much is this in mg? ___________________ 2. One ml of blood (equal to one cc or one cubic centimeter) was drawn from the squirrel. How many liters of blood would this be? ___________________ 3. A blue whale can be up to 30.5 meters head to tail. How long would this be in kilometers? __________ 4. The bacteria cultured from a wound was 1 µm in diameter. How big is this bacteria in nanometers?_________ © UNO Biology Department 2023. Do not submit for posting on websites.

Part 3. Micropipettes: Use and Accurate Volume Measurement PREPARATION OF DILUTIONS FOR SUBSEQUENT WORKS 4b. Determine how much water and how much 0.1 mM dye must be combined to make the following solutions: 0.1 mM Dye (125 or 250 mL Flask) Water Dilution (50 mL Flask) ______________ mL + _______ mL = 10 mL of 0.05 mM dye ______________ mL + _______ mL = 10 mL of 0.01 mM dye Part 4. The Spectrophotometer as a Tool for Quantitative Measurement DOES A “CLEAR” CUVETTE HAVE ABSORBANCE PROPERTIES? Select the graph and use the “Chart Tools” (right click) to select “Edit Data.” Enter your data into the spreadsheet and the graph will update with your data. PC Users: Edit Excel Data is under the “Design” tab in Word 2010. Use the “Help” function to find the tool if you have a different version. 0 2 4 6 8 10 12 Absorbance Spectrum of Cuvette Wavelength (nm) Absorbance (A) © UNO Biology Department 2023. Do not submit for posting on websites.

ABSORBANCE PROPERTIES OF AN ORGANIC DYE IN AQUEOUS SOLUTION You measured the absorbance of a dilute solution of indigotine at the same intervals across the spectrum and you blanked the spectrophotometer at each wavelength prior to measuring the absorbance of the dye sample . Why is this necessary for accurate measurement of the absorbance of dye? Select the graph below and use the “Chart Tools” (right click) to select “Edit Data.” Enter your data into the spreadsheet and the graph will update with your data. PC Users: Edit Excel Data is under the “Design” tab in Word 2010. Use the “Help” function to find the tool if you have a different version. 0 2 4 6 8 10 12 Absorbance Spectrum of Indigotine Wavelength Absorbance Based on this spectrum, which wavelength(s) was most useful for monitoring indigotine in solution? ___nm. ESTIMATION OF DYE CONCENTRATION USING THE SPECTROPHOTOMETER Select the graph below and use the “Chart Tools” (right click) to select “Edit Data.” Enter your data into the spreadsheet and the graph will update with your data. PC Users: Edit Excel Data is under the “Design” tab in Word 2010. Use the “Help” function to find the tool if you have a different version. © UNO Biology Department 2023. Do not submit for posting on websites.

Your preview ends here

Eager to read complete document? Join bartleby learn and gain access to the full version

Access to all documents
Unlimited textbook solutions
24/7 expert homework help

Related Questions

Explain the following areas about (ultrasound device) 1. What are the components of the machine? 2. Working principle 3. What are the common malfunctions ((ultrasound device)) that a medical device engineer should know 4. Conclusion
Prior to each use of an N95, the healthcare professional should perform a fit test. put on gloves. do a seal check. wash her/his hand.
This is homework for a practice assessment. I need some assistance with these questions, so I can use them for studying material. Thank you so much! I highly appreciate it!

Recommended textbooks for you

Comprehensive Medical Terminology
Nursing
ISBN:9781133478850
Author:Jones
Publisher:Cengage
Case Studies In Health Information Management
Biology
ISBN:9781337676908
Author:SCHNERING
Publisher:Cengage
Complete Textbook Of Phlebotomy
Biology
ISBN:9781337464314
Author:Hoeltke
Publisher:Cengage
Surgical Tech For Surgical Tech Pos Care
Health & Nutrition
ISBN:9781337648868
Author:Association
Publisher:Cengage
Understanding Health Insurance: A Guide to Billin...
Health & Nutrition
ISBN:9781337679480
Author:GREEN
Publisher:Cengage
Biomedical Instrumentation Systems
Chemistry
ISBN:9781133478294
Author:Chatterjee
Publisher:Cengage