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Hi, hope you're doing great! I need help with a question, I have screenshot  thank you!

please solve this step by step calculations

Please help me ??? very urgently need to submit and the question is complete please help ASAP will appreciate your help?

Theoretical Data Following the modified protocol for the isolation of Escherichia coli bacteriophage of Encabo (2018) presented below, compute for the pfu/ml of the chloroform-treated lysate. 1ml 1ml 1ml 1ml 1 Chloroform-treated lysate 1ml Dilution 10-3 10-4 10-5 0 0 0 0 0 9ml 9ml 9ml 10-1 10-² 10-3 Empty sterile tubes Incubate inside ref for 15-20min + 0.1 ml lysate-E.coli mix 0.1 ml 9ml 9ml 104 10.5 // 0.1 ml 0.1 ml +0.5 ml E. coli ↓↓↓↓↓↓ Incubate O/N at 35°C, observe for plaques Molten soft agar overlay B. Quantification of the Infective E. coli other Bacteriophage Particles in the Raw Sewage Sample Volume of Stock Plated (in ml) Bottom agar No. of Plaque-Forming Units (pfu) Ave. A B 254 265 132 110 11 23

I am not understanding why TA of alcohol precipitation is used as TA of purified protein. I provided 2 photos, 1 with the questions and the correct answer, and the other photo is a screenshot of the explanation.

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D Question 9 An enzyme assay of 2 mL of extract shows 7.3 U/mL and a biuret assay shows 4 mg/mL protein on the initial sample. It is salted out and the pellet is washed and resuspended in 0.4 mL buffer. It is re-assayed and shows 12.1 U/mL activity and 1.3 mg/mL protein. What is the change in fold purity for this sample? (answer to 2 decimal places)

Calculate these for 1 ug of plasmid DNA in a final total volume of 20 ul. All enzymes being used here are at a concentration of 10 U/µL. For double digests, give the appropriate volume for each enzyme and decide which buffer to use. (HINT: look up double digest conditions on the NEB website.) You should always calculate all volumes in advance to ensure the correct working concentrations and so that you can prepare the digests as efficiently as possible. It is a good idea to check off each component as it is added to the microfuge tube. Complete the table below, including the volume of DNA sample(s) you will need for 1 pg of the DNA (from two different methods in Experiment #3) based on the concentration(s) you determined from the OD260 value, which you will restriction digest with each of the restriction enzymes singly and in double digests in Part A. For example, the volume of DNA sample that contains 1 µg DNA for DNA sample from Part A, Experiment 3: OD260 = 0.024, dilution 500x…

d/e/1FAIpQLSfTle9UfP15_VUqFI-ACEQd1XBykXv5Lr4dEMQbLJ1d6fCupw/viewform Students subjected three samples of five different molecules to gel electrophoresis as shown in Figure 1 A B C DE +2 3 Wells 4 8. Which of the following statements best explains the pattern seen on the * gel with regard to the size and charge of molecules A and B? 1 point molecules A and B are positively charged, and molecule A is smaller than molecule B. molecules A and B are positively charged, and molecule A is larger than molecule B. molecules A and B are negatively charged, and molecule A is smaller than molecule B. molecules A and B are negatively charged, and molecule A is larger than molecule B. Sign out

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bio 111 intro to cell, i need help in my post lab assignment. read the question carefully please and if you have any questions

Hello. Please see the attached screenshot. Please demonstrate how the entropy score was calculated. Thank you.

WELL NUMBER ORGANISM 1 2 3 4 6 7 8 Human +++ +++ + tt t++ + ++ Cow Chimpanzee t t+ Frog Monkey オt+ ャ+ ++ KEY: +++ Heavy Agglutination (Gel Solidification) + Medium Agglutination (Thick Liquid) Slight Agglutination (Flecks) No Reaction Lab Questions: 1. Animals that are most closely related to humans exhibited ichimpanzee agglutination. Heavy Medium Slight No 2. Animals that show little relation to humans exhibited cow agglutination. Heavy Medium Slight No 3. Which animal is most closely related to humans? Cow Chimpanzee Frog Monkey 4. Which animal is most unrelated to humans? Cow Chimpanzee (Frog Monkey

Using question 2 you can answer question 3 which I need help with

1. Compute the volumes needed to complete this table. Stock Final Volume for Volume for 5x reaction Reaction Component Concentration Concentration 1x reaction sddH,0 up to 25 ul up to 25 ul Buffer (ViBuffer A) 10X 1X MgCl, 50 mM 3 mM DNTP 2 mM 100 uM forward primer 10 uM 0.2 uM reverse primer 10 uM 0.2 uM Taq polymerase 1 U/ ul 1 U/ 25 ul DNA template 1-2 ng 1 ul *5X reactions = 2 samples, 1 NTC, 1 positive control, 1 pipetting allowance

Droplet based single cell DNA sequencing uses a microfluidic platform which generates droplets encapsulating individual cells. A micrograph of such a device [Zilionis et al, Nature Protocols 2016] is presented in Figure Q3. The channels are 25 μm deep. (a) (b) Oil Figure Q3. Micrograph of the microfluidic droplet device. Scale bars are 100 µm. Adapted from Zilionis et al, Nature Protocols 2016. Red arrowheads show individual cells, and black arrows indicate flow direction. (c) RT/lysis reagents Cells Barcoding hydrogel beads A company wants to explore the design of a prototype of the device made from PDMS. Describe a suitable fabrication process, explaining the rationale behind each individual step. Schematics can be drawn to illustrate your response. You may want to refer to the datasheets at the end of the script. Testing of the prototype was successful and the company wants to explore the feasibility of manufacturing the device from glass. Describe the fabrication process for this…

Q4 (a) SDS-PAGE. Sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE) is used to identify the molecular weight of unknown protein samples. Briefly outline the principle of SDS-PAGE. Marks: 2 For the toolbar, press ALT+F10 (PC) or ALT+FN+F10 (Mac). BIUS E v E Y A ... Paragraph Arial 14px

Hey I am practicing for a molecular biology test and I am having trouble with is practice problem. I am not sure how to set it up to determine the amount of Acid Phosphatase. Can you help? Determine the amount of Acid Phosphatase in micrograms/ gram wheat germ from the following experimental values. The amount of acid phosphatase found in 198 ul of extract was 8 ug. The amount of extraction buffer used was 8 mL and the amount of wheat germ was 0.8 g.

Electrophoresis     A protein required 6.8 min to travel 82 cm to the detector in a 96 cm -long capillary tube with 25.4 kV between the ends. Find the apparent electrophoretic mobility.

what are the volume dilution and dilution factor for bacteriophage A，B，C

Cytokine Storm (Cytokine Storm) COVID-19The high mortality rate in COVID-19 patients is caused by the occurrence of a cytokine storm (cytokine storm) in the patient's body. This is based on research supported by data obtained, among others, from laboratory results; that there was a significant difference between patients who recovered and those who died. What research data? Please Answer these following structured questions; Are cytokines a protein? How many amino acid residues of human cytokines? The sequence of the amino acid residues? Accession number at GenBank ? Determine the mRNA sequence Determine the coding sequence Define the sequence template What is a Cytokine storm What research data support the occurrence of Cytokine storm in humans Answers on paper with source cited provided will be helpful since im new in this part and want to learn from the source you find the answer Thank you

How will the information required to describe your RFP be obtained? Which kinds of methods are you going to employ?    lab intro attached if needed