C-Elegans_project-lab

.docx

School

Brooklyn College, CUNY *

*We aren’t endorsed by this school

Course

1010

Subject

Biology

Date

Dec 6, 2023

Type

docx

Pages

4

Uploaded by bonu007

Report
C.Elegans Report Brian Fernandez SEC: T2CB Due Date: 10/11/23 Pre-Knowledge This experiment seeks to demonstrate the Mendelian character of genetics through the cross-reactivity of two sexes of C. elegans (HERMAPHODITES XX and XO). It is intended to generate offspring with inheritance patterns similar to those observed in Mendel's genetic study of peas, using worm husbandry. Punnett predicts can be applied to the genotypes and phenotypes of both genotypes and progeny, allowing for the analysis of Mendelian genotype and phenotype. The primary objective of the experiment is to demonstrate cross-reaction between two sexes of the species C. elegans and to yield offspring with Mendelian genetic inheritance patterns. Additionally, the cross-reaction data should indicate whether the mutation is X-dependent or autosomal (DPY-3). The hypothesis of the experiment is that this mutation is X dependent, however, dosage compensations may mask the dominant or recessive character of the gene. Introduction C. Elegans are unsegmented worms with elongated cylindrical bodies. They would measure around one millimeter in length. They also have translucent bodies allowing for simpler observation of their internal structure. In the lab, they are placed in agar plates, roaming freely within their borders alongside their food. These organisms have a variety of two sexes, being hermaphrodites and males. Like all other males, C. elegans worms produce sperm to reproduce/procreate. However, the hermaphrodites have both sperm
and oocytes. The hermaphrodites have the option to either self-fertilize or have the males fertilize. However, it’s been shown that male fertilization has better results in comparison to self-fertilization. The ratio between the X chromosome to the autosome is the determining factor in the outcome of sex for the. Hermaphrodites have two X chromosomes in them making the ratio of X to A one . Males on the other hand only have up to One X chromosome . This leads to their X to A ratio being 0.5 . What this would lead to is a population where there are fewer chances of males being born than hermaphrodites. The worms have been studied for their gene regulation. They have a short lifecycle along with a fast maturation rate. This makes them the optimal species for studying inheritance/ genetic patterns. Methodologies and Techniques To handle worms, group members had to be confident in their skills and most importantly competent. Hand-eye coordination played a major key in this experiment. First, the group members would see how to differentiate between the sexes of the worms. This was possible through the observation of the tale. The male tales would be a more dull and blunt-like tail. While the hermaphrodite would have a longer more narrow and sharp tale connected to its back. Along with this, the size was also a major part contributing to whether it was an adult or not. The first time transferring the worms included transferring of hermaphrodites that were him-8 and dpy-3 homozygous and the males him-8 homozygous. 2 hermaphrodites dpy-3 and him-8 homozygous were put in plain agar plate with food. They were left on this plate for a week. After the week the worms would be seen through the dissecting microscope and the new breed of worms would be classified under F1. The second time crossing the worms involved the crossing of the F1 class with the adult hermaphrodites. Again another week was given until the next screening of the worms. Results Gametes Xdpy-3 X
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
  • Access to all documents
  • Unlimited textbook solutions
  • 24/7 expert homework help