BSC324%20study%20problems%20II%20FALL%202023

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Dec 6, 2023

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1 Tayler Reed Group review and problem solving session II 50 points Principles of Genetics BSC324 Name: Tayler Reed Date: 10/23/2023 1. Draw a ribonucleotide with a purine base in the space provided below. 2. Briefly describe the difference between a ribonucletide triphosphate and a deoxyribonucleotide triphosphate and list at least four examples of how the cell uses these molecules. In a ribonucleotide triphosphate, the main sugar component is ribose while in a deoxyribonucleotide triphosphate, the main sugar component is deoxyribose. Examples: (1) dNTPs are used in PCR to expand the growing DNA strand. (2) dNTPs provide nucleotides to an “unzipped” strand of DNA using the template of a single side. (3) dNTPs are used in DNA damage repair. (4) to sustain DNA synthesis at the replication fork. 3. Briefly describe what it means to say that a double stranded DNA molecule is antiparallel. Antiparallel is when two strands of DNA are side by side, but they run in opposite directions from one another. The 5’ end of one strand lays against the 3’ end of the other strand. 4. Draw the corresponding complementary strand to the single stranded DNA molecule listed below (be sure to label 5’ and 3’ ends). 5’ AATGTAGCCTGTAGT 3’
2 Tayler Reed 3’ TTACATCGGACATCA 5’ 5. Describe the Messelson-Stahl experiment. Why does it demonstrate that DNA replication is semiconservative? They cultured bacteria by adding nitrogen isotopes at key periods of cell division in E. coli. This revealed a pattern of incorporation of nucleotides in the replication of DNA. This pattern suggested that DNA replication is semiconservative, meaning 2 daughter strands are produced after DNA replication: both of the strands with one parental strand and one new strand. 6. Briefly describe the structure of the nucleosome. Why did nucleosomes evolve? A nucleosome is a thread of DNA wrapped around a histone, which is the core of a nucleosome. Nucleosomes compact into chromatin fibers. These nucleosomes fold in on each other to form a chromosome. It is suggested that nucleosomes evolved with eukaryotes and the packaging of these into chromosomes. 7. You are a deep sea explorer looking for new microorganisms living under the extreme conditions of the sea bottom. There you make a stunning discovery, a new bacterium with polymerases that can only synthesize DNA in the 3´ to 5´ direction. Everything else about its biology is similar to other bacteria. In honor of this unusual polymerase, and as a result of your poor performance in Latin class, you name your discovery Extremobacter reversicus . Based on what you know about more typical DNA polymerases what would you expect the error rate for DNA replication in Extremobacter reversicus to be compared to other living organisms? Explain. The error rate for DNA replication would be higher in Extremobacter reversicus . The higher error rate is due to the new species having no 5’-3’ exonuclease activity, which impacts DNA replication negatively. 8. In examining DNA replication using autoradiographs and a powerful microscope you obtain the following image of a replicating bacterial chromosome. Based on what you know about DNA replication explain the parts of the image denoted by the arrows.
3 Tayler Reed The arrows indicate the places where the replication forks are open to begin bacterial chromosome replication. There is a parent strand and a replicated daughter strand. 9. List the major components of the replisome and their functions. Helicase: unwinds DNA at the replication fork Single-Strand Binding Proteins: keeps replication fork open & binds and stabilizes single-stranded DNA Gyrase cleaves & switches duplex to release mechanical stress of unwinding DNA Polymerase Complex: makes strands of DNA (adds onto 3’ end) Primosome: RNA primer initiates new-strand synthesis DNA Ligase: joins Okazaki-fragments on lagging strand 10. Briefly describe some of the major differences between replication of bacterial versus eukaryotic chromosomes. Eukaryotic organisms have many origins of DNA replication while bacterial organisms only have one origin of replication. Eukaryotic chromosomes replicate bidirectionally while bacterial chromosomes are replicated in one direction. Eukaryotes: On the lagging strand, RNA primers are removed by enzymes and replaced with DNA Bacteria: primers are removed by the exonuclease activity by the DNA polymerase and the gaps are filled in with DNA
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