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Tayler Reed
Group review and problem solving session II 50 points
Principles of Genetics BSC324
Name: Tayler Reed
Date: 10/23/2023
1.
Draw a ribonucleotide with a purine base in the space provided below.
2.
Briefly describe the difference between a ribonucletide triphosphate and a deoxyribonucleotide triphosphate and list at least four examples of how the cell uses these molecules.
In a ribonucleotide triphosphate, the main sugar component is ribose while in a deoxyribonucleotide triphosphate, the main sugar component is deoxyribose.
Examples: (1) dNTPs are used in PCR to expand the growing DNA strand. (2) dNTPs provide nucleotides to an “unzipped” strand of DNA using the template of a single side. (3) dNTPs are used in DNA damage repair. (4) to sustain DNA synthesis at the replication fork. 3.
Briefly describe what it means to say that a double stranded DNA molecule is antiparallel.
Antiparallel is when two strands of DNA are side by side, but they run in opposite directions from one another. The 5’ end of one strand lays against the 3’ end of the other strand. 4.
Draw the corresponding complementary strand to the single stranded DNA molecule listed below (be sure to label 5’ and 3’ ends).
5’ AATGTAGCCTGTAGT 3’
2
Tayler Reed
3’ TTACATCGGACATCA 5’ 5.
Describe the Messelson-Stahl experiment. Why does it demonstrate that DNA replication is semiconservative? They cultured bacteria by adding nitrogen isotopes at key periods of cell division in E. coli. This revealed a pattern of incorporation of nucleotides in the replication of DNA. This pattern suggested that DNA replication is semiconservative, meaning 2 daughter strands are produced after DNA replication: both of the strands with one parental strand and one new strand. 6.
Briefly describe the structure of the nucleosome. Why did nucleosomes evolve?
A nucleosome is a thread of DNA wrapped around a histone, which is the core of a nucleosome. Nucleosomes compact into chromatin fibers. These nucleosomes fold in on each other to form a chromosome. It is suggested that nucleosomes evolved with eukaryotes and the packaging of these into chromosomes. 7.
You are a deep sea explorer looking for new microorganisms living under the extreme conditions of the sea bottom. There you make a stunning discovery, a new bacterium with polymerases that can only synthesize DNA in the 3´ to 5´ direction. Everything else about its biology is similar to other bacteria. In honor of this unusual polymerase, and as a result of your poor performance in Latin class, you name your discovery Extremobacter reversicus
. Based on what you know about more typical DNA polymerases what would you expect the error rate for DNA replication in Extremobacter reversicus
to be compared to other living organisms? Explain.
The error rate for DNA replication would be higher in Extremobacter reversicus
. The
higher error rate is due to the new species having no 5’-3’ exonuclease activity, which impacts DNA replication negatively. 8.
In examining DNA replication using autoradiographs and a powerful microscope you obtain the following image of a replicating bacterial chromosome. Based on what you know about DNA replication explain the parts of the image denoted by the arrows.
3
Tayler Reed
The arrows indicate the places where the replication forks are open to begin bacterial chromosome replication. There is a parent strand and a replicated daughter strand. 9.
List the major components of the replisome and their functions.
Helicase: unwinds DNA at the replication fork
Single-Strand Binding Proteins: keeps replication fork open & binds and stabilizes single-stranded DNA
Gyrase cleaves & switches duplex to release mechanical stress of unwinding DNA Polymerase Complex: makes strands of DNA (adds onto 3’ end) Primosome: RNA primer initiates new-strand synthesis
DNA Ligase: joins Okazaki-fragments on lagging strand 10.
Briefly describe some of the major differences between replication of bacterial versus eukaryotic chromosomes.
Eukaryotic organisms have many origins of DNA replication while bacterial organisms only have one origin of replication. Eukaryotic chromosomes replicate bidirectionally while bacterial chromosomes are replicated in one direction. Eukaryotes: On the lagging strand, RNA primers are removed by enzymes and replaced with DNA Bacteria: primers are removed by the exonuclease activity by the DNA polymerase and the gaps are filled in with DNA
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Related Questions
Molecular Biology (Biol-L211)
Dr. Nole
Central Dogma Practice - Processes
The general flow of genetic information is diagrammed below. Think carefully about what type of
molecule is represented by each item in the diagram and clearly address each of the following.
A. Label each structure as mature mRNA, pre-mRNA, protein, or DNA.
B. Label each arrow to indicate which is processing, transcription, replication, and translation.
C.
Identify the general location (on the appropriate molecule) of the promoter sequence and the
terminator sequence.
D. Identify the specific location of the place where the start codon and stop codon function most
directly (i.e., which molecule is actually translated?).
E.
Where does RNA polymerase bind to begin transcription?
F. Where specifically does the ribosome bind to begin translation-i.e., what are the ribosome binding
sites (in both prokaryotes and eukaryotes) and where are they found?
G. Label each end of the mature mRNA and the polypeptide to correctly…
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practice problem genetics
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ACTIVITY 7.3.2
1. Name differences between replication and transcription.
2. Write the sequence of the RNA transcribed from the following
DNA:
5' GATCAATGCTAG 3'
3' CTAGTTACGATC 5'
152 Copyright 2019. All Rights Reserved.
arrow_forward
Part II: Information Transfer
Background Information - Key Points
The background information provided for this lab has given you a general overview of some of 24
the key terms and definitions necessary to understand the transfer of information from gene to
protein. The information included below will help you work through the specific problems
included in your Tutorial 4 Assignment. When working on the problems remember the base mu to
pairing rules (Table 3).
Table 3: Rules for nucleotide base pairing.
cytosine (C) - guanine (G)
adenine (A)- thymine (T)
DNA
RNA
For Transcription:
●
●
●
●
cytosine (C) - guanine (G)
adenine (A)- uracil (U)
Initiation is determined by the
recognition of the promoter
sequence in the DNA by the RNA
polymerase.
Stef
The transcription start site is
downstream of the promoter and is
designated as the +1 site.
Aspartic acid
Alanina
Valine
Arginine
Serine
Lysine
Asparagine
Glutamic
TEOPO|0C|AGUCAG|UC|AG/DCAG/3G/
CAGUC
UGU
A C
A
Threonine
G
Methionine
Isoleucine…
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Solve it asap... All parts otherwise I will downvote
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Third letter
UCAG
UCAGH CAC
First letter
Part 5: Coding Practice
1. Use this sequence of DNA to answer the following former test questions:
5'-- TTAATGGGACAGCTTGTGTAGAGG --3'
a. What is the complementary strand of DNA?
b. Using the complementary strand of DNA (your answer from part a) as the template
strand, what is the transcribed mRNA sequence?
C. What is the amino acid sequence translated from the strand of mRNA synthesized in
part b (use the genetic code below)?
Remember:
i. Start codon!
ii. Stop codon!
bac
ocent
Seond letter
UUU Phe
UAU Tyr
UGU
UGC
Cys
UUC
UCC
UAC
Ser
UAA Stop UGA Stop A
UAG Stop UGG Trp G
C.
UUA
UCA
UUG
Le
UCG
[
CAU ]
CAC
CUU
CGU
His
CUC
C
CGC
ne.
CCA
Arg
Pro
CUA
CAA
CGA
CCG
CAG Gin
CGG
CUG
AAU 1
AAC Asn
ACU
AGU
[
[
AUU
AUC Ile
AGC Ser
Thr
A
AUA
AGA
AGG
ACA
AAA
Arg
AUG Met ACG
AAG
GUU
GCU
GAU
GGU
Asp
GAC
GCC
GGC
GUC
Val
G GUA
GCA
Ala
GAA
GGA
Gly
GAG Glu
GGG
GUG
GCG
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justify the answer and explain your reasoning
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3.
Analyzing the Molecules of Life - Molecular Diagnostics
An mRNA that encodes a variant of a human protein is as follows (the mutation is highlighted in red):
CUUGUUAACAACUAAACGAACAAUCUUUGUUUUUCUUGUUUUAUUGCCACUAGUCUCUAG
UCAGUGUGUUAAUCUUACAACCAGAACUCAAUUACCCCCUGCAUACACUAAUUCUUUCAC
ACGUGGUGUUUAUUACCCUGACAAAGUUUUCAGAUCCUCAGUUUUACAUUCAACUCAGGA
CUUGUUCUUACCUUUCUUUUCCAAUGUUACUUGGUUCCAUGCUAUACAUGUCUCUGGUAA
RT-qPCR can be used to detect the variant (mutation).
(a)
In a few sentences or using a simple diagram/flowchart, describe the basic principle of RT-qPCR,
and show the sequence of a DNA primer that can be used to reverse transcribe the entire RNA molecule
as shown at 37 °C.
(b)
Design a pair of primers (DNA oligonucleotides) for detecting the mutation by qPCR. Assume that
the PCR reaction will be performed at 72 °C. Show the sequences of your primers and their estimated
Tm.
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Practice test
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Do all
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1. How many codons are there in the Original DNA sequence?
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Question Guide:
1. Name and write the structures of the purine and pyrimidine bases present in DNA.
Purine
Рyrimidine
Structures:
Purine
Pyrimidine
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Please help me answer the GENETIC CODE problems
1. Describe the expirement that allowed researchers to first identify the codon for a specific amino acid.
2. Which of the following statemts about the genetic code are true and which are false? Please correct each false statement.
a. Each codon is composed of four bases
b. Some amino acids are represented by more than one codon
c.All codon represent an amino acid
d. Each living species is thought to have its own unique genetic code.
e. The codon AUG at the beginning of a sequence is a signal for protein synthesis to begin at that codon.
f. It is not known if the code contains stop signals for protein synthesis.
Thank you very much for your help.
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Complete number five..
please explain
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2. How many codons are there in the mRNA?
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I would like you to solve question 12.
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8:52
Protein 2-10092015113649.pdf
https:api.schoology.comv1attachment169963839...
Name
Class
Date
Interpreting Diagrams: Understanding the Main Ideas
The Genetic Code (MRNA)
Lysine
Lysine
Asparagine
Asparagine
Arginine
Arginine
Serine
Serine
Isoleucine
Methionine
Isoleucine
Isoleucine
Threonine
Threonine
Threonine U
Threonine c
Glutamic acid Glycine
Glutamic acid Glycine
Aspartic acid Giycine
Aspartic acid Glycine
Valine
Valine
Valine
Valine
Alanine
Alanine
Alanine
Alanine
"Stop" codon "Stop" codon Leucine
Trytophan
Cysteine
Cysteine
Al
Gl
"Stop" codon
Tyrosine
Тугosine
Serine
Serine
Phenylalanine Serine
Phenylalanine Serine
Leucine
Glutamine
Giutamine
CHistidine
Histidine
Arginine
Arginine
Arginine
Arginine
Al
Leucine
Leucine
Leucine
Loucine
Proline
Proline
Proline
Proline
Icl
A
G
Second Base in Code Word
Use the information in the accompanying figure to complete the following
table. The first row has been completed to help you get started.
DNA codon
MRNA codon
IRNA Anticodon
Amino…
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I need help with a matching biology question:
This is a single stranded molecule that in Eukaryotes passes from the nucleus to the cytoplasm.
This makes up the ribosomal subunits and reads the mRNA.
They carry amino acids during the process of translation.
The structure is the site of translation.
This is the enzyme complex that is used to copy DNA during transcription
matched with:
ribosome
mRNA
rRNA
tRNA
RNA polymerase
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6. What codons ar e found in the mRNA for the two mutated DNA?
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help..
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Question in Image. Thank you!
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Practice HW
make a nucleotide sequence (Template strand and Coding strand) of at least 15 base pairs for each of these steps to show your understanding of how genetic information is stored and used within a cell make sure that there are start and stop positions and note the direction of the sequence--- 5’ to 3’ or 3’ to 5.
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Task 1: Compare the characteristics of DNA to RNA.
Think before you drop - This is an all or nothing type of question. Double check that you are happy with where you placed all of the options before you submit the knowledge
check.
DNA
RNA
No Answers Chosen
No Answers Chosen
DNA & RNA
No Answers Chosen
Possible answers
Phosphate group
| Cytosine
Adenine
Basic unit is the nucleotide
Single strand
Double helix
E Phosphate-sugar backbone
| Uracil
| Guanine
| Thymine
::::
::::
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10:14
Protein 5-10092015113503.pdf
https:api.schoology.comv1attachment169963838...
Name
Class
Date
RNA
(pages 146-148)
| SECTION REVIEW
In this section you were introduced to the
molecule that helps put the information in
DNA to use: ribonucleic acid, or RNA. ANA Iis
a nucleic acid that carries information from
DNA to the ribosomes, the organelles in
which proteins are made. RNA also carries
out the process by which proteins are assem-
bled from amino acids.
RNA is quite similar to DNA in structure.
However, there are some important differ-
ences. RNA is single-stranded; DNA is dou-
ble-stranded. RNA contains the sugar ribose;
DNA contains deoxyribose. And RNA has the
nitrogenous base uracil; DNA has thymine.
In this section you also learned about the
process of transcription. Transcription is t
process in which part of a DNA molecule is
used as a template for the synthesis of a com-
plementary strand of RNA. This process is
mediated by an enzyme called RNA poly-
merase. The strand of…
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Please also help with this one . I am not sure what to do when I encounter a STOP codon.
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11 da
152 Chapter 13
? QUESTION
mRNA CODONS RESPONSIBLE
FOR LINING UP EACH OF THE 20 AMINO ACIDS
Amino Acid
Alanine
Arginine
Asparagine
Aspartic Acid
Cysteine
Glutamic Acid
Glutamine
Chel
Glycine
Hietidling
Histidine
Isoleucine
Leucine
Lysine
Methionine
Phenylalanine
Proline
Serine
Threonine
Tryptophan
Tyrosine
Valine
mRNA Codons
GCU
AGA
AAU
GAU
Kayles Kauffen
UGU
GAA
CAA
GGU
CAU
AUU
CUU
AAA
AUG
UUU
CCU
UCU
ACU
UGG
UAU
GUA
Code-End of the
(anticodon) tRNA
There are 64 codons. Some amino acids have several mRNA codons.
There is, however, no overlap of codes.
1. You should be able to fill in the 3-letter "code-end" of the tRNA
molecules in the table above. Remember, in RNA A pairs with U, and
G pairs with C. There is no thymine. Fill in the table.
2. What is the one-gene-one-enzyme hypothesis?
4. Where are tRNA and mRNA made?
3. Which molecule copies the DNA message for building a particular
protein?
What is this process called?
5. How many different amino acids are there in living…
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Question:-
A mixed copolymer was synthesized using 2 parts Cytosine and 1 part Adenine and the resulting mRNA was used for translation. What is the probability of producing the following amino acids? NOTE: Answer in fractions only.
1. Threonine _____
2. Asparagine _____
3. Histidine _____
4. Lysine _____
5. Proline _____
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Just Question 4
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8:47
ll 5G% 4
Chapter 9 DNA worksheet 2022.d...
2. Write the mRNA transcript from the DNA template in question 1.
Remember your enzyme for RNA polymerase must add RNA nucleotides
starting with the 5' direction and moving to the 3' direction. So for the
MRNA copy the strand of DNA that is 3'-5')
3. Refer to the codon chart at the bottom of the page. Translate the
mRNA into a polypeptide using the following rules. (2 points)
Direction of translation of mRNA is from 5' to 3'.
1.
MRNA codons are read in groups of three nucleotides.
3.
Translation must begin at the START codon.
4.
the STOP codon terminates translation.
2.
THIRD
LETTER
SECOND LETTER
U
TulUUU Phe
UUC Phe
UUA Leu
UUG Leu
A
UGU Cys U
JUGC Cys C
UCU Ser UAU Tyr
UCC Ser UAC Tyr
UCA Ser UAA STOP UGA STOPA
UCG Ser UAG STOPUGG Tp G
CCUU Leu
CÚC Leu
CỦA Leu
CUG Leu
AJAUU Ile
AUC Ile
AUA Ile
AUG Met STARTACG ThrAAG Lys
GGUU Val
GUC Val
GUA Val
GUG Val
CCU Pro CAU His
CCC Pro CAC His
CCA Pro CAA Gln
CCG Pro CAG Gln
ACU ThrAAU Asn AGU…
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Help me on this ?
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Complete the following problems.
1. Draw a molecule of phosphatidyl serine using palmitic acid and linoleic (Δ9,12) acid
2. Consider the following coding strand sequence of DNA to a corresponding amino acid sequence. This represents the first amino acids to be translated. Underline the codon where translation will be initiated, and give the amino acid sequence of the peptide produced.
5’-CGATGCTCAAGTTTC-3’
3. Within the matrix of the mitochondria, the pH is approximately 6.8 ([H+] = 1.6 X 10-7), while it is 7.4 ([H+] = 4.0 X 10-8) in the intermembrane space outside the matrix. Calculate the free energy driving transport of hydrogen ions from the intermembrane space to the matrix. Remember to include both the concentration and the membrane potential factors.
4. Draw the adenosine-5’-diphosphate.
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Related Questions
- Molecular Biology (Biol-L211) Dr. Nole Central Dogma Practice - Processes The general flow of genetic information is diagrammed below. Think carefully about what type of molecule is represented by each item in the diagram and clearly address each of the following. A. Label each structure as mature mRNA, pre-mRNA, protein, or DNA. B. Label each arrow to indicate which is processing, transcription, replication, and translation. C. Identify the general location (on the appropriate molecule) of the promoter sequence and the terminator sequence. D. Identify the specific location of the place where the start codon and stop codon function most directly (i.e., which molecule is actually translated?). E. Where does RNA polymerase bind to begin transcription? F. Where specifically does the ribosome bind to begin translation-i.e., what are the ribosome binding sites (in both prokaryotes and eukaryotes) and where are they found? G. Label each end of the mature mRNA and the polypeptide to correctly…arrow_forwardpractice problem geneticsarrow_forwardACTIVITY 7.3.2 1. Name differences between replication and transcription. 2. Write the sequence of the RNA transcribed from the following DNA: 5' GATCAATGCTAG 3' 3' CTAGTTACGATC 5' 152 Copyright 2019. All Rights Reserved.arrow_forward
- Part II: Information Transfer Background Information - Key Points The background information provided for this lab has given you a general overview of some of 24 the key terms and definitions necessary to understand the transfer of information from gene to protein. The information included below will help you work through the specific problems included in your Tutorial 4 Assignment. When working on the problems remember the base mu to pairing rules (Table 3). Table 3: Rules for nucleotide base pairing. cytosine (C) - guanine (G) adenine (A)- thymine (T) DNA RNA For Transcription: ● ● ● ● cytosine (C) - guanine (G) adenine (A)- uracil (U) Initiation is determined by the recognition of the promoter sequence in the DNA by the RNA polymerase. Stef The transcription start site is downstream of the promoter and is designated as the +1 site. Aspartic acid Alanina Valine Arginine Serine Lysine Asparagine Glutamic TEOPO|0C|AGUCAG|UC|AG/DCAG/3G/ CAGUC UGU A C A Threonine G Methionine Isoleucine…arrow_forwardSolve it asap... All parts otherwise I will downvotearrow_forwardThird letter UCAG UCAGH CAC First letter Part 5: Coding Practice 1. Use this sequence of DNA to answer the following former test questions: 5'-- TTAATGGGACAGCTTGTGTAGAGG --3' a. What is the complementary strand of DNA? b. Using the complementary strand of DNA (your answer from part a) as the template strand, what is the transcribed mRNA sequence? C. What is the amino acid sequence translated from the strand of mRNA synthesized in part b (use the genetic code below)? Remember: i. Start codon! ii. Stop codon! bac ocent Seond letter UUU Phe UAU Tyr UGU UGC Cys UUC UCC UAC Ser UAA Stop UGA Stop A UAG Stop UGG Trp G C. UUA UCA UUG Le UCG [ CAU ] CAC CUU CGU His CUC C CGC ne. CCA Arg Pro CUA CAA CGA CCG CAG Gin CGG CUG AAU 1 AAC Asn ACU AGU [ [ AUU AUC Ile AGC Ser Thr A AUA AGA AGG ACA AAA Arg AUG Met ACG AAG GUU GCU GAU GGU Asp GAC GCC GGC GUC Val G GUA GCA Ala GAA GGA Gly GAG Glu GGG GUG GCGarrow_forward
- justify the answer and explain your reasoningarrow_forward3. Analyzing the Molecules of Life - Molecular Diagnostics An mRNA that encodes a variant of a human protein is as follows (the mutation is highlighted in red): CUUGUUAACAACUAAACGAACAAUCUUUGUUUUUCUUGUUUUAUUGCCACUAGUCUCUAG UCAGUGUGUUAAUCUUACAACCAGAACUCAAUUACCCCCUGCAUACACUAAUUCUUUCAC ACGUGGUGUUUAUUACCCUGACAAAGUUUUCAGAUCCUCAGUUUUACAUUCAACUCAGGA CUUGUUCUUACCUUUCUUUUCCAAUGUUACUUGGUUCCAUGCUAUACAUGUCUCUGGUAA RT-qPCR can be used to detect the variant (mutation). (a) In a few sentences or using a simple diagram/flowchart, describe the basic principle of RT-qPCR, and show the sequence of a DNA primer that can be used to reverse transcribe the entire RNA molecule as shown at 37 °C. (b) Design a pair of primers (DNA oligonucleotides) for detecting the mutation by qPCR. Assume that the PCR reaction will be performed at 72 °C. Show the sequences of your primers and their estimated Tm.arrow_forwardPractice testarrow_forward
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