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Module 6 Lab Report Cell Membranes, Diffusion and Osmosis Student Name: Cell Membrane: 1.
What is the primary molecule that makes up cell
membranes? Phospholipids 2.
Label the cell membrane diagram below:
A Hydrophobic tails B Glycoprotein C Peripheral protein D Protein Channel E Hydrophobic heads F Carbohydrate G Integral protein H Glycolipid I
Bilayer
3.
In module 5 you extracted DNA from strawberries using dish detergent, water, salt and
alcohol. Using
what you now know about cell membranes, what was the purpose of the
dish detergent in the
extraction buffer? It was to break down cell membranes. The way it breaks down the cell membranes is
because it is an amphiphilic molecule which means is has hydrophobic and hydrophilic characteristics. It
uses hydrophobic properties that allow it to break down the cell membrane while the hydrophilic end interacts with the water. 4.
Why did the detergent work so well in the extraction buffer? It works well because it breaks apart the cell membranes which makes for easy DNA extraction.
5.
Watch the video linked here: Diffusion of Dyes in Agar
Why did the red dye diffuse farther? The red dye may have a higher concentration gradient.
6.
Watch the video linked here: Comparing Diffusion of Dyes in Agar
a.
Why was Potassium permanganate and methylene blue run twice in the
experiment? To be a control variable and also ensure the results are accurate. b.
Of the dyes being tested, would you conclude they were larger or smaller in
molecular size? If we compare the dyes to the control, they have a higher molecular weight.
c.
Defend your answer to question b. They diffuse at a slower rate. 7.
What process was observed in the videos? Diffusion a.
How would the rates of that process change if the concentration of the agar the
dyes are in was
decreased? There would be faster diffusion because there is a steeper concentration gradient. b.
What if the agar concentration was increased? There would be a slower rate of diffusion because there is a reduced concentration gradient. 8.
Elodea is a plant found in freshwater environments that is commonly used in the laboratory to study photosynthesis, osmosis and diffusion. Using the terms of tonicity, determine if each of the cell samples
from elodea were in hypertonic, isotonic or hypotonic environments.
For the images above the first image is isotonic the second image is hypotonic and the last image is hypertonic Animal cells have similar reactions as plant cells in environments of different tonicities. Label the
pictures of red blood cells with the correct tonicity of the environment they were most likely in. Hypotonic isotonic Hypertonic 1.
What is the salt concentration of saline? (You may need to research that) According to usgs.gov the concentration of saline The salt concentration of a saline solution is 0.9%
a.
What is most likely to occur to the red blood cells of a patient if the saline
solution they were
being given through their IV was a 10% NaCl concentration? The red blood cells would shrink
because the solution would become hypertonic. b.
Why aren’t patients on IV treatments given pure water instead of saline? Because the water is hypotonic compared to the body fluids of the patient it can cause the cells to swell. c.
5% Glucose in Ringer’s solution is a combination of water, NaCl, Glucose and
other buffering solutes. What is the advantage of using a Ringer’s solution with
5% glucose over regular
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LAB EXERCISE 3A
A. Cell Membranes. In the square provided, draw a double layer of phospholipids with a large protein
embedded within it. Label the parts of this phospholipid bilayer that are partially charged (polar) and
uncharged (non-polar).
B. Anatomy of a Cell. Draw a "typical" cell in the space below (see lecture notes or the
labeled/unlabeled Images on Canvas). Sketch the following organelles within the cell and label them.
Plasma (Cell) Membrane
Nucleus
Ribosome
Rough Endoplasmic Reticulum
Smooth Endoplasmic Reticulum
Golgi Apparatus
Mitochondria
Lysosome
Cytoplasm
Microvilli
Cilia
A region of exocytosis
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What's More
eorig sm
Table Completion
Direction: Complete the table below by identifving the different functions of the
structural component of the cell membrane and its location. o
Location
Structural
Component of the
Cell Membrane
Functions
(in the cell
membrane)
1. Phospholipid
bilayer
2. Integral
protein
dulos toinv
3. Peripheral
protein
ilang
onid
bb bos
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Lecture 6-Worksheet
Part 1: Below are TWO ER bound proteins. The green region is indicating the ER transfer signals.
1. Can you label the start and stop transfer signals based on the rules we just went over?
2. Draw and label these proteins.
(1)
(2)
N
N
ER lumen
Part 2: Sketch the orientation of this protein as it would appear in the transport vesicle and plasma
membrane.
membrane-enclosed
organelle
(e.g., ER or Golgi)
vesicle
plasma
membrane
1₂:0.
0-9
?
CYTOSOL
extracellular fluid
165
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Cell Membrane and Cell Transport WebQuest
Part I: Cell Membranes
Go to the following website: www.biology4kids.com/ files/cell_membrane.html
1. How is the cell membrane similar to a plastic bag with tiny holes?
2. What two components make up the cell membrane?
a. What are their functions?
3. What is the fluid mosaic model?
4. Sketch a section of the cell membrane, showing both phospholipids and proteins.
Label your drawing.
5. Label the diagram of the phospholipid molecule below with the following terms:
hydrophilic head, hydrophobic tail
This diagram
shows a
Head
simplified
version of a
phospholipid
molecule.
Tails
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Work 1. The main structural components of the eukaryotic cell of the animal. Fill in the
table.
CELL ORGANELLE
FUNCTION
ROLE IN BASIC
PHYSIOLOGICAL PROCESSES
SMOOTH ENDOPLASMIC
RETICULUM
ROUGH ENDOPLASMIC
RETICULUM
MITOCHONDRIA
RIBOSOMES
LYSOSOMES
CYTOCENTRUM
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Course: SCI Biology
Unit: Unit 08: Cell Membrane
SCI Biology - Unit 08: Cell Mer
1) The process of moving a large substance into a cell by means of a vesicle.
OA.) Endocytosis
OB.) Exocytosis
Save
2) The process of moving a large substance out of a cell by means of a vesicle.
OA.) Exocytosis
R) Endorytosis
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CELL STRUCTURE AND FUNCTION – EUKARYOTES, PROKARYOTES AND VIRUSES-LABORATORY REPORT SHEET
ACTIVITY 2: CELL MEMBRANES, OSMOSIS AND DIFFUSION
Initial Volume (Vi)
(mL)
Final Volume (Vf)
(mL)
Change in Volume
(Vi – Vf)
(mL)
A
20% sucrose
20% sucrose
92.0 mL
94.0 mL
-2.0 mL
B
40% sucrose
20% sucrose
92.0 mL
92.0 mL
0.0 mL
C
20% sucrose
40% sucrose
92.0 mL
90.0 mL
2.0 mL
Treatment
Percent Change
A
-2%
B
0%
C
2%
Hypothesize which dialysis bags should gain, lose or stay the same in regards to volume. Explain.
Which solution is hypotonic, hypertonic and isotonic?
Explain what the change in volume of the dialysis tube indicated and describe what happened when the volume increased and when the volume decreased.
Explain the relationship between solute concentration and amount of free water or water available for movement.
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Question Completion Status:
QUESTION 19
Which of the following structures is NOT a part of the plasma membrane?
Glycoprotein
Integral protein
Cholesterol
Mitochondria
O phospholipid
QUESTION 20
Which of the following does not affect the rate of simple diffusion of a substance
Concentration gradient
Temperature
O Size of the molecule
O Distance
O Number of carrier proteins
Click Save and Submit to save and submit. Click Save All Answers to save all answers.
P Type here to search
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Mastering 7: Chapter 6
Chapter 6 Question 6
Drag the appropriate labels to their respective targets.
plasma
membrane
smooth ER
vacuole
microtubule
nucleolus
Golgi apparatus
nucleus
plasmodesma
microfilament
peroxisome
rough ER
ribosome
cell wall
nuclear pore
chloroplast mitochondrion
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06:33 t 42 A
Lysosome
63
Compilation of Biochemistry Laboratory Activities Prepared by
Page 4 of
Things to ponder:
1. What particular specimen was used in observing the plant cell using the virtual scope?
Specimen for animal cell?
2. The microscope used in this lab is not powerful enough to view every organelle.
Which organelle(s) can you see in the cheek cell?
3. Name two organelles that are not visible, but should be present in the cheek cell.
4. What cell structures are common to all types of cells?
5. What cell structures are present only in eukaryotic cell?
6. List the structures that are common in both plant and animal cells.
7. List the structures that make plant and animal cells different from each other.
Tabulate your answer.
Compilation of Blachemistry Laboratory Activities Prepared by
Page 5 of
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Which of the following statements correctly states the process shown in the diagram?
O The process labeled 1 shows how cells can easily move molecules with the concentration gradient using high energy molecules.
The process labeled 2 shows how cells move molecules against the concentration gradient without needing high…
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Oct 8, 2020 at 2:10 PM
NAME
DATE
PERIOD
Cell Membrane Discovery
Big Idea: Cells move materials in and out of their environment through the cell membrane in order to
maintain
(balance) or
(equal state).
Composition of the Cell Membrane & Functions:
The cell membrane is also called the
membrane and is made of a
phospholipid
The phospholipids have a
(water
attracting or polar) head and two
(water repelling or non-polar)
The head of a phospholipid is made of an alcohol and phosphate group,
while the tails are 2 chains of fatty acids. Phospholipids can move apart and allow water and
other
molecules to pass through into or out of the cell. This is known as simple
because it does not require
and the molecules are moving
from areas of
concentration to an area of
concentration.
1. LABEL a phospholipid bilayer coloring the heads red and the tails blue. 2. LABEL the structure that is hydrophobic
and hydrophilic.
PHOSPHOLIPID
molecule
membrane
Another type of…
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Answer the question please.
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PART B: Vesicular Transport
D
plasma
membrane
nuclear envelope
C
ENDOCYTOSIS
endoplasmic
reticulum
transport vesicles
EXOCYTOSIS
A
В
CYTOSOL
Golgi apparatus
Q-6. Choose from the following terms to label A, B, C, & D.
EXTRACELLULAR
SPACE
[Not all of the terms are used] cis-Golgi, early endosome,
late endosome, lysosome, medial cisterna, peroxisome, trans Golgi
A:
B:
C:
D:
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Tonicity3
:=
Homework • Unanswered
Suppose the circle represents a cell which is sitting in a solution. Compare
the concentration inside the cell with the concentration of the surrounding
solution to determine the tonicity of each.
1% sugar
10% sugar
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The solution is...
into/out of the cell at equal rates.
The cell is...
isotonic
out of the cell.
Water will move...
hypertonic
hypotonic
into the cell.
II
II
II
II
II
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A Cell Transport Study Guide
Amara Mohamad - Cell Transpo
A docs.google.com/presentation/d/1rTHSAC-61C2E8sLBESlplITDoyKOX5hXDpSNLDJ23TY/edit#slide-id.SLIDES API169621999 3
1 2
3
4 I 5
Hypertonic means that the environment
outside of the cell has a higher
concentration of solutes than the cell
itself. That will attract water molecules
from the cell leading to the shrinking of
the cell
water concentration is greater outside
the cell and the solute concentration is
higher inside the interior of the cell that
is hypertonic to the hypertonic
surroundings. The net flow of water is
into the cell
In an
conce
equal
is no
chang
place
will er
4. The tonicity of a solution is determined by the presence of solutes that (circle one) [ ca cannot ]easily pass the cell membrane.
5. In a HYPERTONIC solution, there is a (circle ore) ( higher | lower ] amount of solutes
ide
ecell relative
the inside of a cell.
6. In a HYPOTONIC solution, there is a (circle one) [ higher lower ] amount of solutes outside…
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Are those the correct answers?
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MULTIPLE CHOICE QUESTION
Which of the following is FALSE about the
components found in the plasma membrane?
Some membrane proteins have carbohydrate chains
attached to them that helps them aid in cell recognition.
Integral proteins are sometimes considered transport
proteins.
Cholesterol helps to maintain flexibility and stability.
The layers of phospholipids are arranged so that the heads
face inward (interacting with eachother) and the tails face
outward (interacting with the environment).
Rewatch
Submit
!!
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he cell
TOPIC 2.4 Plasma Membranes
ENDURING UNDERSTANDING ENE-2 Cells have membranes that allow them to establish and maintain internal environments that ere diferent
from their external environments.
LEARNING OBJECTIVE
ENE-2.A Desaribe the roles of each of the
components of the cell membrane in
maintaining the intemal environment of the cell.
Label the hydrophobic and hydrophilic
parts of one phospholipid.
There are three proteins embedded in the
membrane. Choose a color for
ESSENTIAL KNOWLEDGE
ENE-2.A.1 Phospholipids have both hydrophilic hydrophobic R groups
and hydrophobic regions. The hydrophilic
phosphate regions of the phospholipids are
oriented toward the aqueous external or
internal environments, while the hydrophobic
and hydrophilic R groups.
Color oode the regions of each protein.
Predict what would happen if a mutation
caused one of the hydrophobic R groups to
be switched with a hydrophilic R group.
Explain your thoughts:
fatty acid regions face each other within the…
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Please draw the figure with all the figure's visas by hand
Note:The drawing should look like a student drawing and not an expert drawing, please make the marking on the drawing complete and in a clear line away from mixing words
Note: Please, please, draw by hand only. I do not want any drawing or electronic image of the figure
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Instructions
The internal environment of a living cell is constantly changing, and yet the cell needs to maintain a certain balance
in order to survive. It needs to take in water, food, oxygen, and other materials. It needs to expel excess water,
wastes, and the substances it produces that other cells in the body may need.
About
To help maintain the stable internal environments, the cell membrane contains structures that regulate the
movement of…
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Related Questions
- LAB EXERCISE 3A A. Cell Membranes. In the square provided, draw a double layer of phospholipids with a large protein embedded within it. Label the parts of this phospholipid bilayer that are partially charged (polar) and uncharged (non-polar). B. Anatomy of a Cell. Draw a "typical" cell in the space below (see lecture notes or the labeled/unlabeled Images on Canvas). Sketch the following organelles within the cell and label them. Plasma (Cell) Membrane Nucleus Ribosome Rough Endoplasmic Reticulum Smooth Endoplasmic Reticulum Golgi Apparatus Mitochondria Lysosome Cytoplasm Microvilli Cilia A region of exocytosisarrow_forwardWhat's More eorig sm Table Completion Direction: Complete the table below by identifving the different functions of the structural component of the cell membrane and its location. o Location Structural Component of the Cell Membrane Functions (in the cell membrane) 1. Phospholipid bilayer 2. Integral protein dulos toinv 3. Peripheral protein ilang onid bb bosarrow_forwardLecture 6-Worksheet Part 1: Below are TWO ER bound proteins. The green region is indicating the ER transfer signals. 1. Can you label the start and stop transfer signals based on the rules we just went over? 2. Draw and label these proteins. (1) (2) N N ER lumen Part 2: Sketch the orientation of this protein as it would appear in the transport vesicle and plasma membrane. membrane-enclosed organelle (e.g., ER or Golgi) vesicle plasma membrane 1₂:0. 0-9 ? CYTOSOL extracellular fluid 165arrow_forward
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- Explainarrow_forwardCan you please help me matchingarrow_forwardCourse: SCI Biology Unit: Unit 08: Cell Membrane SCI Biology - Unit 08: Cell Mer 1) The process of moving a large substance into a cell by means of a vesicle. OA.) Endocytosis OB.) Exocytosis Save 2) The process of moving a large substance out of a cell by means of a vesicle. OA.) Exocytosis R) Endorytosisarrow_forward
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