Lab 6

I need the solution of the second picture please

The following strip of chromatography paper has been placed in a polar solvent Which pigments the most polar?

I need help with my homework this question is very confusing How long would it take for a solution made by diluting 2.5 ml of #1 with 7.5 ml of H2O to react with 10 ml of #2?

Before and after for the image the color how is changed

Component#, Color, Distance component traveled (mm) Distance solvent front traveled (mm), Rf, Possible identity of component 000 Beta-carotene Chlorophyll a Chlorophyll b xanthophylls Figure 8. TLC chromatogram of the pigments (components) in spinach (example/reference). MS Figure 18. TLC of experimental spinach reference standard (1st, 3rd, and 5th columns) and two unknown plant leaves (2nd and 4th columns). MS Activity #5 (2 pts) Complete Table 5, Components of Unknown Plant Leaves. Use Figure 18 (above) to calculate the Rr value for each component in unknown leaf samples 1 and 2 (columns 2 and 4) using the method described above. Assign a possible identity of each pigment in leaf samples 1 and 2 (columns 2 and 4) based on comparison to the spinach reference standard. If the leaf samples contain pigments or spots that do not have comparable spots in the spinach standard, label these components as "unknown." Table 5. Components of Unknown Plant Leaves RKJC MS RKJC F Component# Color…

Most of the following conclusions regarding the photo shown above are correct Except: All of the following are correct regarding the disk diffusion test photos EXCEPT : Zone of inhibition Chlorine Chlorine Chlorine O-phenylphenol Hexachlorophene O-phenylphenol Hexachlorophene Hexachlorophene O-phenylpheno Quat Quat Quat Staphylococcus aureus (gram-positive) Escherichia coli Pseudomonas aeruginosa (gram-negative) (gram-negative) Select one: O to. Gram negative bacteria are the most sensitive to chemical agents O b. Only one of the four chemicals affected Pseudomonas O c. Gram positive bacteria are the most sensitive to disinfectants O d. Hexachlorophene was effective only against Gram positive bacteria. O and. Chlorine was effective against all bacteria bacteria tested

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Please answer both questions A and B. Question b needs drawing so clearly draw what is given. An illustration (IMAGE 2) can be use as guide

UV detection in liquid chromatography requires that the analyte have a

Biomaterial question: Give a brief and clear answer, please do not write by hand Question: Explain briefly the working principle and the information provided by the spectroscopic and chromatographic characterization techniques. Give an example to each of them.

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The figure above depicts an agar cube with a side length of 13\, \text{mm}13mm13, start text, m, m, end text. In an experiment, students submerged the cube in red dye for 121212 hours. The red dye permeated 1\, \text{mm}1mm1, start text, m, m, end text on each side, as indicated by the shading in the figure. Volume of a rectangular solid: V = lwhV=lwhV, equals, l, w, h Calculate the volume of the agar cube that remained unpenetrated by the red dye.

You are tasked to prepare 1000mL of 2 media preparations, however, instead of using a powder agar, you will be preparing the desired concentration from a prepared stock solution. Complete the information in the table and show your computation. Volume of Volume of diluent Desired Stock Solution stock sol'n needed to make Concentration needed 1000mL media 25% 12.5% 5% 1.5%

What are the processes of concentrate samples analysis in Uv-visible spectroscopy? Please shortly answer at your own words(3-4 lines).

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Please answer these 3 multiple choice

Paper Chromatography question 1. If there are two unknown samples yielded the same Rf value in the paper chromatography result, can we directly conclude that those two samples are identical? Explain briefly

Briefly discuss why increased chlorination of organic compounds increases the recalcitrance or stability of the molecule in the environment.

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What are the mechanisms of samples separation work in Thin layer Chromatography? Please shortly write at your own words. Answer should be to the point (5-6 lines maximum).

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All questions please.     1) What are the pigments and their applications: 2) What is the difference between lightfastness and permanence of pigments?3) Explain the tinting strength of pigments and its relation with the size of the particle.4) Explain the opacity of pigments and its relation with the size of the particle. 5) What are the 4 ingredients for paints and explain each in one sentence.       1) a) Draw a human eye in the space below (LARGE!), and clearly label each of the partslisted below. Iris aqueous humor ciliary muscles RetinaPupil Cornea vitreous humor FoveaSclera lens choroid optic Optic   b) In your diagram of the human eye, label all instances of refractions that happen fromwhen light first enters the eye to when it is absorbed by the retina.     2) Name 4 popular vision problem and explain each briefly.   3) a) Draw a schematic representation of a cone and a rod photoreceptors in the space below(LARGE!), and clearly label each of the parts listed at…

Sample contains (A, B, C, D) analyzed by thin layer chromatography using solvent system X the result showed: Rf Values: (A: 0.6, B: 0.8, C: 0.2, D: remained in the base line).1) If the sample was separated by using column chromatography. Which of these substances eluted first? And what is the detecting method? 2) If the sample was separated by using HPLC (1.Normal phase 2. Reversed phase) which RT of these substances comes first of both phases? And what is the meaning of RT? *

Question 2. After sterility test of an injectable it was observed that an injection is failed the test what will you do then depending on the product nature (heat sensitive/ heat resistant) (Not more than one sentence)? Mention the purpose of product inhibition test and how it is performed (Not more than one sentence) ? How will you check the validity of a medium (Not more than one sentence) ?

Question 4: Starting with a culture that contains 5.0 x 107 CFU/ml, describe a serial dilution scheme that would give a plate with 50 colonies.

A TCL was run with 5 subjected samples utilizing a silica plate and ethanol/chloroform in mobile phase. The measured distance are shown in the picture. 1. The most polar sample in the 5 subjects is? 2. The least polar sample in the 5 subjects is? 3. Find the Rf value of Sample C and B

What is Dry Flash Column Chromatography? Please explain at your own words( around 500 words).

briefly explain the principle behind this technique; affinity chromatography using a diagram to illustrate.

Explain how acetaminopen gives such spectroscopic and chromatographic graph by interpreting the peaks that appeared in the graphs. Fig1. Mass Spectrometric Chromatogram of Acetaminophen (the two graphs) Fig2. IR Spectrograph of Acetaminophen

Choose the one answer that fits best. Which of the following statements regarding the proper procedure for using Micropipettes is NOT correct? O a. You cannot use a 2-20 µl micropipette to pipet 200 µl O b. To expel all the liquid from the tip, you have to press the eject button O c. To draw up solution, press and hold the plunger at the first stop before entering the solution O d. Micropipettes always require the use of a disposable plastic tip O e. While pipetting, micropipettes should always be held as straight as possible

Answer the ff and state references: For his experiment, Brian needs to prepare 30 plates (use maximum volume), 15 slants (small test tube), and 15 stabs (big test tube) of Luria  What is the total volume of the medium needed? What is the amount of each component needed, given the following media composition per liter of distilled water: Component                                 g/L tryptone 10.0 NaCl 5.0 yeast extract 10.0 agar 15.0

From your results, which component had the highest Rf value and which had the lowest? Based on your answer from the previous question, what could be the reason behind some components having an Rf value higher than the others? Compare the qualities of the two components that rendered the highest and lowest Rf After performing the simulation, what do you think are the possible practical applications of chromatographic techniques that are relevant to your program?