FINAL LABS_BIOS251_Online Labs_Week 3 Membrane Transport Lab (1)
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Dec 6, 2023
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BIOS251 OL, Week 3 Lab
Name:
OL Lab 3: Cell Membrane and Transport: Learn how transporters keep cells
healthy
Learning Objectives:
•
Describe the plasma membrane structure using the fluid mosaic model
•
Recognize the relative permeability of lipid bilayers to different classes of
molecule
•
Compare active and passive transport of molecules
•
Identify the 3 modes of active transport and the different classes of ion channel
and carrier molecules
•
Relate the expression of specific transport proteins to the cell’s role
Introduction
:
A variety of substances move in and out of the cells through the plasma membrane.
Membrane transport is vital for cellular activities. The cells need to be healthy for the
body to work efficiently. From filtration in the kidneys to the contraction of muscles
during exercise, membrane transport contributes to many processes. In this simulation,
you will learn about the structure and function of the cell membrane, and discover why
membrane transporters are vital for healthy cells and the function of organ systems.
In the first part of your mission, you will be introduced to the concepts of selective
permeability and the fluid mosaic models of the plasma membrane. You will explore
why cells need specialized transporter proteins to transport cargo molecules across
their membranes. While some molecules can diffuse across the cell membrane, most
molecules require a transporter protein to enter or leave the cell.
In the later part of the simulation, you will set up a fluorescence microscopy experiment
to learn how the channels, carriers, and pumps that exist in the membrane ensures that
only the right molecules enter under the right conditions and help not only to keep the
cell healthy but also help organ systems to function.
Part 1: Complete Labster Cell Membrane and Transport
Part 2: Report and Reflection
BIOS251 OL, Week 3 Lab
Name:
Purpose: Describe in your own words and in complete sentences, the purpose of this
experiment.
The purpose is for us to get our self-familiarize with the concept and
understanding about how the fluid mosaic works.
Observations: List 2 observations you have made in this simulation.
The Observation is that hypertonic is when the cell shrink and hypotonic is when the cell
swell.
Answer all the questions below:
1. In complete sentences and in your own words define the following cellular
transport: (2 points)
a. Diffusion: is the movement of particle that moves down in the concentration
gradient which goes from high to low concentration.
b. Osmosis: Is the movement of water molecules moving down from high to low
concentration.
c.
Facilitated diffusion: Is when the solutes diffuse in the plasma membrane by
transport protein.
d. Active transport: Is when solute move from low to high concentration across the
concentration gradient.
BIOS251 OL, Week 3 Lab
Name:
2. Using the figure below, identify the cellular structures and its function. (6 points)
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Lecture 6-Worksheet
Part 1: Below are TWO ER bound proteins. The green region is indicating the ER transfer signals.
1. Can you label the start and stop transfer signals based on the rules we just went over?
2. Draw and label these proteins.
(1)
(2)
N
N
ER lumen
Part 2: Sketch the orientation of this protein as it would appear in the transport vesicle and plasma
membrane.
membrane-enclosed
organelle
(e.g., ER or Golgi)
vesicle
plasma
membrane
1₂:0.
0-9
?
CYTOSOL
extracellular fluid
165
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need help with Q6- the table above connect with helping answer the table.
Thanks!
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Unit 2: Cells Test Review
Cell Transport
Label the following pictures: active transport, passive osmosis, passive diffusion, or passive facilitated diffusion.
We o
Why does Active Transport require ATP energy?
What is your vocabulary word that means balance/ equillibrlum?
Solutions
1. Label the below images of solutions: hypertonic, hypOtonic, or isotonic.
2. Write if the cell will be at equilibrium, shrink, or swell.
3. Explain where the most water is (inside the cell or outside the cell).
4. Explain where most of the water will move (inside the cell or outside the cell).
Water O
Water
Water
Water o O
Water
Water
O Solute
O Solute
O Solute
Explain if water will fNow into or out of the cell in the following scenarios:
1. Salt is 30% inside the cell and 35% outside the cell.
• Water is
% inside the cell
% outside the cell
• Waler will flow [inside /outsidel so the cell can reach equilibrium.
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he cell
TOPIC 2.4 Plasma Membranes
ENDURING UNDERSTANDING ENE-2 Cells have membranes that allow them to establish and maintain internal environments that ere diferent
from their external environments.
LEARNING OBJECTIVE
ENE-2.A Desaribe the roles of each of the
components of the cell membrane in
maintaining the intemal environment of the cell.
Label the hydrophobic and hydrophilic
parts of one phospholipid.
There are three proteins embedded in the
membrane. Choose a color for
ESSENTIAL KNOWLEDGE
ENE-2.A.1 Phospholipids have both hydrophilic hydrophobic R groups
and hydrophobic regions. The hydrophilic
phosphate regions of the phospholipids are
oriented toward the aqueous external or
internal environments, while the hydrophobic
and hydrophilic R groups.
Color oode the regions of each protein.
Predict what would happen if a mutation
caused one of the hydrophobic R groups to
be switched with a hydrophilic R group.
Explain your thoughts:
fatty acid regions face each other within the…
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Uncommon
Schools Change Hstary
Name:
Date:
U2L3 -8th Science: Cell Transport: Diffusion & Osmosis
Team:
7.
What is the difference between osmosis and diffusion?
8. For each of the situations below, indicate the water concentrations both inside and outside
the cell.
1% sugar
3% sugar
1% sugar
5% sugar
1% sugar
1% sugar
9.
In each of the situations above, indicate the direction of water movement
10. Explain why you drew the arrows in that direction.
11. Where would water move?
5% NaCI
5% NaCl
95% H2O
95% H2O
10
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8. Define homeostasis.
maintoining nterral balance
9. What role does the cell membrane play in maintaining homeostasis?
10. How is facilitated diffusion different from diffusion? How are they similar?
11. List two ways that active transport is different than passive transport.
1)
2)
12. Why is the sodium-potassium pump considered an active transport? Which direction are the
sodium and potassium bing pumped? How many sodiums are being pumped? How many
potassiums are being pumped?
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ntity_Sound+of+Waves (1) x
S Forrar (wrap) y decorar tu libre
S Schoold
A districtims.seattleschools.org/common-assessment-delivery/start/5398502362?action3Donresume&submissionld%=657119921
Concentration, Osmosis, and Cell Environments HW Quiz
Assume that the picture below shows environments on two sides of a selectively permeable membrane. Assume the light blue circles are wa
molecules. Assume the red circles are glucose molecules. Which side has the higher concentration?
water molecules will flow to the left
water molecules will flow to the right
water molecules will flow both ways, but mostly will flow to the right
water molecules will flow both ways, but mostly will flow to the left
water will flow to the left and glucose will flow to the right
water will flow to the right and glucose will flow to the left
Which best describes the way that the particles move?
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Extension Questions:
Rate of Transport ->
Concentration difference
Active Transport
Diffusion
Facilitated Diffusion
39. Using your knowledge of mechanisms of transport across membranes, suggest why
the rate of diffusion shows a linear trend as concentration difference increases,
whereas the rate for facilitated diffusion eventually reaches a maximum.
40. Why does active transport, on the same graph, start off with such a high initial rate
compared to diffusion and facilitated diffusion?
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Part I – Introduction to Diffusion and Osmosis1. Define the terms diffusion, passive transport, active transport, and osmosis. In each of your definitions, describe the role of a concentration gradient. 2. Biological membranes are said to be selectively permeable (or semi-permeable). What does this term mean, and how does this affect the way that molecules are able to move through cellular membranes? 3. Which type of molecule is more likely to quickly pass through a cellular membrane via simple diffusion, polar or nonpolar? Why? (You may need to use information from your textbook and class discussions to answer this question.)
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4U
Goals
on Skills
Action
IIUW CU Small sites Italie Chanyes!
Choose one of the graphs below and interpret and analyse the graph about cellular transport.
Please comment on another classmate's post who chose to do a different graph from you.
Graph 1
300
450-
400-
facilitated diffusion
350-
Rate of glucose uptake /
300-
mmol cm
→cells hr-
250-
200-
150-
100-
50-
simple diffusion
0-
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
External concentration of glucose / mmol dm
The rate of glucose uptake by facilitated diffusion and simple diffusion in different concentrations of
glucose.
by Jay Reimer
Graph 2
AUG
active transport
2
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23
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A
Q
5. When given images such as the ones below, be able to identify the specific type of cell
transport shown. Practice using this online interactive activity. Passive or active
transport
B
Type of Transport
Page 8 of 10 761 words MTX English (United States)
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LAB EXERCISE 3A
A. Cell Membranes. In the square provided, draw a double layer of phospholipids with a large protein
embedded within it. Label the parts of this phospholipid bilayer that are partially charged (polar) and
uncharged (non-polar).
B. Anatomy of a Cell. Draw a "typical" cell in the space below (see lecture notes or the
labeled/unlabeled Images on Canvas). Sketch the following organelles within the cell and label them.
Plasma (Cell) Membrane
Nucleus
Ribosome
Rough Endoplasmic Reticulum
Smooth Endoplasmic Reticulum
Golgi Apparatus
Mitochondria
Lysosome
Cytoplasm
Microvilli
Cilia
A region of exocytosis
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What is isotonic solutions hypothetical concentration of solvent and solute?
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only do the letter d
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Pls help ASAP.
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15
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I need help
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11:36 PM Fri Oct 9
- 28% I
T
of
+: 0
List 4 functions of the cell or plasma membrane:
1.
2.
3.
4.
Correctly color code and identify the name for each part of the cell membrane.
Name/Color
Phospholipid bilayer (no color)
Name/Color
Cholesterol (green)
Letter
Letter
Transport protein (pink)
Carbohydrate (red)
Fatty acid tails (orange)
Phosphate heads (yellow)
王
F
B
b.
Match the cell membrane structure or its function with the correct letter from the cell membrane diagram.
Letter
Structure/Function
Letter
Structure/Function
Attracts water
Repels water
Helps maintain flexibility of
Make up the bilayer
membrane
Involved in cell-to-cell
Help transport certain materials
recognition
across the cell membrane
2
3
>
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please help
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decor
isLhetims.seattleschools.org/common-assessment-delivery/start/5398502362?action3Donresume&submissionld%=657119921
Concentration, Osmosis, and Cell Environments HW Quiz
For each of the drawings, label what kind of environment it is in, how you know this, and what is happening to the cell.
This cell is in a isotonic
v solution.
I know this because
This cell will
More water is coming in than going out
More water is going out than coming in
The same amount of water is going out and coming in
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Physiology - question 1 a-c
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Learning Goal:
A red blood cell is placed into each of the following solutions. Indicate whether crenation, hemolysis, or neither will occur.
To learn about osmosis and about isotonic solutions,
hypertonic solutions, and hypotonic solutions.
Osmosis is the net movement of water across a
semipermeable membrane from an area of lower
concentration to an area of higher concentration.
Solution A: 1.28 % (m^v) NaCl
Solution B: 2,46 % (m/v) glucose
Solution C: distilled H20
Solution D: 8.03 % (m/v) glucose
Solution E: 5.0% (m/v) glucose and 0.9% (m/v) NaCl
(Figure 1)
Drag each solution to the appropriate bin.
The water will continue to move across the
semipermeable membrane as the system attempts to
reach equilibrium, where both solutions have the same
concentration.
• View Available Hint(s)
Reset
Help
There are three different ways a solution can be
described in relation to a cell placed in the solution:
isotonic, hypotonic, and hypertonic. Compared to a
cell, an isotonic solution exerts the…
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Link each process with the correct definition. RECEPTORMEDIATED ENDOCYTOSIS PHAGOCYTOSIS PINOCYTOSIS EXOCYTOSIS 1. A cell ingests a large particle, such as a bacterial cell. 2. Receptor proteins embedded in the membrane recognize specific surface characteristics of substances. 3. A transport vesicle inside the cell approaches the plasma membrane of the cell, fuses with it, and releases its contents to the outside of the cell. 4. A vesicle containing whatever molecules are in solution outside the cell bulges inward, pinches off, and enters the cell.
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Please asap
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-Fill in the blanks.
-You must answer (1) to (27)
THANK YOU IN ADVANCE.
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Answer all pf the questions thanks
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- need urgent helparrow_forwardLecture 6-Worksheet Part 1: Below are TWO ER bound proteins. The green region is indicating the ER transfer signals. 1. Can you label the start and stop transfer signals based on the rules we just went over? 2. Draw and label these proteins. (1) (2) N N ER lumen Part 2: Sketch the orientation of this protein as it would appear in the transport vesicle and plasma membrane. membrane-enclosed organelle (e.g., ER or Golgi) vesicle plasma membrane 1₂:0. 0-9 ? CYTOSOL extracellular fluid 165arrow_forwardneed help with Q6- the table above connect with helping answer the table. Thanks!arrow_forward
- Unit 2: Cells Test Review Cell Transport Label the following pictures: active transport, passive osmosis, passive diffusion, or passive facilitated diffusion. We o Why does Active Transport require ATP energy? What is your vocabulary word that means balance/ equillibrlum? Solutions 1. Label the below images of solutions: hypertonic, hypOtonic, or isotonic. 2. Write if the cell will be at equilibrium, shrink, or swell. 3. Explain where the most water is (inside the cell or outside the cell). 4. Explain where most of the water will move (inside the cell or outside the cell). Water O Water Water Water o O Water Water O Solute O Solute O Solute Explain if water will fNow into or out of the cell in the following scenarios: 1. Salt is 30% inside the cell and 35% outside the cell. • Water is % inside the cell % outside the cell • Waler will flow [inside /outsidel so the cell can reach equilibrium.arrow_forwardhe cell TOPIC 2.4 Plasma Membranes ENDURING UNDERSTANDING ENE-2 Cells have membranes that allow them to establish and maintain internal environments that ere diferent from their external environments. LEARNING OBJECTIVE ENE-2.A Desaribe the roles of each of the components of the cell membrane in maintaining the intemal environment of the cell. Label the hydrophobic and hydrophilic parts of one phospholipid. There are three proteins embedded in the membrane. Choose a color for ESSENTIAL KNOWLEDGE ENE-2.A.1 Phospholipids have both hydrophilic hydrophobic R groups and hydrophobic regions. The hydrophilic phosphate regions of the phospholipids are oriented toward the aqueous external or internal environments, while the hydrophobic and hydrophilic R groups. Color oode the regions of each protein. Predict what would happen if a mutation caused one of the hydrophobic R groups to be switched with a hydrophilic R group. Explain your thoughts: fatty acid regions face each other within the…arrow_forwardUncommon Schools Change Hstary Name: Date: U2L3 -8th Science: Cell Transport: Diffusion & Osmosis Team: 7. What is the difference between osmosis and diffusion? 8. For each of the situations below, indicate the water concentrations both inside and outside the cell. 1% sugar 3% sugar 1% sugar 5% sugar 1% sugar 1% sugar 9. In each of the situations above, indicate the direction of water movement 10. Explain why you drew the arrows in that direction. 11. Where would water move? 5% NaCI 5% NaCl 95% H2O 95% H2O 10arrow_forward
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