Bacterial Genetics Flashcards

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San Jacinto Community College *

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3304

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Biology

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Dec 6, 2023

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11/26/23, 5:48 PM Bacterial Genetics Flashcards | Quizlet https://quizlet.com/238046570/bacterial-genetics-ﬂash-cards/ 1/5 Try the fastest way to create flashcards Bacterial Genetics 39 studiers today 4.2 (6 reviews) Students also viewed Terms in this set (52) Try Magic Notes and save time Instantly generate flashcards, practice tests and more LAB FINAL: part 1 (10) 76 terms caseypurvis Preview Microbiology Lab: Bacterial Genetic ... 61 terms wnn4 Preview MICRO - Bacterial Genetics 24 terms mtkolt34 Preview LAB EXAM 1 56 terms CadeeCa all RNA molecules serve as templates for protein synthesis false indicate the four nitrogenous bases that are found in DNA adenine, guanine, cytosine, thymine the bonds that join the nucleotides in DNA to form the sugar-phosphate backbone are ___ bonds covalent a single nucleotide change in a DNA sequence is not enough to alter any protein structure in an organism false match the analytic method with the description * DNA fingerprinting/profiling = demonstrates unique patterns of DNA fragments in a gel. * polymerase chain reaction(PCR) = amplifies a specific DNA sequence so that there are enough coples that they can be readily detected if present. * DNA sequencing = determines the order of nitrogenous bases in DNA. * DNA probes = detects specific short DNA sequences characteristic of a specific microorganism the process of copying a strand of DNA to form a strand of RNA is called transcription

11/26/23, 5:48 PM Bacterial Genetics Flashcards | Quizlet https://quizlet.com/238046570/bacterial-genetics-ﬂash-cards/ 2/5 all mutations result in phenotypic change in an organism false the deliberate manipulation of an organism's genome in the laboratory is specifically known as ___ genetic engineering which of the following best portrays the direction of genetic information flow, according to the central dogma of biology DNA -> RNA -> Protein select the three components of a nucleotide in DNA deoxyribose, nitrogenous base, phosphate in which of the following ways does the gene for sickle cell hemoglobin differ from the gene for normal hemoglobin there is a single nucleotide difference different species of microorganisms as well as different strains within a species can usually be distinguished by genetic differences true a single nucleotide change in the original sequence of DNA is a ___ mutation which of the following are potential applications of PCR detection of DNA during crime scene analysis. detection and identification of infectious agents in patient samples. detection of DNA from victims of massive disasters. match each of the items needed for PCR with their function in the reaction * template DNA strand = provides the target sequence which will be amplified * primers = direct the DNA polymerase to the target sequence and provide starting material for DNA polymerase to add on to * thermostable polymerase = synthesize new DNA strands * thermocycler = controls and quickly modifies the reaction temperature as needed * free deoxynucleotides = building block that are added to form the new DNA strands place the steps of one PCR cycle into the correct order 1. denaturation 2. priming 3. extension primers selected for PCR must hybridize with ___ the DNA flanking either end of the target sequence following PCR, the products were loaded into a gel and electrophoresis was performed. following staining of the gel, no band of DNA was observed. based on this observation, was the desired sequence successfully amplified no which of the following is accomplished by use of PCR a specific sequence of DNA can be amplified a billion fold

11/26/23, 5:48 PM Bacterial Genetics Flashcards | Quizlet https://quizlet.com/238046570/bacterial-genetics-ﬂash-cards/ 3/5 indicate the items needed for PCR free deoxynucleotides thermostable DNA polymerase primers template DNA with target sequence primers provide the specific location where DNA synthesis will ___ begin which of the following can be used to detect the products of PCR gel electrophoresis match the reagents used in the master mix with their purpose in the polymerase chain reaction taq polymerase = heat-stable enzyme that builds the new DNA molecules dNTP mixture = free nucleotides used to build the new DNA molecules primers = short DNA sequences that direct the synthesis of the target DNA buffer = a reagent used to enhance the activity of enzymes in the mixture the following steps are completed when preparing a sample for PCR. place the steps in the correct order 1. label microcentrifuge tubes 2. add DNA samples to microcentrifuge tubes using a new micropipet tip for each sample 3. add prepared master mix containing Taq polymerase, primers, deoxynucleotides, and buffer to microcentrifuge tubes 4. place PCR tubes into thermocycler, set program, and start how should PCR products be stored after completion of PCR, prior to gel electrophoresis 4C which of the following are needed to cast the agarose gel comb, liquefied agarose, gel tray when positioning the gel in the electrophoresis chamber, the wells should be closet to the ___ electrode negative the progress of the DNA fragments through the gel can be monitored by ___ observing the movement of the bromophenol blue loading dye where should the micropipet tip be when delivering a DNA specimen into a well in an agarose gel below the buffer surface, in the upper portion of the well what would be the consequence of not including a lane with the DNA ladder on an electrophoresis gel there would be no way to determine the size of the fragments which of the following is used to stain the DNA in a gel for viewing after electrophoresis ethidium bromide which of the following are included in the master mix used to perform PCR deoxynucleotides primers buffer taq polymerase

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