Homework 6- transformation
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Homework 6- Due April 1
st
Activity 1:
Watch the following video and answer the questions below:
https://www.labxchange.org/library/items/lb:LabXchange:255eb5d5:video:1
Please watch the above Transformation video
, and answer the following questions:
1.
What is the purpose of adding calcium chloride while preparing competent cells? (
1
pt.
) 2.
If heat shock is needed to open the cells so the plasmids can enter, why do we place the cells on ice for a few minutes after heat shock? (1
pt
.) 3.
Why do we label the LB plates on the bottom side? (1
pt
.) 4.
Why do you add 500 ul of SOC medium to the cells after transformation? (1
pt
.) Activity 2:
Please, read the Plating Bacteria and Selective Media
article and answer the following questions: https://www.labxchange.org/library/items/lb:LabXchange:40c53f02:html:1
1.1 Imagine you transformed competent E.coli
cells with pUC19 plasmid (in P+ tube) and set up a negative control transformation, where competent E.coli cells were mixed with
water instead of plasmid (in P- tube). You plated the cells from both tubes on two types
of media: LB and LB/Amp. Would you expect the formation of bacterial colonies on these plates for each transformation (Yes/No)? (
4 pt
.)
Media/Plate
Tube/Transformation
P+
P-
LB
LB/Amp
2.2. If you transformed bacteria with a plasmid carrying the kanamycin (Kan) resistance gene and a gene for green fluorescent protein (GFP) under the control of an arabinose-
dependent promoter, what media can you use to observe the bacteria grow? What media can you use to observe the bacteria glow? (Yes/No) (2 pts.)
Transformed
bacteria are able to:
LB
LB/Amp
LB/Kan
LB/Kan/ara
Grow?
Glow?
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H Take Test: Lab Quiz 3-2020 Fall X
G You look under a microscope at
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A https://bbhosted.cuny.edu/webapps/assessment/take/launch.jsp?course assessment id3_1640978_1&course_id%3_190070m
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* Question Completion Status:
3
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QUESTION 5
Which type of epithelia would make up an organ that needed to be able to expand to many times it's normal size?
O Pseudostratified columnar
O Simple cuboidal
O Transitional
O Simple squamous
QUESTION 6
You look under a microscope at 400X magnification and see an empty space lined with multiple layers of cells with flattened nuclei, and a large amount
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ension to alequiad
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