10+-+Micro+Lab+-+Endospore+Stain_Fillable

A student needed to transfer bacteria from a broth culture to an agar plate. Below is the step-by-step what was done to accomplish this. The transfer of bacteria was not successful because of which step? 1. Cap of the broth culture is removed 2. The mouth of the bottle is flamed 3. The loop was flamed 4. The loop was inserted into the culture to pick up the bacteria 5. The loop was flamed 6. The loop containing the bacteria was used to introduced to spread the agar plate 7. The plate was placed in an incubator at 30 Celcius

Select all that apply to a direct simple stain:    1. the dye is acidic or negatively charged and stains the background 2. uses multiple dyes in the procedure 3. cells will never be distorted or shrunken 4. uses only 1 dye in the procedure 5. does involve a washing step 6. can be used to determine cell morphology, size, and arrangement. 7. does involve fixing 8. the dye is basic or positively charged and stains the bacteria 9. cells may be distorted or shrunken 10. does not involve a washing step 11. does not involve fixing

Select all that apply to a negative stain: uses only 1 dye in the procedure O uses multiple dyes in the procedure involves fixing does not involve fixing O involves a washing step does not involve a washing step uses a basic or positively charged dye which stains the bacterial cells uses an acidic or negatively charged dye which stains the background O can show cell morphology, size, and arrangement Microsoft Bing 11:33 AM 87°F Sunny 9/14/2021

Out of twelve tests that can be performed:  Gram Stain Wet Mount Endospore Staining Acid Fast Staining Differential & Selective Media (MacConkey, PEA, EMB, MSA Agar, Blood Agar, etc.) Oxidative-Fermentative Test Carbohydrate Fermentation MR-VP Testing Indole Production Citrate Utilization Triple Sugar Iron Testing Catalase Test.  Which four tests are the best fit to help identify bacteria that is unknown? Possible bacterias that are being observed are:    Enterobacter aerogenes Escherichia coli Micrococcus luteus Proteus vulgaris Pseudomonas aeruginosa Salmonella typhimurium Serratia marcescens Staphylococcus aureus Staphylococcus epidermidis

Select all that apply to a negative stain:    1. involves a washing step 2. cells may be distorted or shrunken  3. uses an acidic or negatively charged dye which stains the background 4. uses multiple dyes in the procedure 5. uses only 1 dye in the procedure 6. involves fixing 7. does not involve fixing 8. cells will not be distorted or shrunken 9. does not involve a washing step 10. can show cell morphology, size, and arrangement 11. uses a basic or positively charged dye which stains the bacterial cells

Which stain can be used to determine if bacteria are capsulated?    Question options:   flagella stain   negative stain   simple stain   capsule stain

INSTRUCTION: Answer the question properly Do not copy in Google, plagiarize checker will be used. QUESTION: Discuss the various theories on the Gram stain.

After performing a Gram stain on a Gram negative bacteria, the specimen does not show pink or purple bacteria. Which of the following could notexplain why this occurred?   Decolorizer was used after safranin     Safranin was not used   Crystal violet was not used     The specimen was not heat-fixed

Which of the following statements is FALSE?   Question options:   stains increase the contrast of a specimen with its surroundings   all of these statements are TRUE   dyes generally consist of a chromophore and an ion   the chromophore of an acidic dye is positively-charged   simple staining procedures use a single dye   basic dyes bind to negatively-charged molecules

Before viewing a specimen of pigmented bacteria on a slide under the light microscope, which of the following usually needs to happen (and why)?     Staining (to increase magnification).     Heat, radiation or antimicrobial chemical treatment (to kill the bacteria for safe observation).     Add immersion oil (to increase resolution).     Add a cover slip (to reduce contamination).     The viewing chamber needs to be flushed of air (to create a vacuum).

Complete

Table 1. Different Staining Techniques Gram Stain Acid Fast Endospore Capsule Flagella 1. Principle 2. Characteristic of Bacteria viewed using this stain. (e.g. bacterial type, strain, or targeting particular bacterial feature/s) 3. Stain/s Used 4. Type of Stain 5. Mechanism of Staining stain used) (of each 6. Flow of Method

name 5 things about method staining.

Choose the 4 most correct steps from the list below for a simple positive stain and organize them into the most correct order. Briefly explain the purpose of each step you chose. • Heat fix • Dip slide in acid alcohol • Wash slide with ethanol • Apply Congo Red to the slide • Apply Crystal Violet to the slide • Make a smear • Wash slide with water

Sample Station 7 A. If a student performs a Gram stain on a Gram negative bacterium and stops the stain technique after the iodine step and rinse, what color would you predict the cells to be if you looked at it right now under the microscope?

DESCRIBE THE BACTERIA OBSERVED. DO NOT DEFINE THE STAINING TECHNIQUES. PLEASE REFER TO THE PHOTO.

Indicate color staining results of gram positive and negative bacteria for every hypothetical scenario.   Modification in Procedure Staining results 1.   Crystal violet was replaced with methylene blue. Gram positive Gram negative 2.   Mordant was skipped Gram positive Gram negative 3.   Decolorizing step was omitted Gram positive Gram negative 4.   counterstain was not used? Gram positive Gram negative 5.   Crystal violet and safranin was swapped in position. Gram positive Gram negative

What is the purpose of fixing a smear? Mark all that apply: 1. To attach the bacteria to the slide  2. To cause the cells to shrink and become distorted 3. To kill the bacteria so they aren't harmed by the staining method 4. To break down the cell wall in order to make the cells accept stain 5. To kill the bacteria to make the slide safer to handle

MICROBIOLOGY:  Microscopic Morphology of Microbes Write your introduction (This includes principles, significance of the study, objectives of the experiment and how the objectives were achieved. This part must also be in the passive voice and past tense. Introduction must be short but packed with relevant content). another: What is the advantage of the Gram stain over the simple stain? What is the theory about the mechanism of the Gram-stain reaction?

Which of the following is/are true regarding the acid-fast stain? (There may be more than one correct answer.) Non-acid-fast microbes appear blue in a completed acid-fast stain. O Acid-fast cells retain the primary dye after treatment with acid-alcohol. O It is used to identify members of the genus Mycobacterium. V Acid-fast cells appear bright pink/red in a completed acid-fast stain. O If cells are acid-fast, they are gram-negative.

Submit screenshots of the Gram stain (1000x total magnification) and Endospore stain (1000x total magnification).

Out of the three gram-positive bacteria (Micrococcus luteus, Staphylococcus aureus, and Staphylococcus epidermidis).  Which of the following test(s) will help me identify the unknown gram-positive bacteria?     Gram Stain Wet Mount (motility testing) Endospore Staining Acid Fast Staining Differential & Selective Media (MacConkey, PEA, EMB, MSA Agar, Blood Agar, etc.) Oxidative-Fermentative Test Carbohydrate Fermentation MR-VP Testing Indole Production Citrate Utilization Triple Sugar Iron Testing Catalase Test

By the end of this section, you will be able to: • Differentiate between simple and differential stains • Describe the unique features of commonly used stains Explain the procedures and name clinical applications for Gram, endospore, acid-fast, negative capsule, and flagella staining

How does smear preparation of cells from a liquid medium differ from preparation of cells from a solid medium? Mark all that apply:     A smear made from liquid media will be thinner than a smear made from solid media A smear made from solid media doesn't have to be fixed but a smear from liquid media does A smear made from liquid media will be more thick than a smear made from solid Water must be added to a smear made from solid media but not liquid media A smear made from liquid media doesn't have to be fixed but a smear from solid media does Water must be added to a smear from liquid media but not from solid

Please provide the principle of gram staining method.

The first step in identifying an unknown bacterium is to __________. The first step in identifying an unknown bacterium is to __________. culture your unknown on selective media perform endospore staining on your culture prepare a pure working stock simple staining on your culture acid-fast staining on your culture

Worksheet Table 1. Special Staining Methods Staining Technique Stain Cellular structure/ Bacteria Example : Albert Malachite green and Metachromatic granules toluidine blue Dorner Dyar Feulgen Fontana-Tribondeau Gray Leifson Levaditi's Neisser Nigrosin Schaeffer-Fulton

hello, can you draw the following on endospore staining in 1000x objective? thank you.   - 1 day old bacillus - 5 day old bacillus

Can you help me?

Subculture and Colony Morphology Descriptions Following identification of the Gram-negative isolate and Gram-positive isolate, you next subculture each onto fresh nutrient agar plates.  Briefly describe the subculture process in three or so sentences and what this allowed you to achieve; follow this with colony morphology descriptions of each.    Description:           Isolate A – Describe:    Colony morphology:    Medium & incubation temperature: Isolate B – Describe:    Colony morphology:    Medium & incubation temperature:

Complete for Additional Differential Staining B: Negative staining ~ thin smear C: Endospore Stain

Explain the relationship between bacterial cell wall with gram-positive and gram-negative in the staining process. Please state chemical used and their function.

For a laboratory exercise of basic techniques in microbiology: Gram stain and Microscopy. What are the sources of error in performing Gram staining?