DATA 07 - FALL 2021 (1)

D Question 37 Based on the data below, how much more UV resistant was B. licheniformis relative to S. aureus? (All controls had growth.) - covered with lid Organisms Exposure 20 sec Staphylococcus aureus 5 sec + 20 sec Bacillus licheniformis + 10 sec 80 sec times to UV light 40 sec 2.5 min 160sec 5 min 10 min 5 min 20 min 10 min 40 min 10 min + 40 min

Sulfur Indole Motility (SIM) Medium H2S produced, color and +/-: ______________________________ Indole present/Tryptophan hydrolysis, color and +/-: ___________________________ Motile or non-motile: _____________________________

Answer the questions. Cite your reference. You received three swabs from a wound discharge, and you placed them on enrichment media. You noticed growth was distributed throughout all the media; there was abundant growth toward the surface of the medium in one tube, whereas other tubes showed an equal distribution of growth throughout the tubes. Explain why this happened.

procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (1) broth culture to broth

Hello, please read the attached Microbiology question and complete the entire chart given correctly. *If you correctly complete the entire chart with the correct answers, I will provide a Thumbs Up for you Thank you.

Direction: Read and analyze the following laboratory experiment and answer the following question. PART 1: SURFACE AREA AND CELL SIZE Materials: Agar containing NaOH, and the pH-indicator dye phenolphthalein cured into cubes of various size, 3 plastic cups, HCl, metric ruler, paper towels. Methodology: 1. Safety: Wear goggles and nitrile gloves while completing this lab. 2. Obtain three different size blocks of pink or blue agar. Using a ruler, measure the length, width, and height of the three blocks given below. Cut the agar according to the given dimension. Small = 1 cm x 1 cm x 1 cm Medium = 2 cm x 2 cm x 2 cm • • Large = 1 cm x 1 cm x 6 cm 3. Record your data. 4. Pour HCl or vinegar into two small cups. Place the one larger "cell" into one cup and the two smaller cells in the other cup. Start timing 30 minutes. 5. After 30 minutes, remove the cells and blot them dry with a paper towel. 6. Using your ruler, measure the distance the HCl has diffused into the blocks as shown on the…

Topic: yeast cells what is the purpose of cover slip in Dalmau plate? please elaborate

1:07 < Вack Untitled 6 Brownian movement of bacteria: O a. Can be observed by the of soft agar technique O b. Can be observed by staining of Staphylococcus aureus Oc. Is common in Escherichia coli or Proteus vulgaris O d. Is common in Staphylococcus aureus O e. Can be observed at the edge of a microscope CLEAR MY CHOICE

Area of no growth around an antibiotic disk indicates_________________.

Case Study: You are asked to inoculate lactose media (broth) with E. coli that was growing exponentially in glucose media (broth). You monitor the growth of these cells in the lactose broth using a viable plate count technique and plot the Growth Curve for E. coli growing on lactose. After a day of monitoring the cells, you note that the culture entered into lag phase growth initially and is now currently growing exponentially. In addition, you find that the cultures doubling time is 30 minutes. You know that the doubling time of E. coli in glucose media is 20 minutes. You did not change the oxygen content or temperature at which you were growing the cells, E. coli is still aerobically respiring at 37°C. Question: In order to determine the doubling time of E. coli a viable plate countris performed. This means that you are truly counting O Dead cells only via a microscopic count O Live cells only via membrane filtration or serial dilution O All cells via membrane filtration or serial…

Case Study: You are asked to inoculate lactose media (broth) with E. coli that was growing exponentially in glucose media (broth). You monitor the growth of these cells in the lactose broth using a viable plate count technique and plot the Growth Curve for E. coli growing on lactose. After a day of monitoring the cells, you note that the culture entered into lag phase growth initially and is now currently growing exponentially. In addition, you find that the cultures doubling time is 30 minutes. You know that the doubling time of E. coli in glucose media is 20 minutes. You did not change the oxygen content or temperature at which you were growing the cells, E. coli is still aerobically respiring at 37°C. Question: If you want to grow E. coli as quick as possible would you grow it on lactose or glucose? O Mixing the two carbon sources would provide the most optimal growth conditions. O Lactose because of the generation time being faster. O Lactose and an anaerobic environment. O Glucose…

Case Study: You are asked to inoculate lactose media (broth) with E. coli that was growing exponentially in glucose media (broth). You monitor the growth of these cells in the lactose broth using a viable plate count technique and plot the Growth Curve for E. coli growing c lactose. After a day of monitoring the cells, you note that the culture entered into lag phase growth initially and is now currently growing exponentially. In addition, you find that the cultures doubling time is 30 minutes. You know that the doubling time of E. coli in glucose media is 20 minutes. You did not change the oxygen content or temperature at Which you were growing the cells, E. coli is still aerobically respiring at 37°C. Question: If the lac operon is 'on' what does this imply about the cell? O The cell is dying and the lac operon is going to induce apoptosis. Apoptosis is truly cell death and the cells will enter into the death phase of the Growth Curve. O Lactose is being catabolized and glucose is no…

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Factors which can influence antibiotic susceptibility testing (size of zone inhibition) are_________________.

What does the phrase "pipetting up and down" mean and why is this technique used? The phrase "pipetting up & down" means to triturate. Th technique is used to mix (or homogenize) a solutic 7 On what part of a microcentrifuge tube should you write a label? Describe the order in which you filled the tubes in Step 2 of Procedure 4. Did this order result in maximum efficiency? If not, what order would be most efficient? 9 What does the phrase "spinning down" mean and why is this technique used? The word 'spinning down" means, To diminish in energy i to slow down o out; to be gradually ended or concelled. It is used to reduce its spin speed from that required for reading and writing. 10 Why was the comb placed in the middle rather than at one end of the gel for this electrophoresis experime- The comb is in the center of the gel Since the dyes used have positive or negative charges and can therefore migrate in differen

Please you the first image as an example to fill the gaps on the 2nd image .please no explanation, just fill in the gap . Thanks

Test Description Terminal Terminal Medium, Incubation Incubation Results temperature time (°C) electron enzyme reagent ассeptor 1 Oxidase test Tryptic 24 – 48 hours soy agar (TSA), QxiDrop 2 Catalase Охудen test N/A Nitrate 37 reductase N/A test

S. epidermidis ~1 micron • Note: just focus on the round shape bacteria only (The pink color should be ignored for this lab)

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General Instructions:Choose 1 bacteria/diseases and fill out the details. An example of an answered template is also provided for your reference.    Causative Agent and Disease Profile for S. aureus Template and Example   ITEM MSM PROFILE   MICROBIAL PROFILE   I MICROORGANISM/CAUSATIVE AGENT Staphylococcus aureus A GRAM REACTION (+) B OXYGEN REQUIREMENT Facultative Aerobes C SIZE 1.5 µm D SHAPE Cocci in clusters E HABITAT Normal flora of skin/anterior nares/pharynx F DISCOVERY   G MICROSCOPIC IMAGE   II DISEASE PROFILE Scalded skin syndrome A DISEASE/S                        Skin and Wound Infections       Scalded Skin Syndrome Toxic Shock Syndrome Food Poisoning Pneumonia B SYMPTOMS OF THE DISEASE A high fever · Nausea and vomiting · A rash on your palms and soles that resembles a sunburn C INCUBATION PERIOD 2 and 4 hours (range 30 minutes to 8 hours) D MODE OF…

Hello, please read the attached Microbiology question and complete the entire chart given correctly. *If you correctly complete the entire chart with the correct answers, I will provide a Thumbs Up for you Thank you.

Confluence cells added onto 100-wells plate, 2 ml trypsin added onto cells and 6 ml media added to dilute trypsin, 1 ml was used for counting, and average number of Cells counted was 24(DF 2) find out the the number of cells/ml and total number of cells in 7 ml? How many cells you need for 100 wells, assuming Number of cells/well is 10 to the power 4 cells?

please answer the following question

The figure above depicts an agar cube with a side length of 13\, \text{mm}13mm13, start text, m, m, end text. In an experiment, students submerged the cube in red dye for 121212 hours. The red dye permeated 1\, \text{mm}1mm1, start text, m, m, end text on each side, as indicated by the shading in the figure. Volume of a rectangular solid: V = lwhV=lwhV, equals, l, w, h Calculate the volume of the agar cube that remained unpenetrated by the red dye.

ANSWER THE FOLLOWING QUESTIONS REGARDING GEL ELECTROPHORESIS 1.why is it not advisable to move/touch the agarose gel in the process of hardening 2.what is the use or function of the TAE buffer that is poured or found in the gel box 3.how can you tell if agel is running 4.outline the process use in the preparation of agarose gel of 1.5 concentration 5.outline the processes/steps used in gel electrophoresis 6.name twoexamples of the dye used in gel electrophoresis 7.how do we prepare x1(concentration) TAE BUFFER form 50x TAE buffer

ISOLATE #1 Results for "Isolate #1" ***Please fill out the table**** 1. Blood agar: 2. Nitrate Test: Test Results Interpretation Blood agar (type of hemolysis) Nitrate test 3. Gelatinase Activity: Gelatinase activity Resistant or Susceptible? Novobiocin Resistance Zone Diameter: 4. Novobiocin Resistance: 5. Coagulase Test: Coagulase (Sure-vue test) 1. Based on your results, what is the possible identification (Genus and species) of “Isolate #1"? I 9 10 11 12 3 14

Answer the following questions:   1. Define a bacterial colony.   2. What is the difference between macroscopic and microscopic appearance of bacteria?   3. State the three standard terms used to describe single colonies on agar plates.   4. State and define the three types of growth that may be seen in a broth culture.   5. State three basic shapes of bacteria.   6. State and describe the different arrangements of cocci.   7. What is the difference between true motility and Brownian motion?

Hello, please read the attached Microbiology question and answer the question correctly. *If you correctly answer the question, I will provide a Thumbs Up to you. Thank you.

A 1.5-year-old child developed vomiting, diarrhea, and fever. Stool sample were inoculated into the Endo media. After 18 hours on the surface of the medium grew medium-sized, round, slightly convex red colonies with a metallic luster. The doctor suspected Escherichia coli. 1. Name the composition of the Endo agar media. 2. Describe the properties of bacterial colonies on the Endo media.  3. What purpose differential diagnostic Endo media used for?