Week 5 -- Selective and Differential Media Lab Assignment
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Apr 3, 2024
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Week 5 – Differential and Selective Media Lab Report
Name: ______________________________________________________
Phenylethyl Alcohol Agar (PEA) –
Looking at the results presentation (slide #1) fill in the data table below:
Bacterial species
Growth Y or N
Gram-positive or Gram-negative
Staphylococcus epidermidis
Escherichia coli
A.
What is the purpose of selective media?
B.
What is the selective agent in PEA media? How does it work?
Mannitol Salt Agar (MSA) – Looking at the selective and differential media results presentation (slide #2) fill in the data table below:
Bacterial species
Growth Y or N
Halophile Y or N
Color of Medium
Mannitol fermenter Y or N
E. coli
S. epidermidis
S. aureus
A.
What is the selective agent found in MSA?
B.
What is the differential agent found in MSA?
C.
What is the purpose of including the pH indicator phenol red in the medium?
Eosin-Methylene Blue Agar (EMB) – Looking at the selective and differential media results presentation (slide #3) fill in the data table below: Bacterial species
Growth Y or N
Color of Medium
Lactose Fermenter Y or N
Rapid Lactose
Fermenter Y or N
S. epidermidis
E. coli
E. aerogenes
S. marcescens
A.
What are the selective agents found in EMB? Which bacteria are able to grow on EMB?
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