Week 5 -- Selective and Differential Media Lab Assignment

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University of North Georgia, Dahlonega *

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2117

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Biology

Date

Apr 3, 2024

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docx

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4

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Week 5 – Differential and Selective Media Lab Report Name: ______________________________________________________ Phenylethyl Alcohol Agar (PEA) – Looking at the results presentation (slide #1) fill in the data table below: Bacterial species Growth Y or N Gram-positive or Gram-negative Staphylococcus epidermidis Escherichia coli A. What is the purpose of selective media? B. What is the selective agent in PEA media? How does it work? Mannitol Salt Agar (MSA) – Looking at the selective and differential media results presentation (slide #2) fill in the data table below: Bacterial species Growth Y or N Halophile Y or N Color of Medium Mannitol fermenter Y or N E. coli S. epidermidis S. aureus A. What is the selective agent found in MSA?
B. What is the differential agent found in MSA? C. What is the purpose of including the pH indicator phenol red in the medium? Eosin-Methylene Blue Agar (EMB) – Looking at the selective and differential media results presentation (slide #3) fill in the data table below: Bacterial species Growth Y or N Color of Medium Lactose Fermenter Y or N Rapid Lactose Fermenter Y or N S. epidermidis E. coli E. aerogenes S. marcescens A. What are the selective agents found in EMB? Which bacteria are able to grow on EMB?
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