mbio1220-practice-exams-with-answers

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MBIO1220 Practice exams with answers Essentials Of Microbiology (University of Manitoba) Scan to open on Studocu Studocu is not sponsored or endorsed by any college or university MBIO1220 Practice exams with answers Essentials Of Microbiology (University of Manitoba) Scan to open on Studocu Studocu is not sponsored or endorsed by any college or university Downloaded by Manjot Sawhney (manjotsawhney21@gmail.com) lOMoARcPSD|37627750

THE UNIVERSITY OF MANITOBA MBIO 1220 PRCACTICE Final Examination _____________________________________________________________________________________ __ READ THE FOLLOWING INSTRUCTIONS CAREFULLY:  The test consists of 100 multiple choice questions.  For each question, choose the response that best answers the question.  Name and student number must appear on both test paper and answer sheet.  Both the test paper and answer sheet MUST be returned. 1. Which of the following best describes the theory of “biogenesis”? a. Some bacteria can sour beer and wine – fermentation b. Some forms of life can spontaneously arise from non-living matter – spontaneous generation c. All living things are composed of cells – cell theory d. Living cells can arise only from pre-existing living cells 2. Which of the following statements is true of proteins? a. Proteins are made from long chains of amino acids held together by peptide bonds b. All proteins contain the elements carbon, hydrogen, oxygen and nitrogen c. Some proteins excreted by bacteria can be toxic to humans – exotoxins, endotoxin, cytotoxins, neurotoxins d. Some proteins produced by humans can neutralize viruses – interferons play an important role in immune protection towards viruses (chapter 14) . e. All of the above 3. A codon consists of: a. Three amino acids b. Three nucleotides c. Three DNAs d. Three proteins e. All of the above 4. The most important function of the bacterial cell wall is to: a. Resist antimicrobial agents – plasmid b. Allow the cell to attach to surfaces – fimbriae c. Protect the cell from osmotic lysis d. Trap alcohol during the Gram stain – gram positive cells e. Serve as a semi-permeable barrier – cytoplasmic membrane 5. Which of the following structures can NOT be found in a bacterial cell? a. Ribosomes b. Cytoplasm c. Mitochondria – bacteria do not have membrane bound organelles (nucleus, mitochondria and chloroplasts) d. Nucleoid Downloaded by Manjot Sawhney (manjotsawhney21@gmail.com) lOMoARcPSD|37627750

e. None, all of the above can be found inside a bacterial cell 6. What type of microscope would be best suited for observation of a bacterial inclusion body? a. Compound light microscope – bacteria, blood cell or hair but not DNA and nucleus b. Scanning electron microscope – surfaces c. Transmission electron microscope – internal structures (cytoplasm, nucleoid ,ribosomes, storage granules, endospores and sporulation) d. Scanning tunnelling microscope – see molecules depressions and bumps e. Telescope 7. True or False: The acid fast stain is a microscopy procedure that can be used to detect the lipid rich cell wall of bacteria waxy compound in cell wall from the genus Mycoplasma , mycobacterium the causative agent of walking pneumonia. a. True b. False Downloaded by Manjot Sawhney (manjotsawhney21@gmail.com) lOMoARcPSD|37627750

8. Which of the following statements about prokaryotic and eukaryotic microorganisms is true? a. Prokaryotic microorganisms can cause disease, while eukaryotic microorganisms cannot b. Prokaryotic microorganisms can be viruses, while eukaryotic microorganisms cannot c. Prokaryotic microorganisms can make peptidoglycan, while eukaryotic microorganisms cannot d. Prokaryotic microorganisms have sterols, while eukaryotic microorganisms do not e. Prokaryotic microorganisms can be chemoheterotrophs, while eukaryotic microorganisms cannot 9. Which of the following statements is true of Gram negative cell walls but not of Gram positive cell walls? a. They contain peptidoglycan b. They protect the cell against osmotic lysis c. They have teichoic acids d. They have an outer membrane containing lipopolysaccharide – gram positive doesn’t have an outer membrane instead it has teichoic acids which is wall teichoic and lipoteichoic acids/ gram positive has only one cell wall (cytoplasmic membrane) e. None of the above, all are true of both Gram negative and Gram positive cell walls 10. Fermentation does not use the following metabolic pathways: a. Glycolysis and the TCA cycle b. Glycolysis and the Electron transport chain c. The TCA cycle and the Electron transport chain – only in glycolysis d. Glycolysis, the TCA cycle and the Electron transport chain 11. The systematic oxidation of glucose to carbon dioxide and water occurs in the following order (from left to right): a. TCA cycle  Glycolysis  Electron transport chain b. Glycolysis  TCA cycle  Electron transport chain – basic knowledge c. Glycolysis  Electron transport chain  TCA cycle d. TCA cycle  Electron transport chain  Glycolysis e. Electron transport chain  TCA cycle  Glycolysis 12. The use of amino acids by an organism to build new proteins would be an example of: a. Catabolism – breakdown of complex organic molecules to simpler ones b. Glycolysis c. Anabolism - simpler ones to complex organic molecules d. Hydration 13. How do enzymes increase the rate of chemical reactions? – to lower the rate of chemical energy: increase temperature, pressure and enzymes decreases the activation energy which increases the rate of chemical reaction. a. They increase the activation energy of a reaction b. They decrease the activation energy of a reaction Downloaded by Manjot Sawhney (manjotsawhney21@gmail.com) lOMoARcPSD|37627750

c. They increase the temperature of a reaction d. They increase the pressure of a reaction e. None of the above 14. An organism that can grow in the absence of oxygen, but grows better when oxygen is present is known as a(n): a. Obligate aerobe b. Facultative anaerobe c. Obligate anaerobe d. Microaerophile e. Aerotolerant anaerobe 15. True or False: Okazaki fragments are formed on the lagging strand of DNA during chromosome replication. a. True b. False 16. You are working in an emergency ward, when you notice drops of blood on the floor. Which of the following chemicals would be best used to disinfect the floor? a. Ethylene oxide – chemical gasses use for sterilization b. Chlorine bleach – disinfectant/ halogens/ destroys protein and membranes c. Penicillin – antibiotics d. Soap and hot water – not strong enough e. Interferon – protein responsible for neutralizing viruses 17. Transcription refers to: a. The synthesis of a new strand of DNA from an existing DNA template b. The synthesis of a new strand of RNA from an existing DNA template Downloaded by Manjot Sawhney (manjotsawhney21@gmail.com) lOMoARcPSD|37627750

Transcription: DNA to RNA , Translation: mRNA to proteins c. The synthesis of a new strand of RNA from an existing protein template d. The synthesis of a new strand of protein from an existing RNA template – translation e. The synthesis of a new strand of protein from an existing DNA template 18. R plasmids are ___________ that contain the genetic information for _____________. a. genes, replication b. DNA molecules, drug resistance c. viruses, interferon d. proteins, conjugation e. More than one of the above F- plasmids: fertility factors, carry the gene to make F-pilus involved in conjugation Vir plasmids: virulence factors, carry genes for toxin production 19. Which of the following is a possible oncogenic virus? a. Human papillomaviruss – oncogenic (skin infection) b. Epstein-Barr Virus – non-oncogenic (blood and lymph infection) c. Hepatitis B – oncogenic (digestive system infection) d. Varicella-zoster virus – non-oncogenic (skin infection) e. More than one of the above 20. A chronic viral infection occurs when: ch.13 a. After the acute period, the virus continues to be present in high numbers for the life of the patient b. After the acute period, the virus becomes dormant and can be reactivated later – latent infection c. After the acute period, the virus remains present but becomes non-infectious d. After the acute period the patient’s immune system eliminates the virus 21. Prions: a. Contain RNA as the infectious agent b. Are easily destroyed by boiling c. Can be cured with antibiotics d. Are easily spread by infectious droplets e. None of the above are true of prions Prions – infectious protein particles, no genetic material (RNA or DNA), linked to several human and animal diseases which is transmissible spongiform encephalopathies. 22. True or False: Although bacterial endotoxin is generally less toxic than bacterial exotoxin, endotoxin can still cause life threatening illness when present in great quantities. a. True b. False Downloaded by Manjot Sawhney (manjotsawhney21@gmail.com) lOMoARcPSD|37627750

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