Lab 3 Acid, Base, Buffer, and pH Assessment

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Danville Community College *

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101

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Biology

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Apr 3, 2024

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BIO 101 Lab 3 Assessment Summer 2021 BIO 101 - Lab 3 Assessment Acid, Base, Buffer, and pH Data Answer the following questions. 1. Acid, Base, and pH Data State your hypothesis [5 pts]: If you add to an unbuffered solution then there would be a significant change versus a buffered solution. Watch Video – Effects of Acids and Bases on Unbuffered Solutions Table 1: Effects of Acids and Bases on Unbuffered Solutions [1 pts per; 6 pts total] Solution initial pH Chemical added To cause shift pH After Adding 5 Drops 1 5.0 HCl 2.6 2 4.8 NaOH 11.1 Watch Video – Making Buffered & Unbuffered Solutions Table 2.1: Ingredients and pH of Buffered Solutions [1.5 pts per; 15 pts total] B-1 B-2 Amount of dH 2 0 Identity and amount of Weak Acid (B1) and Weak Base (B2) HAC 7mL & NaA4 3mL NH4OH 7mL & NH4Ac 3mL Identity and Amount of Corresponding Salt NaA4 3mL NH4Ac 3mL Buffer Volume 10mL 10mL pH of Buffer 4.7 9.0 Table 2.2: Description of C-1 and C-2 Solutions [1.5 pts per; 3 pts total] pH Water and HCl have been added to make C-1 so that the pH of C-1 = the pH of B-1 ( +/- 0.5) 4.5 Water and NaOH have been added to make C-2 so that the pH of C-2 = the pH of B-2 ( +/- 0.5) 8.9 1
BIO 101 Lab 3 Assessment Summer 2021 Watch Video – Adding HCl to Buffered & Unbuffered Solutions Table 3.1: Testing with Strong Acid for pH shift [2 pts per; 24 pts total] Solutions labeled “acid” Initial pH The addition of _3 _ drops of HCl added to each beaker resulted in the Final pH : Absolute change in pH B-1 4.7 4.6 0.1 C-1 4.8 3.0 1.8 B-2 9.0 8.6 0.4 C-2 8.7 2.8 5.9 Watch Video – Adding NaOH to Buffered & Unbuffered Solutions Table 3.2: Testing with Strong Base for pH shift [2 pts per; 24 pts total] Solutions labeled “base” Initial pH The addition of _3 _ drops of NaOH added to each beaker resulted in the Final pH : Absolute change in pH B-1 4.7 4.9 0.2 C-1 4.4 10.8 6.4 B-2 9.0 9.1 0.1 C-2 8.6 10.7 2.1 2. Graph: Absolute change in pH from Table 3.1 & 3.2. You can do one combined or two individual graphs. [15 Points] 3. Analysis: [8 Points] Is there a correlation between the pH level and the solution? Why? - There is a correlation between the pH level and the solution type. Buffer solutions show a limited change in pH, while non-buffered solutions exhibit more distinct shifts. If you could ask another question for this experiment, what would it be? Why? 2
BIO 101 Lab 3 Assessment Summer 2021 - How do you know what the corresponding salt is? I was confused on that question in Table 2.1 and could not figure out what it could possibly be or if what I put as my answer was correct. B-1 C-1 B-2 C-2 0 1 2 3 4 5 6 7 0.1 1.8 0.4 5.9 Table 3.1 Adding HCl Solutions Labled (Acid) Absloute change in pH B-1 C-1 B-2 C-2 0 1 2 3 4 5 6 7 0.2 6.4 0.1 2.1 Table 3.2 Adding NaOH Solutions Labeled (Base) Absolute change in pH 3
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