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CELLULAR RESPIRATION & FERMENTATION
INTRODUCTION
Yeast are unicellular organisms in the kingdom Fungi
. Yeast have eukaryotic cells
with a large central vacuole that is involved in degradation of macromolecules by hydrolysis. They also have a cell wall for support which is made of the carbohydrate, chitin. Yeast perform cellular respiration in the presence of oxygen, but follow the pathway of alcoholic fermentation when oxygen is absent. Let’s review the structure of a eukaryotic cell!!
LEARNING OBJECTIVES:
1.
Identify the parts of a yeast cell.
2.
Observe videos and plot and graph data of yeast fermentation with different food sources.
3.
Compare the pathway of fermentation with cellular respiration.
BIO 181 online
Yeast cell
VIRTUAL EXPERIMENTS
You will have 3 different assignments today. You will not be able to run the experiment at home, but you will be given some websites to look at, and you will be given data that was collected by previous Bio 181 students. Using this data, you will be able to fill in all the tables and graphs.
VIRTUAL EXPERIMENT # 1: Fermentation in Yeast
1.
Look at this video on You Tube
(2 minutes) on how Yeast Fermentation makes beer.
2.
Then fill in the blanks below on the process of fermentation. You may need to use
your textbook. Answers (each answer used only once): ATP, Ethanol, Glycolysis, Lactic acid, NAD+, Pyruvate, carbon dioxide
a.
Prior to fermentation, glucose is broken down in a metabolic pathway called Glycolysis.
b.
ATP
is the final carbon-product of glycolysis.
c.
When pyruvate is oxidized in animal fermentation
, it produces a carbon product of lactic acid
, but in yeast fermentation
, the carbon products are Carbon dioxide
and ethanol.
d.
Fermentation in both animal cells and yeast oxidizes NADH to make NAD+.
e.
Producing NAD+ allows glycolysis to continue and to make two pyruvate
per glucose in substrate-level phosphorylation.
Part
1:
BIO 181 online
Food source Water (Control)
Food source
Sucrose (table sugar)
Food source
Fructose (fruit sugar)
Time (minutes)
CO2 produced (cm) CO2 produced (cm)
CO2 produced (cm)
0
0
0
0
5
0.1
1.9
1.3
10
0.1
3.8
2.8
15
0.2
6.8
4.1
20
0.2
8.7
5.4
25
0.2
10.8
7.0
Measuring fermentation in yeast.
1.
Look at this You Tube video (2:25)
to get an idea how the experiment with fermentation tubes is done. 2.
Different mixtures were made up in beakers and used to fill fermentation tubes. 3.
The size of the gas bubble was measured in cm and the data recorded in table 1.
Table 1: Yeast
Fermentation measured as Carbon Dioxide Bubble Size (cm) using different sources of food.
BIO 181 online
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Related Questions
Chemolithotrophs meet their energy needs by oxidizing organic food molecules.
True
False
Which of the following events does not occur during the energy-investment phase of glycolysis?
Hexokinase catalyzes the transfer of a phosphate group from ATP to glucose to form glucose-6-phosphate.
Phosphofructokinase catalyzes the isomerization of glucose-6-phosphate to fructose-6-phosphate.
Fructose-bisphosphate aldolase cleaves Fructose-1,6-bisphosphate into two different three-carbon sugars.
Dihydroxyacetone phosphate can be converted to glyceraldehyde-3-phosphate with the help of the enzyme triose phosphate isomerase.
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Yeast produces carbon dioxide gas. Therefore, based only on this information, can we tell whether the yeast were using aerobic cellular respiration or Anaerobic (ethanol) fermentation? Yes or No
If not, what additional information would we need to know to tell whether the yeast used aerobic respiration or anaerobic (Ethanol) fermentation?
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is the enzyme mechanism used by lysozyme
is the enzyme mechanism used by carbonic anhydrase
is the name of the enzyme mechanism used by chymotrypsin
is the name of the enzyme that cleaves polysaccharides that give structural integrity to bacterial cell wall
is the name of the amino acid other than aspartic acid that is present in the active site of lysozyme
is the enzyme classification where lysozyme belongs
is the optimal pH for the action of lysozyme
is the name of the enzyme that catalyzes the hydration of carbon dioxide to bicarbonate
is the name of the metal present in the active site of carbonic anhydrase
is the pka of water when its is bonded to the metal ion in the active site of carbonic anhydrase
arrow_forward
Which of the following processes is anaerobic?
alcoholic fermentation in plants only
lactic acid fermentation in animals only
glycolysis only
only alcoholic fermentation in plants and lactic acid fermentation in animals
alcoholic fermentation in plants, lactic acid fermentation in animals, and glycolysis
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Clecese
Civcelysis
Pyrevate
rentation
Lactic
Acid
Ethyl Alcohol
and CO:
Acety1-CaA
Mitochondrion
Citric Acid Odle
(Kreb's Cycle)
2 ATP
Electron
Transport Chain
32 ATP
The greatest amount of ATP will be produced in a cell when
when lactic acid is being made
the reactions of the cytosol take place
oxygen (O2) is being used by the cell
O oxygen (O2) is not being used by the cell
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a. What is the purpose of boiling the wort in beer preparation?b. What are hops used for?c. If fermentation of sugars to produce alcohol in wine is anaerobic,why do winemakers make sure that the early phase of yeastgrowth is aerobic?
arrow_forward
Trypanosoma brucei is a parasitic protozoan that causessleeping sickness in humans, Transmitted by the tsetse fly,sleeping sickness is a fatal disease characterized by fever,anemia, inflammation, lethargy, headache, and convulsions.When trypanosomes are present in the human bloodstream,they depend on glycolysis entirely for energy generation.The first seven glycolytic enzymes in these organisms arelocalized in peroxisome-like organelles called glycosomes,which are only regulated weakly by allosteric regulatormolecules. Glycosomes take up glucose and export glycerate3-phosphate. There are two pools of ADP and ATP(cytoplasmic and glycosomal), and the glycosomal membrane is impermeable to both nucleotides as well as mostother glycolytic intermediates. If the glycosomal membraneis compromised, the concentration of phosphoylated glycolytic intermediates rises and the cells die. Explain.
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[Glycolysis and Fermentation in Yeast]
Answer in 2 - 3 sentences.
#1:
Relate differences in the fermentation of different carbohydrates to the natural environment of yeast.
#2:
Explain why the production of CO; in your yeast experiments was mostly the result of fermentation rather than aerobic respiration.
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What is the primary difference between lactic acid fermentation and alcoholic fermentation?
lactic acid produced CO2, whereas alcoholic fermentation does not
alcoholic fermentation produces O2, whereas lactic acid does not
alcoholic fermentation produces CO2, whereas lactic acid does not
alcoholic fermentation is an aerobic process, whereas lactic acid fermentation is an anaerobic process
arrow_forward
10. Compare and contrast fermentation
to cellular respiration. Click and drag the
following to the correct heading:
Oxygen
Glycolysis
Lactic acid
Require 2 ATP
to start
Cytoplasm
only
Pre-work 3 - Video 3
Krebs
Cycle
aerobic
No
Oxygen
Cytoplasm
and
mitochondria
anaerobic
Electron
Transport
Chain
Pyruvate
ethanol
36 - 38 ATP
made
Production
of CO2
NADH →
NAD+
Cellular
Respiration
Similar
Fermentation
Pre-work
1
Live
Session 1
Pre work 2
Live
Session 2
Weekly
Summary
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Remember that the chemical energy stored in glucose is found in the chemical bonds. Explain why aerobic
cellular respiration releases much more energy for the cell to use than anaerobic.
anaerobic cellular respiration examples
Lactic Acid Fermentation
20,H,O, + 2 ATP
Alcohol Fermentation
2C,H,OH + 2CO, + 2 ATP
aerobic respiration
tv
Aa
MacBook Air
80
888
DII
DD
F2
F3
F5
F7
F8
F9
F10
F11
23
$
&
*
3
4
5
7
8
E
R
Y
U
{
D
K
L
C
V
M
.. .-
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Describe each step of the metabolic pathway
shown in the image below.
Linoleic Acid Metabolism
18:2n-6,
HO.
Linoleic Acid
18:3n-6
он
Gamma Linolenic Acid
20:3n-6
он
Dihomo Gamma Linolenic Acid
20:4n-6
HO,
Arachidonic Acid
22:4n-6
но,
Docosatetraenoic Acid
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Fill out the table Energy Carriers Produced in Glycolysis The transition step and the kreb cycle
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Fermentation will occur
under anaerobic conditions only Sometimes the condition is temporary, resulting in lactic acid fermentation Once an oxygen debt has been repaid, fermentation will stop
under anoxic conditions only Sometimes the condition is temporary, resulting in lactic acid fermentation Once an oxygen debt has been repaid, fermentation will stop
under anoxic or anaerobic conditions Sometimes the condition is temporary, resulting in lactic acid fermentation Once an oxygen debt has been repaid, fermentation will stop under anoxic or anaerobic conditions Sometimes the condition is permanent, resulting in lactic acid fermentation
arrow_forward
Why does a chemoautotroph sometimes need to run its electron transport chain backwards?
To generate ATP which is not made when the transport chain is run forwards
To generate proton motive force which is not made when the transport chain is run forwards
To generate NAD+ which is not made when the transport chain is run forwards
To generate NADH which is not made when the transport chain is run forwards
To generate glucose which is not made when the transport chain is run forwards
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Transport Chain
5. Define cellular respiration. What is the difference between aerobic and anaerobic cellular
respiration? Clearly define the proton motive force and chemiosmosis.
6. Summarize the process of fermentation. Describe 2 different fermentation processes
Fermentation
in the
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Word Bank
2 Acetyl-CoA
2 ATP
2 ATP
34 ATP
2€0€
4 CO₂
Electron transport chain
2 FADH₂
Fermentation
Glucose-
Glycolysis
Krebs cycle
Lactic Acid
2 NADH
-2 NADH-
6 NADH
NADT
2-Pyruvate
Fermentation
whose products include
↓
Lactic Acid
and regenerates
Cellular Respiration
begins with
CO
NADE
Glucose
In the absence of 0₂
can be used in
which is broken down during
Glycolysis
1
which produces
↓
2 Pyruvate
2002
2ATP
+
yielding a
net gain of
2NADH
In the presence of 0₂
can be used to produce
+
which is catabolized via the
Krebs cycle
with a net yield of
used in the
Electron transport Chain
to produce
34ATP
used in the
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Please explain why each choice is wrong or correct.
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Kombucha requires two fermentation steps. The first one is partly aerobic and partly anaerobic. The aerobic growth occurs because the kombucha is allowed to brew exposed to oxygen. Where is the anaerobic growth occurring in the first fermentation step?
The second fermentation step is the step in which we add the kombucha to a sealed bottle therefore I'm confused as to where it can occur before we seal it.
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V.
Respiration in eukaryotes is completed in four stages with varying amounts of ATP
molecules produced as shown in the table given below. Study the table and answer the
question.
Stages of respiration
1. Glycolysis
2. Link reaction
3. Krebs cycle
4. Oxidative phosphorylation
ATP molecules Net gain of ATP
produced
molecules
1
6
0
6
2
24
0
8
Which of the following stages is correctly matched?
A
1 and 2
B
2 and 3
C
3 and 4.
D
4 and 1
vi.
The figure shows the reflex arc of a person having perceived stimulus from the
environment. The correct path of the conduction of nerve impulse is
arrow_forward
Also this alcohol fermentation has stumped me. I'm not sure if how the question is being asked :(
arrow_forward
Choose the odd one for fermentation
It occurs in prokaryotes and unicellular eukaryotes only
It occurs in absence of oxygen
Net gain of only two molecules of ATP from each molecule of glucose
There is no any net gain of NADH + H+
arrow_forward
Look at the cellular respiration reaction. What reactants and/or catalysts (enzymes) does the yeast supply? What does the juice supply?
What is the product of respiration that we need? In other words, why do organisms need cellular respiration?
Propose an alternative experiment to test rates of cellular respiration besides measuring acid production (hint: look at the reaction equation).
Do some reading and discuss some applications of fermentation in biotechnology, food production, or medicine.
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None
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Picture. 1: Fresh potato +H202 H2O+02 (air bubbles)
Questions:
1. Why did you use buffer instead of distilled water to dilute the enzyme and the
substrate?
2. What do we mean by enzyme specificity?
3. Name the substrate of peptidase, sucrase and amylase?
4. Discuss the factors affecting the rate of an enzymatic reaction, showing how each
one affects the rate?
34
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There are a few things that go along with eukaryotes which are, plants, fungi and protozoa. The net ATP yield for eukaryotic cells are 2 ATP moving through membranes, however, in other words only have 36 ATP.
How many chemical reactions (steps) are involved? Briefly describe them.
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Plasmodium use digestive vacuoles (DV) to digest hemoglobin and heme from red blood cells into fuel sources for their life cycle. The DV is the site where host proteins are degraded by the parasite and then the peptide pieces are exported into the Plasmodium cytosol for use in biosynthesis. Plasmodium falciparum chloroquine resistance transporter, PfCRT, is a transporter in this DV membrane and its original function was unknown until recently. Researchers digested hemoglobin into peptide fragments and then tested the effect of these fragments on PfCRT function. VF-6-2 is one of these fragments of hemoglobin and apparently a natural substrate of PfCRT. Chloroquine, for which the transporter was originally named, is a substrate only of mutant transporters. One mutant PfCRT, for instance, has K76T in its substrate binding site and thus a significantly lower Km for chloroquine, inspiring the original name of the transporter, because of its importance for drug resistance in malaria…
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Similar to the activity in our muscle cells, certain bacteria canutilize sugars by aerobic cellular respiration (which requiresoxygen) or by fermentation (which does not). However, like musclecells, these bacteria only use fermentation when oxygen is notavailable. Why is aerobic respiration preferable? What is thespecific role of oxygen in this process?
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- What is the primary difference between lactic acid fermentation and alcoholic fermentation? lactic acid produced CO2, whereas alcoholic fermentation does not alcoholic fermentation produces O2, whereas lactic acid does not alcoholic fermentation produces CO2, whereas lactic acid does not alcoholic fermentation is an aerobic process, whereas lactic acid fermentation is an anaerobic processarrow_forward10. Compare and contrast fermentation to cellular respiration. Click and drag the following to the correct heading: Oxygen Glycolysis Lactic acid Require 2 ATP to start Cytoplasm only Pre-work 3 - Video 3 Krebs Cycle aerobic No Oxygen Cytoplasm and mitochondria anaerobic Electron Transport Chain Pyruvate ethanol 36 - 38 ATP made Production of CO2 NADH → NAD+ Cellular Respiration Similar Fermentation Pre-work 1 Live Session 1 Pre work 2 Live Session 2 Weekly Summaryarrow_forwardRemember that the chemical energy stored in glucose is found in the chemical bonds. Explain why aerobic cellular respiration releases much more energy for the cell to use than anaerobic. anaerobic cellular respiration examples Lactic Acid Fermentation 20,H,O, + 2 ATP Alcohol Fermentation 2C,H,OH + 2CO, + 2 ATP aerobic respiration tv Aa MacBook Air 80 888 DII DD F2 F3 F5 F7 F8 F9 F10 F11 23 $ & * 3 4 5 7 8 E R Y U { D K L C V M .. .-arrow_forward
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