Week 4 Lab 3 Microbiology Pre-Lab

plz answer all the questions plz and also state the aim for this lab .. thank u

LABORATORY REPORT: Aseptic Technique & Inoculation of Bacteria Guide Questions. 1. Why is direct flaming preferred when disinfecting loops and needles? 2. Why is it important to flame the entirety of the loop and not just the tip? What consequences can be seen when this process is not done correctly? 3. What is the difference between quadrant streak method A from method B? 4. Why do we use a pencil instead of a pen when labelling culture tubes or plates? Are there other alternatives in labelling? 5. Why is an inoculating needle used in Subculturing Techniques? Can a loop be used instead? NOTE: Please try to answer all of the question asked, i promised to give you a good ratings

Task 2 - number 1. Check the following papers, each of which includes an excerpt of their observations and is quoted using the Chicago manual format. Based on the given research title and research general objective, write R if the given research is relevant, credible, and up to date. Otherwise, use the letter N.

Question:- Describe control strains used in the clinical microbiology laboratory and explain their maintenance in the laboratory. ( write BY WORD and all steps I need). Introduction Discussion References

ASSIGNMENT # 4 A 36 YR OLD FEMALE PRESENTED WITH MASSIVE EDEMA OF BOTH LEGS. SHE SAYS THAT SHE HAS BEEN WORKING IN THE FIELDS FOR 4-5 YEARS. AT FIRST SHE GOT A SMALL ITCHY RED PATCH ON HER FOOT AND AFTERA YEAR HER LEGS STARTED SWELLING. NOW SHE HAS DIFFICULTY WALKING. HER CONDITION IS SHOWN BELOW. Q1. Name the insect vector responsible for causing this disease. Q2. Name the phases involved in the life cycle of this organism. Q3. Who is the definitive and intermediate host? Q4. Name the stage in which it infects humans. Q5. NAME THE TREATMENT OPTIONS AVAILABLE?

List each step of learning goals that was listed from the picture

ANSWERS TO QUESTIONS ON LABORATORY ASSAY NO.2 1. Why is it necessary to calibrate a Pasteur pipette? 2. Cite some possible sources of errors in the calibration of Pasteur Pipette. 3. Cite other procedural techniques that can be used for the calibration of Pasteur Pipette.

Need help figuring out the top two CFU questions. Unsure of how to calculate

Minute Timed PX B 4 5 6 rutgers.instructure.com/courses/207533/external_tools/retrieve?display=full_width&url=https%3A%2F%2Frutgers.quiz-Iti-iad-prod.instructure.com%2Flti... 7 8 9 10 11 12 13. 3 Quizzes 2 X Previous + 01:18:44 < Time Remaining Return 1 point The PCCE of the respiratory tract helps capture inhaled debris and pathogens which are then absorbed into the bloodstream and filtered out by the liver for elimination through the digestive system. True False U Nex d Next

Case Study #4   Setting: Warsaw Bielany Hospital in Warsaw, Poland.   Subjects: 2522 urine cultures from children admitted to the Department of Pediatrics with varying severities of urinary tract infections from January 2004 to December 2006.   Quantitative analysis was performed on the samples collected and the results analyzed. The results follow for one of the three most commonly identified organisms that were found in the collected samples...   Key to Media Used in Case Studies (in picture left to right) Phenol Red Glucose Phenol Red Sucrose Results holds for lactose too Phenol Red Xylose SIM Read as Sulfur / Indole Lysine Decarboxylase Ornithine Decarboxylase Methyl Red Vogues-Proskauer Urea Simmon’s Citrate Nutrient Gelatin   Results of Inoculated Media   A B C D E F G H I J K Based on the results of the media shown above, determine the causative agent of the infection. Use the figures in this presentation or in your lab manual for identification.

Subject : Environmental Microbiology  Can u use the information given below to answer these 2 question  1.Provide an aim for this lab 2. Provide objectives  DISCUSSION QUESTIONS   What is the relationship between the resolution power and the useful magnification that may be obtained with the light microscope?  What determines the resolving power of the lens system?                                What is the limit of resolution obtainable with the light microscope?                                                 How you will distinguish between bright field and dark-field microscopy and provide a specific example where each would be method of choice for observing a culture of bacteria?    What advantages does electron microscopy have over light microscopy?    What are disadvantages of electron microscopy over light microscopy?                               #Compare the use and the methodology of TEM with SEM? Provide at least one example where each would be the method of choic

Give the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions Picture   1. Microscope     2. Colony Counter     3. Autoclave     4. Microbiology incubator     5. Drying oven     6. Refrigerator (microbiology)     7. Bunsen burner/alcohol lamp     8. Candle jar     9. Anaerobic jar     10. Microhood or Bacteriologic hood/Safety hood/Safety cabinet

MODULE 5 BACTERIOLOGY CASE STUDIES Instructions: Read the following cases and answer the questions accordingly. Type your answer after each question. Case 1 A 34-year old male patients arrives at a local hospital. He felt completely unwell, has a fever, and he lost over 7 kg in the last month. He also suffers from a severe cough with rust-colored sputum. His doctor requested for a chest X ray and sputum sample. During interview, it was revealed that the patient tested positive for HIV a year ago. A tuberculin skin test is then ordered. For three years, he had been living with two roommates. Before that, he lived with another roommate who had tested positive for TB 6 months prior to onset of the patient's symptom. Laboratory tests were also ordered for his two roommates and the results are summarized below: Roommate 1 Roommate 2 Sputum HIV Negative Negative Positive Negative Negative Positive Tuberculin test 1. Why was chest X ray and sputum sample ordered? 2. Based on the symptoms and…

Direction: Read and analyze the following laboratory experiment and answer the following question. PART 1: SURFACE AREA AND CELL SIZE Materials: Agar containing NaOH, and the pH-indicator dye phenolphthalein cured into cubes of various size, 3 plastic cups, HCl, metric ruler, paper towels. Methodology: 1. Safety: Wear goggles and nitrile gloves while completing this lab. 2. Obtain three different size blocks of pink or blue agar. Using a ruler, measure the length, width, and height of the three blocks given below. Cut the agar according to the given dimension. Small = 1 cm x 1 cm x 1 cm Medium = 2 cm x 2 cm x 2 cm • • Large = 1 cm x 1 cm x 6 cm 3. Record your data. 4. Pour HCl or vinegar into two small cups. Place the one larger "cell" into one cup and the two smaller cells in the other cup. Start timing 30 minutes. 5. After 30 minutes, remove the cells and blot them dry with a paper towel. 6. Using your ruler, measure the distance the HCl has diffused into the blocks as shown on the…

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Answer 6-7 sentences each. pleaseee badly need it ASAP State the importance of familiarizing with the different equipment and apparatuses used in microbiology laboratory. Enumerate 5 precautionary measures to be observed to ensure safety when using various instruments used in the laboratory.

I need questions 2 and 3 under post-lab answered

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SURG200 Ch. 10 Case Study   A surgical technology student is completing rotation at the clinical site’s central sterile processing department. The preceptor notifies the student that a patient has just arrived in the emergency department with a gunshot wound to the chest, and the student needs to assemble a case cart containing everything that will—or might—be needed for the case. The patient will be in the OR in 10 minutes, so the student must quickly work.   What instrument trays should be gathered for this case?   What equipment must be in the room for this case?   What supplies must be available for this case?   Considering the extremely limited amount of time available, what should be opened first, and what can be opened as the case progresses?  Please only answer questions 1 and 4.

Complete both pages correctly

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Give the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions Picture 6. Refrigerator (microbiology)     7. Bunsen burner/alcohol lamp     8. Candle jar     9. Anaerobic jar     10. Microhood or Bacteriologic hood/Safety hood/Safety cabinet     11. Bacteriologic filters (Seitz, Chamberlain, Berkfield)     12. Petri dish     13. Culture tubes     14. Hanging drop slide     15. Durham’s tube

l JO 100 3G 10:41 PM Field Training Clinical Microbiology a Tau, aliu uistuss tne principit anu tiie ust uI VITEK Z COupact, AuLOmaleu ID/AST Instrument, and Bact/ALERT automated Blood Culture Systems. Question #2: 45-year-old male had been working in his garden when he scratched the knuckle of his right arm during digging in ground with a spade, he immediately washed the area but noticed later that night that the knuckle became red, warm to the touch and swollen. He went to the hospital, the doctor ordered a Gram stain and culture on the pus extracted from the wound. The culture grew the following organism and the Gram stain from the wound showed the following results: Based on the previous results, answer the following questions: 1. As a microbiologist, describe what you see on the Gram stain taken from the wound site. And give a preliminary report on what you see on the culture plates. What is the probable Genus and species? 2. Name 2 tests that can be done to positively identify…

Activity 3: Short Essay Directions: Choose two questions to answer. You may use a separate sheet to complete the task. 1. Ascorbic acid (Vitamin C) is a soluble vitamin in the human body. Justify your answer. 2. Why can termites eat wood barks and other thick and hard parts of plants? Explain your answer. 3. Is using steroids good in the human body? Why or why not? 4. What is the difference between DNA and RNA?

Pre-lab task 1: on the other side of this page, note the size range for typical Escherichia coli cells (length and width) and for most species of Anabaena (length and width). Be sure to include NLM/CSE-formatted references for the source of this information.

POST-LAB QUESTIONS 1) Discuss how the following buffer systems work in the human body: a) Carbonate buffer system b) Phosphate buffer system c) Proteins

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Give the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions Picture 1. Microscope     2. Colony Counter     3. Autoclave     4. Microbiology incubator     5. Drying oven

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Activity 2. Media Used in Isolating Coliforms You are a group of microbiologists tasked to test the presence of coliforms in the newly built water station. You are asked to isolate E. coli, Salmonella, and Shigella. Enumerate the media used for each step of isolating these bacteria. Include pictures and references. Bacteria Enrichment (Broth/Agar) Presumptive test (Broth/Agar) Isolation Media (Broth/Agar) E. coli Salmonella Shigella 1. How can you confirm that E. coli, Salmonella, and Shigella are present in the newly built water station? Explain. 2. What is the difference between nutrient broth and nutrient agar? 3. What is the importance of these steps (Enrichment, Isolation, and presumptive test) in isolating bacteria? Conclusion about the process of isolating bacteria