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Which of the following statements regarding electron microscopy is FALSE?   Question options:   live organisms cannot be viewed with an electron microscope   all of these statements are TRUE   scanning electron microscopes focus electrons across the surface of a sample   electrons pass through a sample viewed using transmission electron microscopy   electron microscopes use magnetic fields in place of glass lenses   electron microsopes have a better resolving power than light microscopes

True or false?       light microscopy has a limit of resolution of 200nm.   phase contrast microscopy can be used to observ thick (more than 2 cells)speciments.     mammalian cells are grown in a humidified incubator at 30C and 5% CO2.

3D dimensionality is a limitation of the compound microscope. Depth of field, DOF, describes dimensionality form top to bottom and can be observed with colored cross threads. Observe the crossed thread slide on low power (4x), then on medium power (10x), then on high power (40x objective magnification). Which crossed fiber is on top? How do you know?

Copy and paste the link below and watch the video on Youtube https://www.youtube.com/watch?v=8RBs0Ghg_48   Answer the following Questions: 1. What are the chemicals and materials used in gel electrophoresis?   2. Draw a schematic diagram of a gel electrophoresis set-up 3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam?   4. What is the use of ethidium bromide and why must you wear gloves when you handle it?   5. What makes the DNA fragment move towards the positive plate?   6. What is the purpose of glycerol in the sample buffer?    7. What is the use of a DNA ladder?    8. What will happen when you increase the voltage of the set-up?   9. Can gel electrophoresis be used to separate amino acids? If so, how is it done?

PART C: CALCULATING THE DIAMETER OF THE FIELD OF VIEW (FOV)_ The field of view (FOV) is the circular area you can see when you look through the microscope. The diameter of the field of view is different depending on which objective lens you are using. For example, you are using the medium-power objective lens, then the area you can see is actually smaller than if you were using the low-power objective lens. Knowing The diameter of the field of view can help you estimate actual size of objects / cells seen through the microscope. When the revolving nose piece is turned to the low power objective lens, a dear plastic ruler can be placed on the microscope stage (see figure 1). Then, the coarse adjustment knob can be used to focus on the millimeter marks of the ruler making sure that one of the milimeter marks is at the left edge of the field of view (see figure 2). NOTE: Slage cip I-1000 Objects in the FOV are usually measured in micrometers (um). To convert, a FOV in mm, times it by 1000…

Saved Sort the following characteristics based on whether they apply to light or electron microscopes. 2000x 1,000,000x or more 200 mm (0.2um) Yes No Usually not 0.5 nm Table 3.3 Comparison of Light Microscopes and Electron Microscopes Characteristic Light or Optical Electron (Transmission) Useful magnification 200 mm (0.2µm) 1,000,000x or more Maximum resolution 200 mm (0.2um) 1,000,000x or more Image produced by Light rays Electron beam Image focused by Glass objective lens Electromagnetic objective lenses Image viewed through Glass ocular lens Fluorescent screen Specimen placed on Glass slide Copper mesh Specimen may be alive Yes Specimen requires special stains or treatment Depends on technique Yes Shows natural color Reset

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The most widely used type of light microscope is the Multiple Choice bright-field microscope. dark-field microscope. phase-contrast microscope. confocal microscope. fluorescence microscope.

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Clinical microscopy How to troubleshoot a microscope? How to cleanup a microscope? How to take care a microscope?

Animal Cell (Ideal Model) This is a model of an ideal animal cell. Objects are not to scale and many of them would not be visible with a compound light microscope. Fill in the blanks.

Distinguish scanning tunneling from atomic force microscopes in terms of how they create images and their uses

Simulated data: Imagine you collect the following images at the above magnifications. Figure 3-2: Images of cheek cells at the above magnifications. Labelling Drawing: Label only the microscope image taken at 1000x magnification. Add lines and labels (as per guidelines for scientific drawings). You should include: A scale bar showing a unit length of 20 µm. (Hint: The length of the human cheek cell shown is 60 um). Labels for the cell membrane, nucleus, and cytoplasm.

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Which of the following method is employed in the sample preparation for the cryo-electron microscope? O The sample is flash-frozen in water to form crystalline ice O The sample is embedded, sectioned and then stained with a negative stain then flash-frozen in water to form vitreous or amorphous ice The sample is flash-frozen in water to form vitreous or amorphous ice The sample is embedded, sectioned and then stained with a negative stain then flash-frozen in water to form crystalline ice

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Which of the following microscopes typically requires the use of vital dyes (like methylene blue) to visualize large subcellular structures in a living cell (like the nucleolus or the mitochondrion)? the scanning/tunneling electron microscope (STEM), with good resolution up to about 100,000,000x the transmission electron microscope (TEM), with good resolution up to about 100,000x the scanning electron microscope (SEM), with good resolution up to about 1,000,000x the compound light microscope, with good resolution up to about 1,500x all of the above microscopes would be equally useful in visualizing the interior of organelles

Explain when to use bright-field, phase-contrast, dark-field, fluorescence, transmission electron, and scanning electron microscopy for a given situation. What is an example of this situation?

You may want to use this resource for this problem. If you do, submit the output along with your solution.You have been given a confocal microscope equipped with the following lasers, excitation filters, andemission filters:Laser Emission filter355 nm 410-470 nm405 nm 470-500 nm488 nm 500-550 nm532 nm 570-610 nm561 nm 610-650 nm640 nm 660-700 nm808 nm 720-780 nmYour task is to design an experiment to visualize the following:1. Nuclei2. A fluorescent protein in the cytosol3. A cell membrane marker antibody conjugated with a fluorophore4. Actin filaments5. LysosomesYou may choose from the following fluorophores for each of the five channels:Nuclei Fluorescent protein Membrane marker Actin marker Lysosome trackerDAPI GFP FITC AF488 Phalloidin LysoTracker RedHoechst 33342 YFP WGA-TRITC AF568 Phalloidin LysoTracker DeepRedSYTO Deep Red RFP Cy7 AF594 Phalloidin LysoTracker Blue   Part 3.1Choose appropriate fluorophores for each of the subcellular structures to be imaged, taking into…

To visualize individual atoms in a protein or nucleic acid you would use: X-ray crystallography  fluorescence microscopy  poly acrylamide gel electrophoresis  transmission electron microscopy  light microscopy

What description best describes the compound light microscope? Uses a beam of electrons is transmitted through a specimen to form an image. Has more than one lens and its own light source. In this type of microscope, there are ocular lenses in the binocular eyepieces and objective lenses in a rotating nosepiece closer to the specimen. An optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. Designed for low magnification observation of a sample, typically using light reflected from the surface of an object rather than transmitted through it.

Discussion: 1) What does calibrating the microscope mean? Why do we calibrate the microscope? 2) What is the ocular calibration scale for each objective? Why is important to calibrate each objective?

There are many different types of microscopes that can be used in science. Research the function of each type of microscope below  Stereomicroscopes Bright field microscope Dark field microscope Fluorescence microscope Phase contrast microscope Nomarski microscope Scanning electron microscope Transmission electron microscope

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Does it reduce, deaden, or enhance human creativity using Titan 80-300 Cubed Microscope?

Both light and electron microscopy are commonly used to visualize cells, cell structures, and the location of specific molecules. Explain why a scientist may choose one or the other microscopy technique for use in research.

Discuss the microscopy technique that gives the best contrast image of non-biological samples such as micro and nano-particles.

Please answer fast what are some tips to improve image quality when looking at a organsims using a microscope

For each type of microscopy, know how each is used and the type of image produced. kEEP IT SHORT AND SWEET        Light microscope –        Transmission electron microscope -        Scanning electron microscope –

Microscope You should always position the cells or tissues in the center of view before increasing magnification, because you know that the image will move right as you move the slide to the left. you know that the image will move up as you move the slide down you know that the center of field of view will be unchanged this is the best way to avoid breaking the slide with an objective