Copy of Fermentation Experiment

I am needing help with questions 1 through 3, please.  1) What is the importance of the Control Group in the Yeast and Sugar Balloon Experiment? 2) Write out the general summary equation for alcohol fermentation below using the formula for glucose; label the substrates and the products in this equation. 3) Research: Initial background about yeast and fermentation- write a few sentences about yeast and fermentation based on what you know. In what situations do humans use yeast for fermentation?

Table 3 - Determination Tube # Potato Expt. Temp. 2c 3c 4c 5c extract (mL) 2 2 2 2 room temp 20 °℃ 40 °℃ 75 °℃ Boiling 100 °C of the optimum temperature of catechol oxidase enzyme. 1st Absorbance 0 min. at Expt. Temp. dH₂O Catechol (mL) (mL) 0 0 0 0 13 13 13 13 Start Time: 4:20 Absorbance: 0.072 Start Time: 4:25 Absorbance: 0.114 Start Time: 4:25 Absorbance: 2nd Absorbance after 10 min. in Expt Temp. Absorbance: 0.097 Time for reading: 4:30 Absorbance: 0.128 Time for reading: 4:33 Absorbance: Subtract 1st from 2nd absorbance 0.056 0.137 Time for reading: 4:35 Absorbance: 0.132 0-193 Start Time: 4:27 Time for reading: 437 Absorbance: 0-126 0.023 0.061 0.029 3rd Absorbance after 10 more min. at Room Temp. Time for reading: 4:40 Absorbance: Based on the data from Table 2 answer these questions: Q10) What is the enzyme's optimum temperature? Answer: Q11) Use the difference between 2nd and 1st absorbance to support your conclusion for the optimum temperature. 0.159 Time for reading: 4:43…

EXPERIMENT : UTILIZATION OF FOOD/AGRICULTURAL WASTE FOR PRODUCTION OF BIOETHANOL OBJECTIVE To learn on the basic process of microbial fermentation. To quantify the conversion of agricultural waste to bioethanol. Procedure: The agricultural wastes were got and then blended for 3 min. The juice was extracted by filtering using a double-fold of cotton gauze cloth, and particulate matter was removed by centrifuging at 10,000 g for 10 min at 4°C. The undiluted juice was stored, and the waste material obtained from the extraction of juice at -20°C until use.   Kluyveromyces marxianus on the Yeast-Malt (YM) agar containing 10 g/L glucose, 5 g/L peptone, 3 g/L yeast extract, 3 g/L malt extract, and 20 g/L agar were maintained.   Culture was prepared by inoculating two loopfuls of the fungus in a 250 mL Erlenmeyer flask with 100 mL growth medium containing 30 g/L glucose, 5 g/L yeast extract, 2 g/L NH4Cl, 1 g/L KH2PO4, 0.3 g/LMgSO47H2O. The preculture was grew on a rotary shaker at 150…

EXPERIMENT :UTILIZATION OF SOLID WASTE FOR PRODUCTION OF VALUE-ADDED PRODUCTS OBJECTIVES  : To apply techniques/ technologies in waste management system for production of value-added products PROCEDURES: Separate the peels of different fruits were separated and oven dried at 50°C, ground and sieved through mesh screen. 50 ml of 10% (w/v) HCl was added to each waste (40 gm) in conical flask respectively. The mixture in water bath was placed at 100° C for one hour. The mixture was cooled down and the mixture was filtered through Whatman filter paper. The filtrate was diluted with sterile distilled water at varying concentration. The fruit hydrolysate was autoclaved at 121°C for 15 minutes. Saccharomyces cerevisiae was maintained on the YDPA at 28 °C. In 250 mL conical flasks, each of 100 mL of various fruit hydrolysate growth media supplemented was added with (NH4)2SO4 (2 gm), KH2PO4 (1gm), MgSO4.7H2O (0.5 gm), NaCl (0.1 gm), CaCl2 (0.1 gm). The initial pH of all the culture media was…

Benedict test

What are the two appropriate control groups if flask 2: ‘Yeast + Glucose + Water’ is your experimental group and what is each missing that flask 2 contains?

Table 3-Determination Tube # Potato Expt. Temp. 2c 3c 4c 5c extract (mL) 2 2 2 2 room temp 20 °C 40 °C 75 °C of the optimum temperature of catechol oxidase enzyme. 1st Absorbance 0 min. at Expt. Temp. Boiling 100 °C dH₂O Catechol (mL) (mL) 0 0 0 0 13 13 13 13 Start Time: 4:20 Absorbance: 0.072 Start Time: 4:25 Absorbance: 0.114 Start Time: Absorbance: 25 0.132 Start Time: 4:27 Absorbance: 0.097 2nd Absorbance after 10 min. in Expt Temp. Time for reading: 4:30 Absorbance: 0.128 Time for reading: 4:33 Absorbance: 0.036 0-137 Time for reading: 4:35 Absorbance: Subtract 1st from 2nd absorbance 0.193 Time for reading: 4:37 Absorbance: 0-126 0.023 0.061 0.029 Based on the data from Table 2 answer these questions: Q10) What is the enzyme's optimum temperature? Answer: Q11) Use the difference between 2nd and 1st absorbance to support your conclusion for the optimum temperature 3rd Absorbance after 10 more min. at Room Temp. Time for reading: 4:40 Absorbance: 0.159 Time for reading: 4:43…

Dichotomous key:b. Based on the key, bacteria of which genus would appear purple after a Gram-staining test? please refer to the attached photo.

RESULTS Please give the time-absorbance values tab? Draw graph-growth curve in Excel? Find the equation of slope line? Calculate the generation time? Choose the values in log phase Abs 0.005 25 0.003 50 0.023 75 0.026 100 0.029 125 0.035 150 0.1 175 0.26 200 0.32 225 0.375 250 0.42 275 0.46 300 0.475 325 0.489 350 0.506 375 0.515 400 0.521 425 0.526 450 0.532 475 0.522 500 0.515 525 0.514 550 0.506 575 0.489 600 0.465 625 0.42 650 0.38 675 0.36 700 0,345 725 0.333 BACTERIAL GRO WTH CURVE

Yeast cells are added to a 3.0 L batch bioreactor so that the initial cell concentration is [X]. = 1.3 g cells / L. The growth mediğim in the reactor, which is well-mixed so the cells have access to all nutrients, contains 150 g CELL DATA ribose (C5H10O5, MW 150), 75 g ammonia (NH3, MW 17), and 85 g oxygen (O2, MW 32). A-10. Determine the maximum concentration of cells, [X], that will form in the bioreactor when the limiting nutrient is consumed. Search Yx/ribose YX/02 YX/NH3 Specific growth rate on limiting nutrient, u Lag phase duration Deceleration phase duration 0.48 g/g 0.87 g/g 0.23 g/g 0.51 h 30 minutes Negligible

If you know anything about the biology can you help me on this yeast fermentation lab worksheet if you did the lab or u know anything about

I am having an incredible difficult time understanding how to do this. This is for Measuring gas bubbles in terms of Ceullar Respiration for yeast fermentation.

In cultivation of E.coli strain in a batch stirred and aerated tank, a static gassing out technique method was employed to determine the oxygen transfer coefficient (k̟a) for the bioreactor. After purging the oxygen with nitrogen gas, continuous air supply at air flow rate of 0.1 v.v.m. and impeller speed of about 250 rpm was initiated. Increase in the dissolved oxygen concentration upon the continuous air sparging is recorded by a dissolved oxygen (DO) probe. Using the following data, determine the oxygen transfer coefficient (ka) for the bioreactor. Saturation DO concentration is C*=9 mg/l Time (minute) Cl (mg/l) 1 1 2 3 2.5 4 3 5 4 6.5 5 7.2

Lab Experiment: Biosynthesis of ethanol Part 2 What is the distillation technique and its uses, equipment to be used and temperature to collect the product If you could answer each part specifically I will appreciate it

this question is not incomplete

Please answer post lab question and give a conclusion about the experiment

coli, mak you believe will grow faster (37° C or 30° C temperature) and why. Results• Time Absorbance 30 °C Absorbance 37 °C Generation time Generation time t=0 min at 30 °C at 37 °C 0.05 0.05 t= 20 min 0.06 0.07 t= 40 min %3D 0.1 0.16 0.28 0.21 0.44 t = 60 min t= 80 min %3D 0.95 Complete the graph. Make sure this figure has a description and all other labels are included. Once you have completed graphing the points, draw a best-fit line for each of your data sets by eye Questions: 1. Which data point can be the first to be considered in log phase for each line? (It will be the first point that seems to be described by your best-fit line.) For the 30° C culture the -minute point is the first one likely to be in log phase. For the 37° C culture the -minute point is the first one likely to be in log phase. Incubation Temperatures - Growth Bio 40- Fall 2019 Page 6

Using the data in the picture below, answer the five questions in the pictures.

1) Which respirometers is the most likely to contain distilled water + yeast (A,B,C,D,E) 2)Which of the respirometers contains carbohydrates that is fermented most efficiently by the yeast (A,B,C,D,E)

After 20 minutes of incubation at 32°C, you measure the bubble height of CO2 gas collected in a respirometer containing yeast as 62.0 mm. What is the volume of CO2 generated and the fermentation rate? Question options: 99.2 mL; 4.96 mL / min 992 mL; 49.6 mL / min 9.92 mL; .496 mL / min .992 mL; 0.0496 mL / min

10_UG_26563_ch09_167-184.indd 175 Press esc to exit full screen Yeast Fermentation The unicellular fungus Saccharomyces cerevisiae is used in the food industry to ferment sugars. The rate of fermentation is measured by CO₂ production. The addition of inorganic phosphate increases this rate. THINK Answer this question individually: 1. Why does the fermentation of sugars, such as glucose and fructose, depend upon the presence of P? PAIR Answer the following questions with a partner: 2. Which glycolytic reactions require P? 3. Several glycolytic intermediates contain phosphate. Where did these groups come from during the reactions? SHARE 4. How much P, is required to generate one CO₂? As a class, discuss the following questions: 5. Is CO₂ generation the most reliable way to measure ethanol fermentation? Why or why not? HANDOUT 175 23/03/17 2:02 pm Page 1 of 4 ...... ZOOM + K

answer the following questions 1.What is the wavelength used for the spectrophotometer? What is the principle of the spectrophotometer? 2.What is the reagent used to detect glucose in the oh and temperature experiments? What is its principle?3.What is the optimum temperature for invertase reactions?4.What is the optimum ph for the invertase reactions?

Based on your results in the table above, determine the species’ ability to ferment glucose or lactose, if carbon dioxide gas is produced as a result, and its ability to produce hydrogen sulfide gas.

Nicotiana tabacum cells are cultured to produce a polysaccharide gum. The reactor used is a stirred-tank reactor with an initial volume of 100 L. The maximum specific rate of growth of the culture is 0.18 d-1 and the yield coefficient of substrate in biomass is 0.5 gX/gS. The concentration of the limiting substrate in the medium is 3% (m/v). The reactor is inoculated with 1.5 g/L of cells and operated in batch until the substrate is exhausted, when the medium is fed with a constant flow rate of 8 L/d. The fed batch occurs in a quasi-steady state condition.a) Estimate the time of the batch step and the concentration of cells reached in this phase, considering exponential cell growth.b) The fed batch phase is carried out for a period of 40 days. What is the final concentration of cells in the reactor?c) The bioreactor is available for the process for 275 days a year, with an interval of 24 hours between each cultivation. What is the most advantageous operating mode for the process…

Laboratory Report Sheet – ENZYMES DATA & RESULTS Potato Peroxidase   Observations potato extract + phenol + H2O2 __________________ potato extract + catechol + + H2O2  ____________ potato extract + Guiac + H2O2 ____________ boiled potato extract + phenol + H2O2 ____________ boiled potato extract + catechol + + H2O2  _________  boiled potato ex tract + Guiac + H2O2  ___________  Note your observations AND does heat destroy peroxidise?

I am needing help with 1 through 3, please 1)   Using the graphs, indicate if the data supports or does not support the hypothesis: The rate of balloon inflation will be directly proportional to the amount of sugar added to the yeast solution. Also, which graph illustrates the most clearly what happened in the experiment and why? 2)  Which graph illustrates the most clearly what happened in the experiment?  Why? 3) Glycolysis in an anaerobic environment produces a net of 2 ATP and Pyruvate.  Why does Yeast bother to ferment Pyruvate to Ethanol?

Dichotomous key: c. Based on the key, which genera contains Gram-negative, rod-shaped bacteria that do not ferment lactose? please refer to attached photo for the key.