Salivary Proteins Procedure Day II

Direction: Answer thoroughly and explain.

Lab Test Comprehensive Metabolic Description Normal Values Clinical Significance if Above/below normal Panel values Albumin: Alkaline phosphatase: ALT (alanine aminotransferase AST (aspartate aminotransferase): BUN (blood urea nitrogen): Calcium: Chloride: CO2 (carbon dioxide): Creatinine: Glucose: Potassium: Sodium: Total bilirubin: Total protein:

Case Study: Case Study: Catalase Activity Catalase H,O, 0 + O2 (2) Catalase is an enzyme that converts hydrogen peroxide (H,O) to oxygen and water. An experiment investigated the effect of temperature on the rate of the catalase reaction Small (10 cm) test tubes were used for the reactions, each containing 0.5 cm of enzyme and 4 cm of hydrogen peroxide. Reaction rates were assessed at four temperatures (10°C, 20°C, 30'C. and 60 C). For each temperature, there were two reaction tubes (e.g. tubes 1 and 2 were both kept at 10°C). The height of oxygen bubbles present after one minute of 10°C 20°C 30°C 60°C Height of охудen bubbles 4 cm H,02+ 0.5 cm erzyme reaction was used as a measure of the reaction rate: a laster reaction rate produced more bubbles. The entire experiment, involving eight tubes, was repeated on two separate days. Tubes Tubes Tubes 1 & 2 Tubes 3 & 4 5 & 6 7 & 8 1. What is the purpose of this experiment? inrestrgatee effect of demperalure on the rate ot he 2. Write a…

Please answer these question. I need these for lab. PRE-LAB ASSIGNMENT – (YOU WILL NOT BE ALLOWED TO BEGIN WORK ON LABWITHOUT THE COMPLETED CALCULATIONS) 1. COMPLETE ALL CALCULATIONS REQUIRED FOR TABLE 1 ( For all measurements, the [PNPP] concentration will be 600 μM in a 1 ml final volume.) PNPP (substrate) stock solution: 100 mM PNPP in buffer 2. IN YOUR LAB NOTEBOOK, DRAW YOUR PREDICTION OF WHAT THEFOLLOWING GRAPHS MIGHT LOOK LIKEa. pH vs. V0b. TEMP vs. V03. OUTLINE THE BEST/MOST EFFICIENT WAY TO PREPARE

topic: Isoelectric Precipitation   Appearance of the mixtures containing casein mixed with different buffer solutions: (a) TestTube 1 containing Glycine-HCl buffer; (b) Test Tube 2 containing Acetate buffer; and (c) Test Tube 3containing Phosphate buffer. QUESTION: Based on the given results, what do you think is the isoelectric pH of casein? Briefly discuss your basis for determining it.

#2

Hello good day. In chemical examination of urine, please help me to answer of the test result both 1 and 2. Hoping for an answer. Thank you!

Help me answer the table thank you

Case Study Four   Case Study Assessing Dose Accuracy and Safety and Label Clarity   The Pharmacy Department of Miracle Hospital has ordered premixed solutions of cyclophosphamide 200 mg per 500 mL of NaCl 0.9%w/v injection from a specialty pharmacy compounding various parenteral products. The commercial 500 mL NS solution PVC bag has an overfill. The specialty pharmacy prepared the cyclophosphamide admixture by adding 10 mL of a reconstituted solution of 20 mg/mL cyclophosphamide to the 500 mL of NaCl 0.9%w/v PVC bag and labelled the IV admixture as 200 mg of cyclophosphamide per 500 mL. What is the concentration per millilitre of the cyclophosphamide admixture? (Napra 3.1)         What volume of this admixture would you draw-up in a syringe to deliver a 40 mg dose? (Napra 3.1)         What potential error could result if the premixed cytotoxic admixture is used? (Napra 6.1, 6.2, 9.2)         Case Study Having Knowledge of the Clinical Use of Specialty Parenteral Solutions   Mark…

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PART: G,H,I

Number 1

Case Study: Case Study: Catalase Activity Catalase HO2 (0 + O, (9) Catalase is an enzyme that converts hydrogen peroxide (H,O) to oxygen and water.

Will UPVOTE Answer the following: 1. What is the effect of boiling in the test tube with starch solution and saliva? 2. What is anaerobic oxidation? In your observation, which is the Hydrogen acceptor in the experiment, methylene blue or milk? 3. If the sample turned to blue or black upon the addition of I2 solution, is the honey genuine or not? Explain your answer 4. What changes do you think take place as the color changes upon the addition of phenol, pyrogallol or catechol and guac solution to the potato extract? 5. Differentiate an enzyme activator from an indicator

Lab: Isolation of beta-amylase from sweet potato Objective: To isolate the enzyme β -amylase using sweet potato as a source Materials Required:  Sweet potato.Knife/peeler.Mortar and Pestle.A Blender.Blue capped tubes.20mM sodium Phosphate buffer  at pH 7.Vortexer.  Procedure:  1.Take a clean sweet potato and peel the skin off.2.Weigh the peeled sweet potato and note the weight.3.The sweet potato is cut into small pieces and transferred into a mortar and pestle.4.The pieces are crushed and then transferred into a blender.5.Add 40 ml of cold 20mM sodium phosphate buffer saline. Blend it until  it forms a paste.6.Gently transfer the potato slurry into a blue capped tube.7.Allow the enzyme to extract over a 1 hour period at room temperature, with frequent vigorous stirring on a vortex mixer.8.Then the extract is filtered using a GF A glass fibre filter and the filtrate is collected in a new blue capped tube.9.Centrifuge the filtrate at 12000rpm for 20 minutes at 4 degree Celsius.10. After…

what departments in the laboratory will these tests go to?   not graded just an excercise

9 and 11 please.

Materials - 2021FA-CHM-1O X Bb 4427577 d-fleet02-xythos.content.blackboardcdn.com/6086c260d7e8f/4427577?X-Blackboard-Expiration=1633143600000&X-Blackboard-Sig 6 / 9 100% Exercise 1: Standard Curve for Protein Measurements: A standard curve for protein concentration is often created using known concentrations of bovine serum albumin (protein). This process is called the Bradford Assay; it is a colorimetric assay. A special reagent turns blue when it binds to amino acids present in protein. The intensity of the color is best measured with a spectrophotometer (a device for comparing two light radiations, wavelength by wavelength). In the case of the Bradford Assay the greater the absorbance, the higher the protein concentration. A series of tests were performed on some samples and spectrophotometer: following measurements were obtained using a Protein Concentration (mg/ml) Absorbance (A) 0.26 0.098 0.56 0.213 0.383 0.84 1.12 0.473 1.40 0.527 TASKS: 1. Enter the data into Excel - the…

9 and 10 please

How can I do these 3 questions?

Give detailed Solution with explanation needed please explain

Can I get help with these two questions?