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Pre-lab task 1: on the other side of this page, note the size range for typical Escherichia coli cells (length and width) and for most species of Anabaena (length and width). Be sure to include NLM/CSE-formatted references for the source of this information.

BONUS (15 points) The fallowing series of dilution was prepared from a specimen to determine the number of bacteria. There were 62 colonies on the agar plate prepared by transferring 0.3 ml from the tube number 4. Calculate the dilution factors for each tube. What is the cell concentration in the original specimen? Calculate the total number of cells in tube number 2. étv A) F12 F9 F10 F7 % & %3D delete 5 { T Y J K

LABORATORY REPORT: Aseptic Technique & Inoculation of Bacteria Guide Questions. 1. Why is direct flaming preferred when disinfecting loops and needles? 2. Why is it important to flame the entirety of the loop and not just the tip? What consequences can be seen when this process is not done correctly? 3. What is the difference between quadrant streak method A from method B? 4. Why do we use a pencil instead of a pen when labelling culture tubes or plates? Are there other alternatives in labelling? 5. Why is an inoculating needle used in Subculturing Techniques? Can a loop be used instead? NOTE: Please try to answer all of the question asked, i promised to give you a good ratings

Enumeration Questions: 1. You dilute an original sample 1:50. You then count the number of yeast cells in the 1:50 dilution. You count an average of 15 cells in the 1/25 mm2 sized boxes. What is the density, in cells/mL, of the original sample? 2. You count 91 cells in 7 of the 1/400 mm2 small boxes of the central grid on the hemocytometer. You are counting a 1:100 dilution of the original sample. What is the density of the original culture (in cells/mL) 3. You count 116 CFU on a pour plate. The plate was prepared by spreading 0.1 mL of a 1:10,000 dilution of the original sample. What is was the density of the original culture (in cells/mL)? 4. You have a culture of yeast that is at a density of 8x107 cells/mL. You dilute the sample 1:10, then 1:1,000 again, and finally you dilute the sample an additional 1:5. You add 0.1 mL of the final dilution to a spread plate. Assuming that most of the cells forming units do you expect to count on your pour plate the next day? the original culture…

S. epidermidis ~1 micron • Note: just focus on the round shape bacteria only (The pink color should be ignored for this lab)

Answer the following questions:   1. Define a bacterial colony.   2. What is the difference between macroscopic and microscopic appearance of bacteria?   3. State the three standard terms used to describe single colonies on agar plates.   4. State and define the three types of growth that may be seen in a broth culture.   5. State three basic shapes of bacteria.   6. State and describe the different arrangements of cocci.   7. What is the difference between true motility and Brownian motion?

First page is reference for number 4-7

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Fit to 3. A group of students wants to study the effects of temperature on bacterial growth. To get bacteria, they leave petri dishes of nutrient agar open on a shelf. They then put the dishes in different places: an incubator (37 degrees C) a lab room (21 degrees C), a refrigerator (10 degrees C) and a freezer (0 degrees C). Bacterial growth is measured by estimating the percentage of each dish covered by the bacteria at the end of a three day growth period. a) What is the independent variable? b) What is the dependent variable? c) List two (or more) ways to improve this experimental design.

Sample Station 1 This is an Eosin Methylene Blue (EMB) streaked with a pure culture. Looking at the growth and color in the image, answer the following: A. What is the expected Gram reaction and morphology of this organism? B. What does the color of the growth tell you about the organism's ability to ferment lactose to acids?

LAB QUESTIONS – ASSESSMENT AND CRITICAL THINKING 1. When is a simple negative stain used? 2. Why do microorganisms remain unstained in the simple negative staining procedure? 3. What information can be obtained from a simple positive staining procedure? 4. What is the difference between a simple and differential stain? 5. What is the reagent and purpose for each of the following gram stain steps? a. Primary stain b. Mordant c. Decolorizer d. Counterstain 6. Which step is the most crucial or most likely to cause poor results in the Gram stain? Why? 7. What part of the bacterial cell is most involved with Gram staining, and why? 8. What cell wall component is responsible for the acid-fast property of mycobacteria? 9. Is a gram stain an adequate substitute for an acid-fast stain? Why or why not? 10. Which area on a streak plate will contain the greatest amount of growth? The least amount of growth? Explain your answers. 11. What is a colony (as viewed on an agar plate)? How can a pure…

4-5 Please look at the top for more information. This is only thing was given

answer the following questions with book reference. 1. What is meant by a bacterial “colony” or colony-forming unit? 2. Why are colonies that develop on a heavily seeded plate smaller than those that appear on a sparely seeded plate? 3. What are some of the uses and advantages of the ff.:a. Agar plateb. Agar slantc. Broth culture 4. What are the advantages and limitations of studying bacteria by means of the Culture method? 5. Why is it desirable that most cultures be inspected after 15 to 18 hours of incubation? 6. Why should culture media after inoculation be incubated at an optimal temperature immediately? 7. Describe other types of aerobic jars and anaerobic methods.

Help I need to find what kind of Bacterium is this.

Sample Station 2 • This is micrograph of a Gram stained bacterium. Look at the cells indicated by arrows, answer the following: A. What is the cell morphology? B. What is the cell arrangement? C. What is the Gram reaction of this organism?

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Unanswered If you have a stock culture of Tetrahymena that has 100 cells/ml, how much stock culture would you use to make a 10 ml culture with a concentration of a 10 cells/ml? Type your numeric answer and submit Unanswered A Sul Fullscreen 14 JAN 24

Please answer questions 2-4 in the attached photo

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Questions 1. Explain why it was important to transfer the smallest colonies of yeast to the selective media plates.

Minute Timed PX B 4 5 6 rutgers.instructure.com/courses/207533/external_tools/retrieve?display=full_width&url=https%3A%2F%2Frutgers.quiz-Iti-iad-prod.instructure.com%2Flti... 7 8 9 10 11 12 13. 3 Quizzes 2 X Previous + 01:18:44 < Time Remaining Return 1 point The PCCE of the respiratory tract helps capture inhaled debris and pathogens which are then absorbed into the bloodstream and filtered out by the liver for elimination through the digestive system. True False U Nex d Next

Please answer fast What is this organism? And what other test could be done to confirm it's identification?   1.   Gram stain - positive cocci, chains   Hemolysis- gamma   BE - positive   NaCl - positive   Catalase - weakly positive   2.   Gram positive cocci, chains   NaCl - negative   BE - negative   Hippurate - positive   Hemoylsis - beta   Catalase - negative

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covert the procedure to passive voice and past tense

Question:- How to perform a bactericidal test bactericidal test of Dental Pulp Stem Cell (DPSC)?

Please you the first image as an example to fill the gaps on the 2nd image .please no explanation, just fill in the gap . Thanks

d-6f06-490d-b3d3-cb18767c100 Post-Lab: Streak Plate Isolation Homework. Due in 12 hours 2/5 answered Unanswered Post-Lab: PIV 2.5 Homework Unanswered How do colonies of E. coli differ from those of P. mirabilis? inancwarnd