BIO250 Lab 2 micro pdf

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“ Lab 2 Culturing & Aseptic Technique BIO250L ” Student Name: Brandon Estelle Access Code (located on the lid of your lab kit): AC-GIF4SU Lab Report Format Expecta0ons U"lize college level grammar and professional forma4ng when comple"ng this worksheet. Submissions without proper forma4ng, all required photos or sufficient responses will be rejected. Pre-lab Ques>ons 1. This lab includes two experiments that each look at different aspects of culturing microorganisms onto growth media. What concepts does each experiment focus on? Why do you think these concepts are important to the field of microbiology? THE CONCEPTS EXPERIMENTS FOCUS ON ARE VIRUSES, BACTERIA, ALGAE, ETC: 2. In this lab, the experiments will allow or call for the use of four inoculaVon tools. List these tools and provide a scenario in which you would use each tool. ○ Pipets ○ Agar plate ○ InoculaVng loop ○ Culture tube 3. Describe a piece of equipment used in biotech labs to extend the growth pa\ern of microbes. Focus parVcularly on those used with E. coli used to make human insulin. Incubators because they help bacteria cultures grow at ideal temperatures. 4. You’re a physician trying to isolate bacterial colonies from the human gut in an a\empt to diagnose a gastrointesVnal infecVon. You streak your sample on a growth media containing glucose, amino acids, and salts containing both sulfur and phosphorus with a pH of 7 . You incubate the plates in aerobic condi>ons at 37 ˚C for three days, at which point you can see clear bacterial colonies forming on the plate. Would you feel confident in staVng that you had successfully cultured all the bacteria from your gut sample? Why or why not? No personally I would not feel comfortable trading the paVent because I would only be seeing the aerobes on the culture. Also, because the anaerobic bacteria will not grow in this experiment. EXPERIMENT 1: AGAR PLATE PREPARATION AND BACTERIAL INOCULATION Introduc>on Ques>ons
“ Lab 2 Culturing & Aseptic Technique BIO250L ” 1. Describe the objecVves of Experiment 1. State the goal of the experiment and the learning outcome that it is trying to convey to you as a microbiology student. The main objecVve is to see how much bacteria growth can be on regular household use items. 2. Proper asepVc technique is crucial to ensuring the growth of pure bacterial colonies. What will you do in this lab to demonstrate that you are using proper asepVc technique? Proper hand hygiene, no food or drinks near lab, using the provided sterile equipment. In a laboratory sebng, what are three ways you can properly sterilize culturing equipment? ○ Dry heat ○ Wet heat ○ UV radiaVon or chemical solvents 3. What method of sterilizaVon will you use in this experiment? Dry heat 4. What results do you expect to see with this experiment? For example, do you expect to see growth from all surfaces or just some? Are there parVcular strains you expect from the surfaces you swabbed? I expect to see growth from some heavily used areas but not all as to some are kept clean. I expect to see more growth on the shoe bo\om than anything.
“ Lab 2 Culturing & Aseptic Technique BIO250L ” Data and Observa>ons In the table below, report your observed growth corresponding to each plate number and its corresponding source. Then provide a photo of your growth plates. Your data should have a quanVtaVve aspect to it, such as a count of the number of colonies that grew. Your data must also correspond to the photo you include for credit. Table 1: Colony Growth Provide a clear, high resoluVon photo of your plates ader incubaVon with the lids removed. For credit on this lab, your photo must show the growth that is reported in your tabulated data and must also include your handwri\en name in the background. Plate Number Source Growth (Color, Amount, Shape, etc.) 1 Teeth 0/ no growth 2 Fridge handle + Li\le growth round yellow, white colonies 3 Shoe bo\om ++ moderate growth about 7 colonies yellow/white 4 Control 0/ no growth 5 Airborne 0/ no growth
“ Lab 2 Culturing & Aseptic Technique BIO250L ” Sample 1 Sample 2 Sample 3 Control Airborne Contaminatio n
“ Lab 2 Culturing & Aseptic Technique BIO250L ”
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ce Final Part 2 (page 9 of 25) arkanacollege.edu/mod/quiz/attempt.php?attempt-502922&cmid%3353028page=8 BIology for Non-Sclence Majors I (BIOL-1308-TH-TRA, 2022-FA) Lecture Final Exam-Fail 20Z1 7 Lecture Final Part 2 - TEXARKANA COLLEGE My Courses Diogy ior NOn-Science iMiajuis i (DIOL-1308-TH-TRA, 2022 Question 9 Not yet answered Points out of 1.00 P Flag question Which sequence correctly lists the different levels of biological organization, from the smallest and simplest to the largest and most complex? Select one: O a. cells-organs-tissues-organ systems-organism O b. cells-tissues-organs-organ systems-organism Oc. tissues-organs-organ systems-organisms-cells O d. tissues-cells-organs-organ systems-organism ime left 0:40:08 DELL & @ #3 $4 8 2 3 4 P W e t y h k g
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