Assignment - DNA (14 pts

common-assessment-delivery/start/4797160920?action=onresume&submissionld%-468979669 English 9 Student D. S ddddddddddddddd... Type of Genetics Cr. S TEWWG Chapter DNA Protein Synthesis Test Chromosome Free Nucleotides DNA Polymerase Leading Strand Original- (Template DNA) Helicase Lagging Strand Replication Fork Adenine Thymine Guanine Cytosine DNA Polymerase Original (Template) DNA Strand 1. This diagram is showing the process of 2. 1f the original template strand reads: TTACGCAGT, DNA polymerase would add nucleotides in the order: 4 5 6 7 8. 9 10

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Activity 2. You COMPLETE Me! Objective: Identify the methods used for inserting plasmids into the host cells. What you need: pen and paper What to do: On a separate sheet of paper, copy and complete the key points of the methods used to introduce plasmids into the host cells. Choose your answers from the box gene gun biolistics electric shock mammalian cells plasmid insertion by Heat Shock Treatment increase the pore sizes of their plasma membranes (2) competent plasmid DNA Heat Shock electroporation There are three methods on introducing plasmids into the host cells namely: (1)_ (3) Biolistics uses a (4)_ to fire DNA-coated pellets on plant tissues. Plasmid insertion by Heat Shock Treatment is a process wherein target cells undergo a The pretreatment is said to make the cells (6). pretreatment procedure to (5) the introduction of the (7)_ plasmid at about 4°C for about 30 minutes and the plasmids concentrate near the cells during After the pretreatment, the cells are incubated with…

BIOTECHNOLGY Date: Name: Instructor: Section/Group:. POST-LAB QUESTIONS 1. In one or two sentences, summarize the technique of gel electrophoresis. 2. How does the process of gel electrophoresis separate DNA fragments? 3. Why is the fact that DNA has a negative charge so important in the gel electrophoresis process? Biotechnology 165

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Assignment_12.pdf - Adobe Reader File Edit View Window Help Оpen 1 / 1 99,1% Tools Fill & Sign Comment 3. Recombinant protein is produced by a genetically engineered strain of Escherichia coli during cell growth. The recombinant protein can be considered a product of cell culture even though it is not secreted from the cells; it is synthesized in addition to normal E. coli biomass. Ammonia is used as the nitrogen source for aerobic respiration of glucose. The recombinant protein has an overall formula of CH1.5500.31N..25. The yield of biomass (excluding recombinant protein) from glucose is measured as o.48 g/g; the yield of recombinant protein from glucose is about 20% of that for cells. How much ammonia is required? What is the oxygen demand? If the biomass yield remains at 0.48 g /g, how much different are the ammonia and oxygen requirements for wild-type E. coli that is unable to synthesize recombinant protein? а. b. с. 24°C 08:39 Berawan 27/04/2022

Activity 3: Short Essay Directions: Choose two questions to answer. You may use a separate sheet to complete the task. 1. Ascorbic acid (Vitamin C) is a soluble vitamin in the human body. Justify your answer. 2. Why can termites eat wood barks and other thick and hard parts of plants? Explain your answer. 3. Is using steroids good in the human body? Why or why not? 4. What is the difference between DNA and RNA?

Complete the sentences. Each term may be used more than once. In a circular bacterial chromosome, the structure of DNA is a If DNA is twisted in the Overwinding results in If DNA is twisted in the Underwinding results in One effect of double helix. direction, it becomes overwound. supercoiling. direction, it becomes underwound. supercoiling. double helix. Answer Bank positive negative right-handed left-handed supercoiling in bacterial chromosomes is to promote separation of the two strands of DNA in the

ACTIVITY 3: Analyze Me! Direction: Draw a smiling face (O) if the statement is an importance of genetic engineering and (O if not. 1. Genetic engineering has been used to mass-produce insulin. 2. Genes and other genetic information from a wide range of organisms are transformed into bacteria for storage and modification, creating genetically modified bacteria in the process. 3. Some genetically engineered animals become an important source of new drugs. 4. Many genetically engineered crops are being used by many farmers now. 5. Genetic engineering is used to produce more needed genetically engineered medicines.

Write short answers. i. RNA not exists in double stranded from as DNA. Why?

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B. Answer the following questions briefly. 1. How do we know that complementary base pairs in DNA really exist? 2. Compare and contrast replication, transcription and translation based on template used, direction of synthesis, protein/enzyme requirements during initiation, elongation and termination and products. CRITERIA of Replication Transcription Translation comparison 1. Template used 2. direction of synthesis 3. protein enzyme requirements a. Initiation b. Elongation c. Termination 4. Products 3. How do we know that expression of the information encoded in DNA involves an RNA intermediate?

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Questions:- 6. In prokaryotes, which DNA polymerase is primarily responsible for filling in the gaps inDNA that are generated during nucleotide excision repair?7. Why can UV induced pyrimidine dimers undergo direct reversal in the light but not inthe dark?

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Please help and explain the following question...thank you!   Describe instances when a DNA mutation would not alter the function of a protein. Describe instances when a DNA mutation would alter the function of a protein

Legrning Task 2: Make a.model of a DNA template to deternmine the seguence of bases in th new DNA strahds Then, answer the gulde questions that follow. Materials: crayons. Scissors, paste/tape, used folder or illustration board Procedure: Use the pattern of the DNA templater (attached to this LP). Color code, phosphate = blue, deoxyribose sugar = green, nitrogenous base as follows: adenine= yellow, thymine = pink, guanine = violet, cytosine = red. And cut the shapes of each nucleotides. Buld a model of a strand of a DNA molecule. The strand should contain 6 base" rungs" following the given order of the nucleotides: Guanine, Adenine, Cytosine, Thymine, Cytosine, Guanine. Tape the cutout pattern to form the nucieotides. This will represent the left half of DNA. Make a complementary strand that you made in step 3. Tape the cut -out pattern again forming the nucieotides for the second strand of the DNA molecules. Match the bases of the first strand and the second strand. Do not tape across…