LAB REPORT #2 Fundamental Skills Madrid

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Jan 9, 2024

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Arlene Madrid January 31,2023 BIOL 3226_21 Professor. Benzecry Lab Report #2 1. Define the following terms: -Batch culture system-A batch culture system is a way to grow microorganisms in a closed system where all of the necessary nutrients and conditions are added at the start and stayed the same throughout the growth period. Large-scale microorganism production and laboratory research both frequently use this kind of culture system. -Colony formation units (FCU)-The quantity of living bacteria or other microorganisms in a sample is measured in terms of colony forming units, or CFUs. Spreading a sample of the culture onto a solid growth media and counting the number of colonies that appear will yield this number. CFUs are a measurement of bacterial growth and a sign of how well antimicrobial treatments are working. -Contaminant-A microbe that is not present in the patient but can grow in culture and produce false results. -Culture media-In the laboratory, culture media is a material that helps microorganisms grow. A variety of nutrients, including carbohydrates, amino acids, vitamins, and minerals, can be found in culture media, which can be either solid or liquid. These nutrients assist the growth of particular microbes. Microorganisms like bacteria, yeast, or fungi are grown using various types of culture media. -Disinfectant-A disinfectant is a substance used to eradicate microorganisms from surfaces or objects, including bacteria, viruses, and fungi. In order to preserve hygiene and stop contamination, disinfectants are frequently employed in hospital settings, as well as in facilities that process food and water. -Incineration-In order to limit the amount of waste and get rid of germs, organic materials like trash or medical waste are burned at high temperatures as part of the incineration process. At order to stop the spread of disease, infectious trash like soiled bandages or sharps is frequently burned in healthcare facilities. Burning is another method used in microbiology to sterilize supplies and tools for the lab. -Inoculum- The process of transferring microbes from one medium to another is known as inoculation and the sample being transferred.

-Microbial mat- A microbial mat is a stratified, thick population of microorganisms that can be found in hot springs, soil, freshwater, or any number of other habitats. They are produced as a result of interactions and interdependencies between various species, such as bacteria, fungus, algae, and archaea. -Open culture system-An open culture system used in microbiology and biotechnology is referred to as a "open culture system" because the culture is exposed to the atmosphere, allowing for the interchange of gases and other materials between the culture and the environment. -Optical property- Optical properties refer to a material's physical properties that influence how it responds to light. In disciplines like optics, photonics, and materials science, certain characteristics, such as refractive index, absorption, and transmission, are significant. -Pure Culture-A pure culture is a sort of laboratory culture for microorganisms that contains just one kind, as opposed to mixed cultures, which include different kinds. Purified cultures are frequently obtained using methods like streaking or serial dilution. -Stock culture- A stock culture is a culture of microorganisms that is kept alive and saved for use as an inoculum in the future. Stock cultures, which are used to inoculate new cultures or studies, are normally preserved in liquid or freeze-dried form. -Sub-culture- A sub-culture is the process of transferring cells or inoculum from an existing culture to new growing medium to create a new culture. In microbiology, sub-culturing is frequently employed to retain or sustain cultures or to isolate pure strains of microorganisms. -Transient flora-The presence of microorganisms in and on our body have been recognized either as being temporary in nature. -Turbidity-The distribution of microorganisms throughout the liquid sample. 2.Define and describe the proper usage of the following culture media: -Agar deeps-Agar deeps are spherical tubes with screw-on lids that are filled with nutritional agar media that has solidified. They are used to cultivate and preserve numerous bacterial cultures in order to keep them alive for extended periods of time. Agar deeps are particularly helpful for preserving uncommon or slowly-growing microorganisms as well as subcultures that will be employed in future research. -Agar plates-Agar plates are flat, circular dishes used to cultivate and confine bacterial colonies. They are filled with solidified nutritional agar media. They are employed in a number of microbiological processes, including bacterial streaking, colony counting, and testing for

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