BIO201 Lab 4

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Jan 9, 2024

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Lab 4 Diffusion and Osmosis BIO201L Student Name: Ani Harutyunyan Access Code (located on the lid of your lab kit): AC-BX89CU Lab Report Format Expecta0ons U"lize college level grammar and professional forma4ng when comple"ng this worksheet. Submissions without proper forma4ng, all required photos or sufficient responses will be rejected. Pre-lab Ques>ons 1. Define the following terms. a. hypertonic soluAon: a soluAon containing more solvent than solute b. hypotonic soluAon: a soluAon that has a solute concentraAon lower than that of the cell c. isotonic soluAon: Isotonic soluAons are characterized by having the same concentraAon of solutes and water as the cytoplasm within cells. When cells are immersed in an isotonic soluAon, they maintain their shape as there is no net water movement— no change to cell size 2. Define osmoAc pressure. OsmoAc pressure refers to the necessary hydrostaAc force that must be exerted on a soluAon to halt the inward movement of water through a semi-permeable membrane. 3. Explain what would happen to a cell placed in a hypertonic soluAon. Water traverses out of the cell through the plasma membrane, leading to the cell's shrinkage.
Lab 4 Diffusion and Osmosis BIO201L EXPERIMENT 1: DIFFUSION THROUGH A LIQUID Introduc>on Ques>ons 1. State the molecular weight of the blue dye in grams per mole. 792.9g or 793g(rounded) per mole 2. State the molecular weight of the red dye in grams per mole. 495.9g to 496(rounded) per mole 3. Why does this experiment require two dyes of different molecular weights? Answer this quesAon, ensuring you explain the purpose of these two dyes in relaAon to the experiment. In the experiment, two dyes of disAnct molecular weights are employed to assess the permeability of the potato cell membrane to differently sized molecules, thereby evaluaAng its selecAvity and permeability characterisAcs.
Lab 4 Diffusion and Osmosis BIO201L Data and Observa>ons Record the diameters you observed for both dyes in the table below. Table 1: Rate of Diffusion in Corn Syrup Calculate the speed of diffusion and present this, along with the required data, for both dyes below in Table 2. To calculate the hourly diffusion rate, mulAply the total distance diffused by 30. Table 2: Speed of Diffusion of Different Molecular Weight Dyes Time (sec) Blue Dye Red Dye 10 1cm 1.2cm 20 1.5cm 1.5cm 30 1.8cm 2cm 40 1.9cm 2.5cm 50 2cm 2.7cm 60 2cm 2.8cm 70 2.1cm 2.9cm 80 2.5cm 3.1cm 90 2.7cm 3.3cm 100 2.8cm 3.3cm 110 2.9cm 3.4cm 120 3cm 3.6cm
Lab 4 Diffusion and Osmosis BIO201L Results and Discussion 1. Examine the plot below. How well does it match the data you took in Table 1? The chart illustrates that over Ame, the red dye consistently diffused further than the blue dye, suggesAng a more efficient diffusion process, which corroborates and deepens our iniAal understanding. 2. Which dye diffused the fastest? red dye Structure Molecular Weight Total Distance Traveled (mm) Speed of Diffusion (mm/hr)* Blue Dye 793g/mole 31mm 930mm/hr Red Dye 496g/mole 34mm 1020mm/hr
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