BIO201 Lab 4
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Lab 4 Diffusion and Osmosis
BIO201L
Student Name:
Ani Harutyunyan
Access Code (located on the lid of your lab kit):
AC-BX89CU
Lab Report Format Expecta0ons
U"lize college level grammar and professional forma4ng when comple"ng this worksheet.
Submissions without proper forma4ng, all required photos or sufficient responses will be rejected.
Pre-lab Ques>ons
1.
Define the following terms.
a.
hypertonic soluAon:
a soluAon containing more solvent than solute
b.
hypotonic soluAon:
a soluAon that has a solute concentraAon lower than that of the cell
c.
isotonic soluAon:
Isotonic soluAons are characterized by having the same concentraAon of solutes and
water as the cytoplasm within cells. When cells are immersed in an isotonic soluAon,
they maintain their shape as there is
no net water movement— no change to cell size
2.
Define osmoAc pressure.
OsmoAc pressure refers to the necessary hydrostaAc force that must be exerted on a soluAon to
halt the inward movement of water through a semi-permeable membrane.
3.
Explain what would happen to a cell placed in a hypertonic soluAon.
Water traverses out of the cell through the plasma membrane, leading to the cell's shrinkage.
Lab 4 Diffusion and Osmosis
BIO201L
EXPERIMENT 1: DIFFUSION THROUGH A LIQUID
Introduc>on Ques>ons
1.
State the molecular weight of the blue dye in grams per mole.
792.9g or 793g(rounded) per mole
2.
State the molecular weight of the red dye in grams per mole.
495.9g to 496(rounded) per mole
3.
Why does this experiment require two dyes of different molecular weights? Answer this
quesAon, ensuring you explain the purpose of these two dyes in relaAon to the experiment.
In the experiment, two dyes of disAnct molecular weights are employed to assess the
permeability of the potato cell membrane to differently sized molecules, thereby evaluaAng its
selecAvity and permeability characterisAcs.
Lab 4 Diffusion and Osmosis
BIO201L
Data and Observa>ons
Record the diameters you observed for both dyes in the table below.
Table 1: Rate of Diffusion in Corn Syrup
Calculate the speed of diffusion and present this, along with the required data, for both dyes below in
Table 2. To calculate the hourly diffusion rate, mulAply the total distance diffused by 30.
Table 2: Speed of Diffusion of Different Molecular Weight Dyes
Time (sec)
Blue Dye
Red Dye
10
1cm
1.2cm
20
1.5cm
1.5cm
30
1.8cm
2cm
40
1.9cm
2.5cm
50
2cm
2.7cm
60
2cm
2.8cm
70
2.1cm
2.9cm
80
2.5cm
3.1cm
90
2.7cm
3.3cm
100
2.8cm
3.3cm
110
2.9cm
3.4cm
120
3cm
3.6cm
Lab 4 Diffusion and Osmosis
BIO201L
Results and Discussion
1.
Examine the plot below. How well does it match the data you took in Table 1?
The chart illustrates that over Ame, the red dye consistently diffused further than the blue dye,
suggesAng a more efficient diffusion process, which corroborates and deepens our iniAal
understanding.
2.
Which dye diffused the fastest?
red dye
Structure
Molecular Weight
Total Distance
Traveled (mm)
Speed of Diffusion
(mm/hr)*
Blue Dye
793g/mole
31mm
930mm/hr
Red Dye
496g/mole
34mm
1020mm/hr
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