BIO250 Lab 7
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Lab 7 Microbial Genetics & Genetic Engineering
BIO250L
”
Student Name:
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.
Access Code (located on the lid of your lab kit):
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.
Lab Report Format Expectations
Utilize college level grammar and professional formatting when completing this worksheet
.
Submissions without proper formatting, all required photos or sufficient responses will be rejected
.
Pre-lab Questions
1.
Why are the concepts in Lab 7 important in the field of microbiology?
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2.
Which DNA nitrogenous bases pair with each other?
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3.
Which bases are purines, and which are pyrimidines?
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4.
How is DNA information used to make proteins? What are the steps of this process, and what
are the significant pieces of cell machinery that participate in this process?
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5.
What occurs during each of the three steps involved in the PCR cycle? How has the use of PCR
changed biotechnology?
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6.
What are the three-nucleotide sequences that correspond to a specific amino acid called?
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7.
How could you take a known sequence of amino acids and use it to create an artificial gene?
Hint: Think about this question in the context of Question 6, above.
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Lab 7 Microbial Genetics & Genetic Engineering
BIO250L
”
EXPERIMENT 1: EXTRACTION OF DNA
Introductory Questions
1.
What is the purpose of the following reagents in the experiment?
a.
Salt (in the DNA extraction solution):
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b.
Detergent (in the DNA extraction solution):
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c.
Ethanol:
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2.
What do you expect to see when you perform Step 7 in this experiment?
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Data and Observations
Insert a photo of your DNA collected on the wooden stir stick. Include your name and access code
handwritten in the background of your photo. The photo must show the DNA as denoted in the lab,
using the wooden dowel to remove it from the vial
.
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