BIO250 Lab 7

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Straighterline *

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MICROBIOLO

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Biology

Date

Jan 9, 2024

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docx

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4

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Lab 7 Microbial Genetics & Genetic Engineering BIO250L Student Name: Click here to enter text . Access Code (located on the lid of your lab kit): Click here to enter text . Lab Report Format Expectations Utilize college level grammar and professional formatting when completing this worksheet . Submissions without proper formatting, all required photos or sufficient responses will be rejected . Pre-lab Questions 1. Why are the concepts in Lab 7 important in the field of microbiology? Click here to enter text. 2. Which DNA nitrogenous bases pair with each other? Click here to enter text. 3. Which bases are purines, and which are pyrimidines? Click here to enter text. 4. How is DNA information used to make proteins? What are the steps of this process, and what are the significant pieces of cell machinery that participate in this process? Click here to enter text. 5. What occurs during each of the three steps involved in the PCR cycle? How has the use of PCR changed biotechnology? Click here to enter text. 6. What are the three-nucleotide sequences that correspond to a specific amino acid called? Click here to enter text. 7. How could you take a known sequence of amino acids and use it to create an artificial gene? Hint: Think about this question in the context of Question 6, above. Click here to enter text.
Lab 7 Microbial Genetics & Genetic Engineering BIO250L EXPERIMENT 1: EXTRACTION OF DNA Introductory Questions 1. What is the purpose of the following reagents in the experiment? a. Salt (in the DNA extraction solution): Click here to enter text. b. Detergent (in the DNA extraction solution): Click here to enter text. c. Ethanol: Click here to enter text. 2. What do you expect to see when you perform Step 7 in this experiment? Click here to enter text. Data and Observations Insert a photo of your DNA collected on the wooden stir stick. Include your name and access code handwritten in the background of your photo. The photo must show the DNA as denoted in the lab, using the wooden dowel to remove it from the vial .
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