Amino Acid Lab Write-up 1

Lab 3 Classes of biological molecules (e.g. proteins) _______________________________________ Monomers ______________________________________________________________________________ Polymers ______________________________________________________________________________ Dehydration Synthesis _____________________________________________________________________ Hydrolysis ______________________________________________________________________________ Enzyme ______________________________________________________________________________ Catalyst _____________________________________________________________________________ Substrate _____________________________________________________________________________ Product ______________________________________________________________________________ Indicator _____________________________________________________________________________ Starch ______________________________________________________________________________ Amylase…

Report Sheet: Proteins (page 3) What kind of protein is casein? Name: POST-LABORATORY QUESTIONS: What is the isoelectric point of casein? What is acid in our sour milk? How is it produced? What type of chemical grouping is present in all proteins? How many of these chemical groupings must be present in a molecule to give a positive Biuret test? Give the principle involved and the chemical structure responsible for the positive Biuret Test? Can you distinguish proteins and other amino compounds by the Ninhydrin test? Explain. 30 | Biochemistry

Name plz

Label B only

Hd 12 10 8 CO 6 st 4 2 0 0 0.5 not text. only, no text. only, no text. 1 1.5 NaOH Equivalents 2 2.5 You create this titration curve of an amino acid. How many buffering zones are there? Enter a number, no letters. 3 A What is the pka1 of this amino acid? Enter only digits What is the pka2 of this amino acid? Digits A A/ What is the pl of this amino acid? Digits A/

Explain the advantage of using Fluorescence Recovery After Photobleaching (FRAP) in determining protein localisation I notes = Notes Comments MAY 7.

Structures: Purine Pyrimidine

Identification. Write in CAPITAL LETTERS. Wrong spelling, wrong.  Proteins are classified according to function,examples of these classes are: _____________________, ________________________, ____________________________, ______________________________, ____________________________, ___________________________, _____________________________.

1. Draw chemical structure of 20 amino acids found in the proteins (Do Not cut-and-paste images from the internet) 2. Write the full name, 3-letter, and 1-letter codes for 20 amino acids 3. Classify 20 amino acids according to the chemical characteristics Please answer very soon will give rating surely Complete Answer needed

Choline test procedure (chemical reagents added), positive results/observations, and positive for (provide details like functional group responsible and list of lipids that will yield positive results)

Label the following components (Number 1-5) Thank you so much

Name: Report Sheet: Proteins (page 4) Would the Ninhydrin test be reliable in determining the presence of proteins? Explain. Will any amino acid having an aromatic ring give positive Xanthoproteic test result under identical conditions? Why? Do most proteins give a positive Xanthoproteic test? Why or why not? For what chemical group is Millon's reagent a test? Write the formula and the name of the amino acid for which this reaction is a test. What chemical grouping is responsible for a positive Hopkin's-Cole Test? What element in the amino acid is detected to be present in the lead acetate test? 31 | Biochemistry

Lab Test Worksheet Fill in the description (what is the lab for), normal values and clinical significance there may be if values are above/below normal https://labtestsonline.org Lab Test Lipid Panel Description Normal Values Clinical Significance if Above/Below Normal Values Total cholesterol High-density lipoprotein cholesterol (HDL-C) Low-density lipoprotein cholesterol (LDL-C) Triglycerides Lab Test Description Normal Values Clinical Significance if Above/Below Normal СВС Complete Blood Count Red blood cells Values White blood cells Hemoglobin Нematocrit Platelets

COLOR REACTION OF PROTEINS Question Guide: 1. Write the positive result, the chemical group responsible for the positive and the importance of each test. Test Positive Result Chemical Group Biuret Ninhydrin Xanthoprotei Millon's Hopkins-Cole Lead Acetate Pauly Bromine Water Sakaguchi

#2

Activity 6.3.1: Complete the Table  Polysaccharide Unique Feature Important Function Chondroitin     Heparin     Hyaluronate     Dermatan Sulfate     Keratan Sulfate     Mucin

Group of lipids identified in Acrolein Test

Name the following lipid. thank u

9L.9

A mixture of lipids containing phosphatidic acid, cholesterol, testosterone, phosphatidylserine, andphosphatidylethanolamine was applied to a hydrophobic interaction chromatography column. Thecolumn was washed with a high salt buffer, and the lipids were then eluted with decreasing saltconcentrations. In what order would the lipids be eluted from the column? Explain your answer.

Give detailed Solution with explanation (don't give Handwritten answer)identify all

answer 1

name of steroid name of solvent color reaction principle involved/ reaction mechanism Androsterone sulfuric acid     progesterone sulfuric acid     methyl testosterone sulfuric acid     prednisone sulfuric acid     Above are the list of steroidal compounds and the solvent used to test their color reactions. Determine the color reactions and the principle involved or color reaction mechanism of each steroid.

Table 4- Determination of the optimum pH of catechol oxidase enzyme Potato extract (mL) Tube # 2d 3d 4d 5d 6d 7d dH₂O (mL) 0 0 0 0 0 0 Catechol (mL) 6 6 6 6 6 6 pH buffer added 4mL pH 2 4mL pH 4 4mL pH 6 4mL pH 7 4mL pH 8 4mL pH 10 1 1 1 1 1 1 Absorbance (0 mins) A: 0.010 Start Time: 3:24 A: 0.008 Start Time: 3:34 A: 0.023 Start Time: 3:36 A: -0.006 Start Time: 3:36 0.018 A: Start Time: 3:36 A: 0.011 Start Time: 3:36 Absorbance (after 10 mins in 40°C water bath) -0.030 A: Take reading at: 3:44 A: 0.154 Take reading at: 3:46 A: 0-105 Take reading at: 3:46 A: 0-132 Take reading at: 3:46 A: 0.074 Take reading at: 3:46 A: 0.018 Take reading at: 3!46 Q15) What is the enzyme's optimum pH(s)? Answer: Q16) Use the difference between 2nd and 1st absorbance to support your conclusion for the optimum pH. Subtract Omin from 10min absorbance -0.04 0.0012 0.082 0.138 0.056 0.007

Titration curve of an unknown amino acid The graph below shows a curve, which was obtained following titration of an unknown amino acid. Include a suitable descriptive title stating the identity of the unknown amino acid Use rectangles to precisely outline the regions in the graph where ionisable groups show buffering activity (base/acid ratio 1:10 to base/acid ratio 10:1); clearly associate the name of the ionisable group with the buffering regions; indicate within the graph all observable titration mid-points and all observable titration end-points and indicate estimates of the pKa of the three functional groups (do not provide pka values from the literature, you need to read the pka from the titration curve provided). Note that it is not possible to estimate the pka with more than one decimal precision due to limited resolution of the shown graph.

As2Cl3 chemical name

Insert Chart Table alpha carbon #1 R1 Text 8. Provided is a structural diagram of a segment of a protein. Which atoms are involved in the hydrogen bonding utilized in secondary structure? | Co Media Shape Peptide Bond Comment N Ca R2 alpha carbon #2